Summary
-
1.
Diploid six rowed barley seeds were soaked in water for 4, 8, 12, 16, 20, 24 and 28 hours, before treatment with 0.3% and 0.6% aqueous solution of ethyl methanesulfonate for 4 hours. Morphological, cytological and genetical changes induced were studied.
-
2.
For all criteria studied, 16 hours or 20 hours presoaking in water was found to increase the sensitivity of the seeds to the maximum. Highest mutation rate was obtained at this time of presoaking, when appropriate concentration was used.
-
3.
The proportion of chlorophyll to viable mutations was found to vary with various times of presoaking. At longer presoaking times, relatively higher proportion of viable mutations were obtained. This, however, was not associated with an increase in viable mutation rate.
-
4.
The chlorophyll mutation spectrum was also found to vary for a given dose of EMS following different presoaking times.
-
5.
The possible factors for the increased sensitivity at 16 to 20 hours soaking are discussed in light of similar studies with radiations as well as available microautoradiographic data pertaining to the sequence of chromosome duplication in germinating barley seeds. It is concluded that a metabolic factor like DNA duplication in the cell initials may be involved in bestowing this increased sensitivity during this presoaking period.
Similar content being viewed by others
Literature
Ehrenberg, L., Å. Gustafsson, andD. von Wettstein: Studies on mutation process in plants—regularities and intentional control. Proc. conference on Chromosomes, Wageningen, 1956, p. 131–159.
Freese, E. B., andE. Freese: On the mutagenic effect of alkylating agents. Proc. nat. Acad. Sci. (Wash.)46, 1584–1594 (1961).
Freese, E.: Molecular mechanism of mutations. In: Molecular genetics, vol. 1, p. 207–270 (Edit.J. H. Taylor). New York: Academic Press 1963.
Gaul, H.: Critical analysis of methods for determining the mutation frequency after seed treatment with mutagens. Genet. agrar.12, 297–318 (1960).
Kamra, O. P., S. K. Kamra, R. A. Nilan, andC. F. Konzak: The radiation response of soaked barley seeds. II. Hereditas (Lund)46, 261–273 (1960).
Latteral, R. L.: The influence of oxygen on the radiosensitivity of maize chromosomes during seed germination. Radiat. Res.14, 480 (1961).
Natarajan, A. T., G. Ahnström, andR. A. Pai: Studies of oxygen effect on radiation response of barley seeds. Proc. Second Intern. Congr. of Radiation Research, p. 256, 1962.
Natarajan, A. T., andL. Ehrenberg: (In preparation) (1964).
—, andM. D. Upadhya: Localized chromosome breakage induced by ethyl methanesulfonate and hydroxylamine inVicia faba. Chromosoma (Berl.)15, 156–169 (1964).
Ramanna, M. S., andA. T. Natarajan: The modification of hydrogen ion concentration and temperature on the chromosome breaking and mutagenic effects of monofunctional and bifunctional alkylating agents in barley (under publication).
Röbbelen, G.: Über die Unterschiede in den genetischen Folgen einer Röntgenbestrahlung verschiedenartiger Pflanzenzellen. Untersuchungen anArabidopsis thaliana (L.)Heynh. Z. Vererbungsl.93, 127–153 (1962).
Stadler, L. J.: Mutations in barley induced by X-rays and radium. Science68, 186–187 (1928).
Swaminathan, M. S., V. L. Chopra, andS. Bhaskaran: Chromosome aberrations and the frequency and spectrum of mutations induced by ethyl methanesulfonate in barley and wheat. Ind. J. Genet.22, 192–207 (1962).
Author information
Authors and Affiliations
Additional information
With 4 Figures in the Text
Rights and permissions
About this article
Cite this article
Natarajan, A.T., Shivasankar, G. Studies on modification of mutation response of barley seeds to ethyl methanesulfonate. Zeitschrift für Vererbungslehre 96, 13–21 (1965). https://doi.org/10.1007/BF00897231
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00897231