Abstract
With the aid ofrecA-lacZ fusion strains, thein vivo regulation of thePseudomonas stutzeri recA gene has been studied. It is shown that expression of this gene can be induced with a variety of DNA damaging agents, as well as with agents that interfere with DNA replication. For this induction, the presence of an active RecA protein is essential. Sequence analysis of the promoter region of theP. stutzeri recA gene showed that its open reading frame is preceded by an SOS-box, suggesting a regulation of its expression, similar to the regulation ofrecA expression inEscherichia coli.
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Abbreviations
- MMS:
-
Methyl-methane-sulphonate
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Vosman, B., Rauch, P.J.G., Westerhoff, H.V. et al. Regulation of the expression of thePseudomonas stutzeri recA gene. Antonie van Leeuwenhoek 63, 55–62 (1993). https://doi.org/10.1007/BF00871732
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DOI: https://doi.org/10.1007/BF00871732