Zeitschrift für Vererbungslehre

, Volume 94, Issue 1, pp 67–79 | Cite as

On the relationship between host-cell reactivation and UV-reactivation in UV-inactivated phages

  • Walter Harm


Host-cell reactivation (HCR) and UV-reactivation (UVR) were studied in phage T1, T3 and λ, using as host bacteriaE. coli B, C, andK12S, as well as their non-hostreactivating mutantsBs−1 (Ellisonet al. 1960),C syn(Rörschet al. 1962), andK12S hcr. The experiments gave further support to the idea that HCR is an enzymatic process. It repairs about 80 to 90 percent of otherwise lethal UV-lesions not only in phage DNA, but also in bacterial DNA. Thehcr mutant isolated fromK12S for the purpose of this investigation, and thesyn mutant of ColiC show a very small extent of HCR; they are not completely deficient for the HCR-enzyme.

A correlation exists between the occurrence of HCR and UVR. UVR is absent in those cases where no HCR is observed. In systems with residual HCR-activity (hcr andsyn cells) UVR is less pronounced and has its maximum at lower UV-doses than in systems with full HCR-activity. UVR occurs also in unirradiated host-reactivating cells, if a large number of additional UV-lesions is introduced by means of superinfecting homologous phage. This effect is not observed in non-hostreactivating strains. The hypothesis is discussed that UVR is not a specific repair phenomenon by itself, but is the result of inhibition of cellular processes tending to decrease the survival.


Cellular Process Small Extent Enzymatic Process Specific Repair Homologous Phage 
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Copyright information

© Springer-Verlag 1963

Authors and Affiliations

  • Walter Harm
    • 1
  1. 1.Institute of GeneticsUniversity of KölnKöln-LindenthalGermany

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