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Antonie van Leeuwenhoek

, Volume 67, Issue 3, pp 255–260 | Cite as

Expression of the pCloDF13 encoded bacteriocin release protein or its stable signal peptide causes early effects on protein biosynthesis and Mg2+ transport

  • Freek Stegehuis
  • Fimme J. van der Wal
  • Joen Luirink
  • Bauke Oudega
Research Papers

Abstract

The effect of the pCloDF13 encoded bacteriocin release protein (BRP) onEscherichia coli cell lethality was studied. Induction of the BRP resulted in a strong inhibition of the incorporation of radioactive labeled amino acids and affected the transport of Mg2+ ions. Similar effects were obtained when the BRP stable signal peptide was expressed as a separate entity. Kinetic studies revealed that these effects occurred prior to quasi-lysis and release of cloacin DF13. The results indicated that the BRP induced cell lethality is caused by early effects on protein synthesis and Mg2+ transport, due to the accumulation of stable BRP signal peptides in the cytoplasmic membrane.

Key words

bacteriocin release protein culture turbidity lethality membrane transport Mg2+ uptake protein biosynthesis quasi-lysis 

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Freek Stegehuis
    • 1
  • Fimme J. van der Wal
    • 1
  • Joen Luirink
    • 1
  • Bauke Oudega
    • 1
  1. 1.Department of MicrobiologyInstitute of Molecular Biological Sciences, BioCentrum AmsterdamAmsterdamThe Netherlands

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