Host cell reactivation of ΦX 174 at intracellular UV irradiation
If ΦX174-infected cells are UV irradiated about 3 min after the onset of phage development, host cell reactivation (HCR) of the intracellular phage can be demonstrated. HCR of ΦX174 can be observed only in host bacteria able to reactivate phages T1 and λ. Like with these two phages, HCR in ΦX174 can be inhibited by caffeine.
Since no HCR can be observed if ΦX174 is irradiated extracellularly or immediately after infection, it seems likely that only UV damage to the double stranded “replicative form” of ΦX174 can be repaired by HCR.
KeywordsCaffeine Host Cell Cell Reactivation Replicative Form Host Bacterium
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