Zeitschrift für Vererbungslehre

, Volume 94, Issue 3, pp 316–321 | Cite as

Biological melting curves for the “replicative form” of ΦX 174 DNA

  • R. Benzinger
  • P. H. Hofschneider
Article

Summary

The effect of heating and quick cooling on the infectivity of partially purified ΦX 174 replicative form was examined. A sharp increase, up to 300 fold, in infectivity was observed over a range of 4–8° C. The biologicalT M (defined as half-maximal infectivity) shows a linear dependence on the logarithm of ionic strength. The G-C content of the replicative form, as calculated from a comparison of the biologicalT M values with opticalT M values obtained byMarmur andDoty onE. coli DNA, is 45% (theoretical value=43%). The results provide a simple biological assay for the replicative form; they also indicate a high specificity of our competent bacteria for the heated replicative form.

Keywords

Ionic Strength Linear Dependence High Specificity Sharp Increase Biological Assay 

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Copyright information

© Springer-Verlag 1963

Authors and Affiliations

  • R. Benzinger
    • 1
  • P. H. Hofschneider
    • 1
  1. 1.Max-Planck-Institut für BiochemieMünchen

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