Wilhelm Roux's archives of developmental biology

, Volume 191, Issue 6, pp 371–377 | Cite as

Localization of morphogenetic determinants in a special cytoplasm present in the polar lobe ofBithynia tentaculata (Gastropoda)

  • W. I. van Dam
  • M. R. Dohmen
  • N. H. Verdonk


In the first polar lobe ofBithynia eggs a special plasm, the vegetal body, is present. It consists of a cupshaped aggregate of small vesicles. Centrifugation of eggs prior to first cleavage may result in displacement of the vegetal body. In about 50% of thecentriguged eggs the vegetal body is found outside the polar lobe, in one of the blastomeres. Removal of the polar lobe from non-centrifuged eggs always leads to severe defects in development. When the lobe is removed from centrifuged eggs, however, about 50% of the eggs develop into normal embryos. It is concluded that the presence of the vegetal body in a blastomere suffices to ensure normal development and, hence, that the polar lobe-specific morphogenetic determinants are contained within the vegetal body.

Key words

Morphogenetic determinants Cytoplasmic localization Polar lobe Gastropod 


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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • W. I. van Dam
    • 1
  • M. R. Dohmen
    • 1
  • N. H. Verdonk
    • 1
  1. 1.Zoological LaboratoryState University of UtrechtUtrechtThe Netherlands

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