Summary
UV survival of E. coli strains with different sensitivities has been measured under several post-irradiation conditions, which have been found to influence only the efficiency of two known reactivation mechanisms.
Strains BB, B and Bs-1 (equivalent to fil+ and probably lon−) cannot use a reactivation mechanism which is active in K 12 (K-reactivation). Only a regulatory component is lacking, which can be substituted for by pantoyl lactone or heat treatment. B or BB cells in the growing state perform incomplete dark-reactivation; liquid holding or growth to the stationary phase restore it. K-reactivation also allows completion of darkreactivation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adler, H. I., andM. Engel: Factors influencing the survival of bacteria after exposure to ionizing radiation. J. cell. comp. Physiol.58, Suppl., 95–105 (1961).
—, andA. A. Hardigree: Analysis of a gene controlling cell division and sensitivity to radiation inE. coli. J. Bact.87, 720–726 (1964).
Alper, T., andN. E. Gillies: The relationship between growth and survival after irradiation ofEscherichia coli strain B and two resistant mutants. J. gen. Microbiol.22, 113–128 (1960).
Arber, W., u.C. Lataste-Dorolle: Erweiterung des Wirtsbereiches des Bakteriophagen λ aufEscherichia coli B. Path. et Microbiol. (Basel)24, 1012–1018 (1961).
Boyce, R. P., andP. Howard-Flanders: Release of ultraviolet light-induced thymine dimers from DNA inE. coli K12. Proc. nat. Acad. Sci. (Wash.)51, 293–300 (1964).
Clark, A. J., andA. D. Margulies: Isolation and characterization of recombination-deficient mutants ofEscherichia coli K12. Proc. nat. Acad. Sci. (Wash.)53, 451–459 (1965).
Demerec, M., andR. Latarjet: Mutations in bacteria induced by radiations. Cold Spr. Harb. Symp. quant. Biol.11, 38–50 (1946).
Garen, A., andN. D. Zinder: Radiological evidence for partial genetic homology between bacteriophage and host bacteria. Virology1, 347–376 (1955).
Harm, W.: On the relationship between host-cell reactivation and UV-reactivation in UV-inactivated phages. Z. Vererbungsl.94, 67–79 (1963).
—, andW. Stein: Vergleich der UV-Inaktivierung und Wärmeaktivierung von verschieden UV-empfindlichen Coli-Kulturen. Naturwissenschaften39, 212–213 (1952).
Hill, R. F., andE. Simson: A study of radiosensitive and radioresistent mutants ofEscherichia coli strain B. J. gen. Microbiol.24, 1–14 (1964).
Howard-Flanders, P., E. Simson, andL. Theriot: The exicision of thymine dimers from DNA, filament formation and sensitivity to ultraviolet light inEscherichia coli K 12. Mut. Res.1, 219–226 (1964a).
———: A locus that controls filament formation and sensitivity to radiation inEscherichia coli K 12. Genetics49, 237–246 (1964b).
Jacob, F., andE. Wollman: Sexuality and the genetics of bacteria. New York and London: Academic Press 1961.
Jagger, J., W. C. Wise, andR. S. Stafford: Delay in growth and division induced by near UV radiation inE. coli B and its role in photoprotection and liquid holding recovery. Photochem. Photobiol.3, 11–24 (1964).
Kaudewitz, F.: Inaktivierende und mutagene Wirkung salpetriger Säure auf Zellen vonEscherichia coli. Z. Naturforsch.14b, 528–537 (1959).
Kneser, H.: Repair of UV lesions and induction of λ-prophage, Virology (in press) (1966).
—,K. Metzger, andW. Sauerbier: Evidence of different mechanisms for UV reactivaton and “ordinary host cell reactivation” of phage λ. Virology27, 213–221 (1965).
Pettijohn, D., andP. Hanawalt: Evidence for replication of ultraviolet damaged DNA in bacteria. J. molec. Biol.9, 395–410 (1964).
Reich, E., A. J. Shatkin, andE. L. Tatum: Bacteriocidal action of mitomycin C. Biochim. biophys. Acta (Amst.)53, 132–149 (1961).
Roberts, R. B., andE. Aldous: Recovery from ultraviolet irradiation inEscherichia coli. J. Bact.57, 363–375 (1949).
Rörsch, A., A. Edelman, C. van der Kamp, andJ. A. Cohen: Phenotypic and genotypic characterization of radiation sensitivity inEscherichia coli B. Biochim. biophys. Acta (Amst.)61, 278–289 (1962).
Sauerbier, W.: The bacterial mechanism reactivating UV irradiated phage in the dark. Z. Vererbungsl.93, 220–228 (1962a).
—: Evidence for a nonrecombinational mechanism of host cell reactivation of phage. Virology16, 398–404 (1962b).
Setlow, R. B., andW. L. Carrier: The disappearance of thymine dimers from DNA: An error correcting mechanism. Proc. nat. Acad. Sci. (Wash.)51, 226–231 (1964).
Stein, W., andI. Meutzner: Reaktivierung von UV-inaktivierten Bacterium coli durch Wärme. Naturwissenschaften37, 167–168 (1950).
Van de Putte, P., C. Westenbroek, andA. Rörsch: The relationship between genecontrolled radiation resistance and filament formation inEscherichia coli. Biochim. biophys. Acta (Amst.)76, 247–256 (1963).
Witkin, E. M.: In: Repair from genetic radiation damage (ed.F. H. Sobels). Oxford, London, New York and Paris: Pergamon Press 1963.
—: Photoreversal and “dark repair” of mutations to prototrophy induced by UV light in photoreactivable and non-photoreactivable strains ofEscherichia coli. Mut. Res.1, 22–36 (1964).
Wulff, D. L.: The role of thymine dimer in the photo-inactivation of the bacteriophage T4v1. J. molec. Biol.7, 431–441 (1963).
Zampieri, A., andJ. Greenberg: Effect of heat and plating medium on survival ofEscherichia coli after treatment with radiomimetic chemicals. J. Bact.89, 931–936 (1965).
Zelle, M. R., andA. Hollaender: In: Radiation biology (ed.A. Hollaender), vol. II, p. 365. New York, Toronto and London: McGraw-Hill Book Co. 1954.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kneser, H. Dark- and K-reactivation in UV irradiatedEscherichia coli . Zeitschrift für Vererbungslehre 97, 102–110 (1965). https://doi.org/10.1007/BF00897489
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00897489