Untersuchungen zur Wirkungsweise eines die UV-Empfindlichkeit bestimmenden Gens der Bakteriophagen T2 und T4
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The phage geneu, determining the difference in UV-sensitivity between T2 and T4, is shown to act by some cellular reactivation mechanism. I.e., the primary UV-lesions are identical in both phage types, but the presence of theu-allele in T4 (as opposed to theu + -allele in T2) results in reactivation of about 50% of the otherwise lethal damages. For the T4-like survival it is sufficient to have an intactu-allele within the phage-bacterium complex, but not necessarily within the proper phage to be reactivated.
The sensitivity of theu-gene, relative to the total UV-sensitivity of T2, is determined to be about (0,9±0,2)%.
Lethal UV-damages reactivable by theu-allele action are almost identical with photoreactivable damages. They do not concern preferentially genetic material with early intracellular function. At an intracellular stage, where theu-phage sensitivity is reduced to about 1/6 the original one (Luria-Latarjet-effect), theu-phage sensitivity is also reduced to 1/6.
Marker rescue experiments withu- andu + -phage led to the conclusion that the UV-damages reactivable by theu-allele action are destributed widely over a large part of the genome, with the exception of ther II-region (or at least one of the twor II-cistrons). The latter was confirmed by experiments on survival of phenotype of thisr II-cistron.
The results are discussed with respect to the extent of different kinds of UV-damages and the relations between them.
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- Adams, M. H.: Bacteriophages. Appendix: Methods of study of bacterial viruses. New York: Interscience Publ. 1959.Google Scholar
- Appleyard, R. K.: Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia coli K12. Genetics39, 440–452 (1954).Google Scholar
- Barricelli, N. A.: A “chromosomic” recombination theory for multiplicity reactivation in phages. Acta biotheor. (Leiden)11, 107–120 (1956).Google Scholar
- The partial-replica theory for phage recombination and reproduction. I. Multiplicity-reactivation and the nature of vulnerable centers. Manuskript. 1958.Google Scholar
- Benzer, S.: Resistance to ultraviolet light as an index to the reproduction of bacteriophage. J. Bact.63, 59–72 (1952).Google Scholar
- —: Fine structure of a genetic region in bacteriophage. Proc. nat. Acad. Sci. (Wash.)41, 344–354 (1955).Google Scholar
- —: The elementary units of heredity. In: A symposium on the chemical basis of heredity, p. 70–93. Baltimore: Johns Hopkins Press 1957.Google Scholar
- Doermann, A. H., M. Chase andF. W. Stahl: Genetic recombination in bacteriophage. J. cell. comp. Physiol.45, Suppl. 2, 51–74 (1955).Google Scholar
- Dulbecco, R.: Experiments on photoreactivation of bacteriophages inactivated with ultraviolet radiation. J. Bact.59, 329–347 (1950).Google Scholar
- —: A critical test of the recombination theory of multiplicity reactivation. J. Bact.63, 199–207 (1952).Google Scholar
- Epstein, R. H.: A study of multiplicity reactivation in the bacteriophage T4. I. Genetic and functional analysis of T4D-K12 (λ)-complexes. Virology6, 382–404 (1958).Google Scholar
- Garen, A., andN. D. Zinder: Radiological evidence for partial genetic homology between bacteriophage and host bacteria. Virology1, 347–376 (1955).Google Scholar
- Harm, W.: On the mechanism of multiplicity reactivation in bacteriophage. Virology2, 559–564 (1956).Google Scholar
- —: Zur Deutung der unterschiedlichen UV-Empfindlichkeit der Phagen T2 und T4. Naturwiss.45, 391–392 (1958).Google Scholar
- Krieg, D.: A study of gene action in ultraviolet-irradiated bacteriophage T4. Virology8, 80–98 (1959).Google Scholar
- Luria, S. E.: Type hybrid bacteriophages. Rec. Genet. Soc. Amer.18, 102 (1949).Google Scholar
- Luria, S. E., andR. Dulbecco: Genetic recombinations leading to production of active bacteriophage from ultraviolet inactivated bacteriophage particles. Genetics34, 93–125 (1949).Google Scholar
- Luria, S. E., andR. Latarjet: Ultraviolet irradiation of bacteriophage during intracellular growth. J. Bact.53, 149–163 (1947).Google Scholar
- Sinsheimer, R. L.: The glucose content of the desoxyribonucleic acids of certain bacteriophages. Proc. nat. Acad. Sci. (Wash.)42, 502–504 (1956).Google Scholar
- Streisinger, G.: The genetic control of ultraviolet sensitivity levels in bacteriophages T2 and T4. Virology2, 1–12 (1956).Google Scholar
- Streisinger, G., andJ. Weigle: Properties of bacteriophages T2 and T4 with unusual inheritance. Proc. nat. Acad. Sci. (Wash.)42, 504–510 (1956).Google Scholar
- Symonds, N., andE. W. McCloy: The irradiation of phage-infected bacteria: its bearing on the relationship between functional and genetic radiation damage. Virology6, 649–668 (1958).Google Scholar
- Tessman, E. S., andT. Ozaki: Multiplicity reactivation of bacteriophage T1. Virology4, 315–327 (1957).Google Scholar