Summary
Host-cell reactivation (HCR) and UV-reactivation (UVR) were studied in phage T1, T3 and λ, using as host bacteriaE. coli B, C, andK12S, as well as their non-hostreactivating mutantsB s−1 (Ellison et al. 1960),C syn −(Rörsch et al. 1962), andK12S hcr −. The experiments gave further support to the idea that HCR is an enzymatic process. It repairs about 80 to 90 percent of otherwise lethal UV-lesions not only in phage DNA, but also in bacterial DNA. Thehcr − mutant isolated fromK12S for the purpose of this investigation, and thesyn − mutant of ColiC show a very small extent of HCR; they are not completely deficient for the HCR-enzyme.
A correlation exists between the occurrence of HCR and UVR. UVR is absent in those cases where no HCR is observed. In systems with residual HCR-activity (hcr − andsyn − cells) UVR is less pronounced and has its maximum at lower UV-doses than in systems with full HCR-activity. UVR occurs also in unirradiated host-reactivating cells, if a large number of additional UV-lesions is introduced by means of superinfecting homologous phage. This effect is not observed in non-hostreactivating strains. The hypothesis is discussed that UVR is not a specific repair phenomenon by itself, but is the result of inhibition of cellular processes tending to decrease the survival.
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Harm, W. On the relationship between host-cell reactivation and UV-reactivation in UV-inactivated phages. Zeitschrift für Vererbungslehre 94, 67–79 (1963). https://doi.org/10.1007/BF00895157
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DOI: https://doi.org/10.1007/BF00895157