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Wilhelm Roux's archives of developmental biology

, Volume 191, Issue 6, pp 371–377 | Cite as

Localization of morphogenetic determinants in a special cytoplasm present in the polar lobe ofBithynia tentaculata (Gastropoda)

  • W. I. van Dam
  • M. R. Dohmen
  • N. H. Verdonk
Article

Summary

In the first polar lobe ofBithynia eggs a special plasm, the vegetal body, is present. It consists of a cupshaped aggregate of small vesicles. Centrifugation of eggs prior to first cleavage may result in displacement of the vegetal body. In about 50% of thecentriguged eggs the vegetal body is found outside the polar lobe, in one of the blastomeres. Removal of the polar lobe from non-centrifuged eggs always leads to severe defects in development. When the lobe is removed from centrifuged eggs, however, about 50% of the eggs develop into normal embryos. It is concluded that the presence of the vegetal body in a blastomere suffices to ensure normal development and, hence, that the polar lobe-specific morphogenetic determinants are contained within the vegetal body.

Key words

Morphogenetic determinants Cytoplasmic localization Polar lobe Gastropod 

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References

  1. Atkinson JW (1971) Organogenesis in normal and lobeless embryos of the marine prosobranch gastropodIlyanassa obsoleta. J Morphol 133:339–352Google Scholar
  2. Cather JN (1967) Cellular interactions in the development of the shell gland of the gastropod,Ilyanassa. J Exp Zool 166:205–224PubMedGoogle Scholar
  3. Cather JN (1971) Cellular interactions in the regulation of development in annelids and molluses. Adv Morphogen 9:67–125Google Scholar
  4. Cather JN, Verdonk NH (1974) The development ofBithynia tentaculata (Prosobranchia, Gastropoda) after removal of the polar lobe. J Embryol Exp Morphol 31:415–422PubMedGoogle Scholar
  5. Clement AC (1952) Experimental studies on germinal localization inIlyanassa. I. The role of the polar lobe in determination of the cleavage pattern and its influence in later development. J Exp Zool 121:593–625Google Scholar
  6. Clement AC (1956) Experimental studies on germinal localization inIlyanassa. II. The development of isolated blastomeres. J exp Zool 132:427–445Google Scholar
  7. Clement AC (1962) Development ofIlyanassa following removal of the D-quadrant at successive cleavage stages. J Exp Zool 149:193–215Google Scholar
  8. Clement AC (1968) Development of the vegetal half of theIlyanassa egg after removal of most of the yolk by centrifugal force, compared with the development of animal halves of similar composition. Dev Biol 17:165–186PubMedGoogle Scholar
  9. Clement AC (1971)Ilyanassa. In: Reverberi G (ed) Experimental embryology of marine and freshwater invertebrates. North-Holland, Amsterdam, pp 188–214Google Scholar
  10. Clement AC (1976) Cell determination and organogenesis in molluscan development: a reappraisal based on deletion experiments inIlyanassa. Am Zool 16:447–453Google Scholar
  11. van Dam WI, Verdonk NH (1982) The morphogenetic significance of the first quartet micromeres for the development of the snailBithynia tentaculata. Wilhelm Roux's Arch 191:112–118Google Scholar
  12. Davidson EH (1976) Gene activity in early development. 2nd ed. Academic Press, New YorkGoogle Scholar
  13. Dohmen MR, Lok D (1975) The ultrastructure of the polar lobe ofCrepidula fornicata (Gastropoda, Prosobranchia). J Embryol Exp Morphol 34:419–438PubMedGoogle Scholar
  14. Dohmen MR, Verdonk NH (1974) The structure of a morphogenetic cytoplasm, present in the polar lobe ofBithynia tentaculata (Gastropoda, Prosobranchia). J Embryol Exp Morphol 31:423–433PubMedGoogle Scholar
  15. Dohmen MR, Verdonk NH (1979) The ultrastructure and role of the polar lobe in development of molluscs. In: Subtelny S, Konigsberg IR (eds) Determinants of spatial organization. Academic Press, New York, pp 3–27Google Scholar
  16. Guerrier P, Biggelaar JAM van den, Dongen CAM van, Verdonk NH (1978) Significance of the polar lobe for the determination of dorso-ventral polarity inDentalium vulgare (da Costa). Dev Biol 63:233–242PubMedGoogle Scholar
  17. Illmensee K, Mahowald AP (1974) Transplantation of posterior pole plasm inDrosophila. Induction of germ cells at the anterior pole of the egg. Proc Natl Acad Sci USA 7:1016–1020Google Scholar
  18. Mahowald AP (1971) Polar granules ofDrosophila. IV. Cytochemical studies showing loss of RNA from polar granules during early stages of embryogenesis. J Exp Zool 176:345–352PubMedGoogle Scholar
  19. Mahowald AP (1980) Determination of germ cells inDrosophila. Eur J Cell Biol (Abstract) 22:464Google Scholar
  20. Mahowald AP, Allis CD, Karrer KM, Underwood EM, Waring GL (1979) Germ plasm and pole cells inDrosophila. In: Subtelny S, Konigsberg IR (eds) Determinants of spatial organization. Academic Press, New York, pp 127–146Google Scholar
  21. Nieuwkoop PD, Sutasurya LA (1981) Primordial germ cells in the invertebrates. Cambridge University Press, CambridgeGoogle Scholar
  22. Reynolds ES (1963) The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J Cell Biol 17:209–213Google Scholar
  23. Verdonk NH (1968) The effect of removing the polar lobe in centrifuged eggs ofDentalium. J Embryol Exp Morphol 19:33–42PubMedGoogle Scholar
  24. Verdonk NH, Cather JN (1973) the development of isolated blastomeres inBithynia tentaculata (Prosobranchia, Gastropoda). J Exp Zool 186:47–62PubMedGoogle Scholar
  25. Waring GL, Allis CD, Mahowald AP (1978) Isolation of polar granules and the identification of polar granule-specific protein. Dev Biol 66:197–206PubMedGoogle Scholar
  26. Wilson EB (1904) Experimental studies on germinal localization. I. The germ regions in the egg ofDentalium. J. Exp Zool 1:1–72Google Scholar
  27. Wilson EB (1925) The cell in heredity and development. 3rd ed. Macmillan, New YorkGoogle Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • W. I. van Dam
    • 1
  • M. R. Dohmen
    • 1
  • N. H. Verdonk
    • 1
  1. 1.Zoological LaboratoryState University of UtrechtUtrechtThe Netherlands

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