Zeitschrift für Vererbungslehre

, Volume 98, Issue 3, pp 278–286 | Cite as

Mehrfachinfektion mit RNS-Phagen

III. Mitteilung Doppelinfektion mit fr-Mutanten und Restaurierung von f2-Mutanten mit fr-A 105
  • P. Knolle


Pre-infection of cells (grown in nutrient broth) with the fr mutant A105 resulted in partial exclusion of superinfecting fr, as determined with single burst experiments. The extent of exclusion increased with the time interval between A105- and fr-input. Pre-infection of cells (grown in yeast tryptone broth) with fr suppressed the formation of superinfecting A105, pre-infection with A105 however did not suppress the formation of superinfecting fr (determined as serum-resistant PFU). Simultaneous infection of cells, grown in yeast tryptone broth, with fr and A105 showed in single-burst experiments double infection to occur: 50% of the single bursts contained both plaque types.

Rescue of the host-dependent f2 mutants su-1 and su-3 could be demonstrated with the fr mutant A105.

With su-1, rescue could only be demonstrated if cells were simultaneously infected or if A105 preceeded su-1. Pre-infection with su-1 resulted in exclusion of A105. With su-3 the reverse was found: pre-infection with A105 resulted in exclusion of the f2 mutant, pre-infection with su-3 permitted rescue with A105. Removal of unadsorbed phages between inputs did not influence the yield of rescued phage.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brinton, C. C., P. Gemski, andJ. Carnahan: A new type of bacterial pilus genetically controlled by the fertility factor of E. coli K 12 and its role in chromosome transfer. Proc. nat. Acad. Sci. (Wash.)52, 776 (1964).Google Scholar
  2. Davern, C. I.: The inhibition and mutagenesis of an RNA bacteriophage by 5-fluorouracil. Aust. J. biol. Sci.17, 726 (1964).Google Scholar
  3. Davis, J. E., D. Pfeifer, andR. L. Sinsheimer: The replication of bacteriophage MS2, II. An in vivo specificity for the synthesis of infective RNA. J. molec. Biol.10, 1 (1964).PubMedGoogle Scholar
  4. Haruna, I., andS. Spiegelman: Specific template requirements of RNA replicases. Proc. nat. Acad. Sci. (Wash.)54, 579 (1965).Google Scholar
  5. Knolle, P.: Mehrfachinfektion mit RNS-Phagen, I. Gegenseitiger Ausschlu\ zwischen fr+ und fr-Plaquemutanten. Z. Vererbungsl.98, 180 (1966a).PubMedGoogle Scholar
  6. —: Mehrfachinfektion mit RNS-Phagen, II. Interferenz bei fr. Z. Vererbungsl.98, 270 (1966b).PubMedGoogle Scholar
  7. Knolle, P.: Faktoren, die den Plaquetest eines RNA-Phagen beeinflussen. Zbl. Bact., 1. Abt. Orig. 33–39 (1966c).Google Scholar
  8. Knolle, P.: Frühstadien der Infektion eines RNA-Phagen. Zbl. Bact., 1. Abt. Orig. (im Druck) (1966d).Google Scholar
  9. Knolle, P.: Hemmung der zelleigenen RNA-Synthese in fr-infizierten Zellen von Escherichia coli. Acta biol. med. germ. (im Druck) (1967a).Google Scholar
  10. Knolle, P.: Fr-spezifische RNA in Escherichia coli während der Phagenvermehrung. Z. Naturforsch. (zur Veröffentlichung eingereicht) (1967b).Google Scholar
  11. Lodish, H., K. Horiuchi, andN. D. Zinder: Mutants of bacteriophage f2, V. On the production of noninfectious phage particles. Virology27, 139 (1965).PubMedGoogle Scholar
  12. Notani, G. W., D. L. Engelhardt, W. Konigsberg, andN. D. Zinder: Suppression of a coat protein mutant of the bacteriophage f2. J. molec. Biol.12, 439 (1965).PubMedGoogle Scholar
  13. Scott, D. W.: Serological crossreactions among the RNA-containing coliphages. Virology26, 85 (1965).PubMedGoogle Scholar
  14. Valentine, R. C., andN. D. Zinder: Phenotypic mixing of bacteriophage f2. Virology24, 486 (1964a).PubMedGoogle Scholar
  15. —,D. L. Engelhardt, andN. D. Zinder: Host-dependent mutants of the bacteriophage f2. II. Rescue and complementation of mutants. Virology23, 159 (1964b).Google Scholar
  16. Zinder, N. D., andS. Cooper: Host-dependent mutants of the bacteriophage f2, I. Isolation and preliminary classification. Virology23, 152 (1964).PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1966

Authors and Affiliations

  • P. Knolle
    • 1
  1. 1.School of Medicine, Department of BiochemistryNew York UniversityNew YorkUSA

Personalised recommendations