Abstract
A chimeric transport protein was made by expression of a fusion of thearsB genes fromEscherichia coli plasmid R773 andStaphylococcus aureus plasmid pI258. The two genes were fused to encode a functional protein with first eight membrane spanning α-helices of theS. aureus and the last four helices of theE. coli protein. The hybrid protein provided arsenite resistance and transport. When anarsA gene was expressed in trans with the ArsB proteins encoded by the R773, pI258 and fusion genes, arsenite efflux was dependent on chemical but not electrochemical energy. The Ars system is hypothesized to be a novel transport system that functions as a primary ATP-driven pump or a secondary carrier, depending on the subunit composition of the complex.
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Dou, D., Dey, S. & Rosen, B.P. A functional chimeric membrane subunit of an ion-translocating ATPase. Antonie van Leeuwenhoek 65, 359–368 (1994). https://doi.org/10.1007/BF00872219
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DOI: https://doi.org/10.1007/BF00872219