Clinical & Experimental Metastasis

, Volume 11, Issue 1, pp 77–82 | Cite as

Repression of stromelysin metalloprotease expression in rat fibrosarcoma cells by dimethylsulfoxide

  • Przemyslaw Popowicz
  • Georg Engel
  • Heather Marshall
  • Stig Linder
Article

Abstract

Metalloproteases are implicated in conferring invasive properties to tumor cells. We show here that treatment of ras-oncogene-transformed rat fibroblasts with dimethylsulfoxide (DMSO) results in a reversible decrease in stromelysin mRNA. Furthermore, stromelysin expression was found to be repressed by DMSO, but not by glucocorticoid hormone, in a fibrosarcoma cell line showing low AP-1 (fos/jun) transcription factor activity. In two fibrosarcoma cell lines which express high levels of stromelysin and low levels of 68 kDa type IV collagenase, the DMSO-induced decrease in stromelysin expression was paralleled by a decreased invasive propensity.

Keywords

dimethylsulfoxide invasion metalloprotease stromelysin 

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Copyright information

© Rapid Communications of Oxford Ltd 1993

Authors and Affiliations

  • Przemyslaw Popowicz
    • 1
  • Georg Engel
    • 1
  • Heather Marshall
    • 1
  • Stig Linder
    • 1
  1. 1.Division of Experimental Oncology, Department of Oncology, RadiumhemmetKarolinska Institute and HospitalStockholmSweden

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