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The pattern of mutagen-induced back mutations inSalmonella typhimurium

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Summary

The effects of UV, diethyl sulfate, and 2-aminopurine in causing back mutations were studied with spontaneous and 2-aminopurine-induced leucine auxotrophs ofSalmonella typhimurium. A correlation was noted between the effectiveness of diethyl sulfate and 2-aminopurine. All the 2-aminopurine inducible mutants fell into two categories; those whose frequency of induced reversions were independent of the number of cell divisions following treatment and those whose frequencies were strongly dependent upon division. It was suggested that the two categories represented the two purine-pyrimidine base pairs.

Almost all the 2-aminopurine induced mutants belonged to the division dependent category. From this it was inferred that 2-aminopurine paired preferentially with one of the two pyrimidine bases.

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Authors and Affiliations

  1. Biological Laboratory, Cold Spring Harbor, New York, USA

    Paul Margolin & Frank H. Mukai (Postdoctoral Research Fellow, Public Health Service, National Cancer Institute)

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  1. Paul Margolin
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  2. Frank H. Mukai
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This work was supported by a research grant, RG 7178, from the Division of General Medical Sciences, Public Health Service.

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Margolin, P., Mukai, F.H. The pattern of mutagen-induced back mutations inSalmonella typhimurium . Zeitschrift für Vererbungslehre 92, 330–335 (1961). https://doi.org/10.1007/BF00890056

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  • DOI: https://doi.org/10.1007/BF00890056

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