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Rapid effects of EGF on cytoskeletal structures and adhesive properties of highly metastatic rat mammary adenocarcinoma cells

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Abstract

In the highly metastatic rat mammary adenocarcinoma cell clone MTLn3, EGF induced increased adhesion to fibronectin while in the human epidermoid carcinoma cell line A431 EGF induced diminished adhesive properties. Flattening of cells with extensive formation of fllopodia was observed in MTLn3 cells within 5 min of EGF addition, while in A431 cells EGF induced rounding up and only occasional formation of filopodia. Immunofluorescent analysis revealed extension of microtubutes (MT) into the filopodia and Western blot analysis demonstrated an EGF-induced 2- to 3-fold increase in the amount of assembled tubulin in MTLn3 but not in A431 cells. In MTLn3, but only marginally in A431 cells, EGF treatment resulted in phosphorylation of a 280 kD cytoskeleton-associated protein, which was rapid and dose-dependent. These results suggest differential signal transduction pathways of cytoskeleton-associated EGFRs in highly metastatic MTLn3 as compared with A431 cells.

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Author notes

  1. Rosemarie B. Lichtner

    Present address: Research Laboratories of Schering AG, Mül-lerstra\e 170-178, 1000, Berlin 65, Germany

Authors and Affiliations

  1. Department of Immunology and Genetics, German Cancer Research Center, Im Neuenheimer Feld 280, 6900, Heidelberg

    Rosemarie B. Lichtner, Marion Wiedemuth & Volker Schirrmacher

  2. Research Laboratories of Schering AG, Midlerstraße 170-178, 1000, Berlin 65, Germany

    Christiane Noeske-Jungblut

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Lichtner, R.B., Wiedemuth, M., Noeske-Jungblut, C. et al. Rapid effects of EGF on cytoskeletal structures and adhesive properties of highly metastatic rat mammary adenocarcinoma cells. Clin Exp Metast 11, 113–125 (1993). https://doi.org/10.1007/BF00880072

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