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Ribonucleic acid synthesis in isolated tobacco leaf nuclei

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Summary

Nuclei isolated from tobacco leaves have been found capable of catalyzing a DNA-dependent incorporation of [8-14C] adenylic acid (supplied as the nucleoside triphosphate) into RNA in the presence of magnesium ion and the other three triphosphates.

Incorporation stops after 30 to 120 min at 28o at which time only a small fraction of the added label has been incorporated. Evidence is presented to show that this premature cessation of synthesis is due to a blocking of the DNA template by the newly synthesized RNA.

Synthesis is greatly stimulated by raising the ionic strength of the reaction mixture to 1.1, an effect believed to be due to release of molecules which normally block portions of the DNA template.

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Authors and Affiliations

  1. Abteilung Melchers, Max-Planck-Institut für Biologie, Tübingen, Germany

    Le Roy Kuehl

  2. Department of Biological Chemistry, University of Utah, Salt Lake City, Utah, USA

    Le Roy Kuehl

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  1. Le Roy Kuehl
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Kuehl, L.R. Ribonucleic acid synthesis in isolated tobacco leaf nuclei. Zeitschrift für Vererbungslehre 96, 93–104 (1965). https://doi.org/10.1007/BF00888781

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  • DOI: https://doi.org/10.1007/BF00888781

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