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Dopamine receptors in the developing sheep kidney

  • Proceedings of the Third International Workshop on Developmental Renal Physiology September 6–7, 1986 Tokyo, Japan
  • Original Article
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Abstract

These studies were designed to characterize dopamine receptor density and affinity in kidneys removed from sheep of varying ages (fetal, newborn, and adult) using radioligand binding methods. Three different radioligands were used: the specific dopamine-1 antagonist3H-SCH 23390, the dopamine-1/dopamine-2 antagonist3H-haloperidol, and the dopamine-2 antagonist3H-spiroperidol. The specific binding of3H-haloperidol and3H-spiroperidol was saturable with time and ligand concentration, being indicative of dopamine receptors. The specific binding of the dopamine-1 selective radioligand3H-SCH 23390 was also saturable with time but displayed several points of saturation with increasing ligand concentration. The specific binding of3H-haloperidol, which had a low affinity and is indicative of dopamine-1 receptors, showed no age-related changes in maximum receptor density or affinity. On the other hand, the maximum receptor density of dopamine-2 receptors measured by3H-spiroperidol decreased with age. The observations that renal dopamine-1 receptor density or affinity do not change with maturation are in agreement with our pervious studies that showed no age-related changes in dopamine-receptor-mediated renal vasodilatation in sheep. The significance of the decrease in renal dopamine-2 receptor density with age remains to be determined.

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Authors and Affiliations

  1. Department of Pathology, University of Virginia Medical Center, Box 168, 22908, Charlottesville, Virginia, USA

    Robin A. Felder

  2. Department of Pediatrics, University of Iowa Hospitals and Clinics, Iowa City, Iowa, USA

    Kenneth T. Nakamura, Jean E. Robillard & Marina Kanadjian

  3. Department of Pediatrics, Georgetown University Hospital, Washington, DC, USA

    Pedro A. Jose

Authors
  1. Robin A. Felder
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  2. Kenneth T. Nakamura
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  3. Jean E. Robillard
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  4. Marina Kanadjian
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  5. Pedro A. Jose
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Felder, R.A., Nakamura, K.T., Robillard, J.E. et al. Dopamine receptors in the developing sheep kidney. Pediatr Nephrol 2, 156–162 (1988). https://doi.org/10.1007/BF00870397

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  • DOI: https://doi.org/10.1007/BF00870397

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