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Der Einfluss des Wassergehaltes auf Inaktivierung und Mutationsrate von röntgen- und gammabestrahltenNeurospora Crassa-Conidien

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Summary

InNeurospora crassa X- and gamma-ray induced inactivation- and reversion-rates for conidia of HNO2 induced mutants were measured. With constant radiation dose inactivation rate is lowest for conidia of medium moisture content. It increases for higher and also for very low moisture content. The semilogarithmic plot of inactivation data for conidia irradiated in suspension or at medium moisture content results in a shouldered curve. A formal analysis using the number of nuclei of multinucleated conidia as numerical reference indicates that ca. 0.5 hits per nucleus are needed to inactivate such a conidium. The data for extremely dry conidia plotted from 0–210 kr likewise closely approximate a single hit curve with a steeper slope than that of the corresponding curve for conidia of medium moisture content.

Reversion rates of 5 among 7 mutants checked run parallel with inactivation with low figures for conidia of medium and increasing figures for higher as well as for very low moisture contents (class I). The reversion rate of conidia of very low moisture content of the other 2 mutants is equal or nearly equal to that of conidia of medium moisture content (class II).

Possible explanations for the data obtained are discussed.

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Authors and Affiliations

  1. Department of Biology, J. W. Gibbs Research Laboratories, Yale University, New Haven, Conn., USA

    Walter Klingmüller

  2. Max-Planck-Institut für vergleichende Erbbiologie und Erbpathologie Berlin-Dahlem, Ehrenbergstraße 26-28, 1, Berlin-Dahlem

    Walter Klingmüller

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  1. Walter Klingmüller
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Klingmüller, W. Der Einfluss des Wassergehaltes auf Inaktivierung und Mutationsrate von röntgen- und gammabestrahltenNeurospora Crassa-Conidien. Zeitschrift für Vererbungslehre 96, 116–127 (1965). https://doi.org/10.1007/BF00888783

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