Summary
The autonomous synthesis of yolk proteins in ovarian follicles ofDrosophila melanogaster was analyzed. Vitellogenic follicles were labelled with35S-methionine in vitro and the newly synthesized yolk proteins were separated by SDS-polyacrylamide gel electrophoresis. Possible contamination of the follicle preparations caused by adhering fat body cells could be excluded by culturing follicles in males prior to labelling in vitro. When labelled follicles were cut at the nurse cell/oocyte border the three yolk proteins (YP1, YP2, YP3) were found only in posterior fragments containing ooplasm and follicle cells, whereas two radioactive protein bands (A and B) were detected in nurse cells (anterior fragments). The yolk proteins of these five bands were characterized by peptide mapping. Band A protein, migrating a little more slowly than YP2, is closely related to both YP1 and YP2 while band B contains a yolk protein which is very similar to YP3. Hence, the nurse cells have been identified as a site of vitellogenin synthesis within the ovary ofDrosophila.
References
Bonner WM, Laskey RA (1974) A film detection method for Tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem 46:83–88
Bownes M (1979) Three genes for three yolk proteins inDrosophila melanogaster. FEBS Lett 100:95–98
Bownes M (1980) The use of yolk protein variations inDrosophila species to analyze the control of vitellogenesis. Differentiation 16:109–116
Bownes M, Hames BD (1977) Accumulation and degradation of three major yolk proteins inDrosophila melanogaster. J Exp Zool 200:149–155
Bownes M, Hames BD (1978) Analysis of the yolk proteins inDrosophila melanogaster. FEBS Lett 96:327–330
Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252:1102–1106
Gavin JA, Williamson JH (1976) Synthesis and deposition of yolk protein in adultDrosophila melanogaster. J Insect Physiol 22:1457–1464
Gelti-Douka H, Gingeras TR, Kambysellis MP (1974) Yolk proteins inDrosophila: Identification and site of synthesis. J Exp Zool 187:167–172
Gutzeit HO, Gehring WJ (1979) Localized synthesis of specific proteins during oogenesis and early embryogenesis inDrosophila melanogaster. Wilhelm Roux's Archives 187:255–266
Kambysellis MP (1977) Genetic and hormonal regulation of vitellogenesis inDrosophila. Am Zool 17:535–549
King RC (1970) Ovarian development inDrosophila melanogaster Academic Press, New York, London
Robb JA (1969) Maintanance of imaginal discs ofDrosophila melanogaster in chemically defined media. J Cell Biol 41:876–885
Srdić Ž, Reinhardt C, Beck H, Gloor H (1979) Autonomous yolk protein synthesis in ovaries ofDrosophila cultured in vivo. Wilhelm Roux's Archives 187:255–266
Warren TG, Mahowald AP (1979) Isolation and partial chemical characterization of the three major yolk polypeptides fromDrosophila melanogaster. Dev Biol 68:130–139
Warren TG, Brennan MD, Mahowald AP (1979) Two steps in maturation of vitellogenin polypeptides inDrosophila melanogaster. Proc Natl Acad Sci 76:2848–2852
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Supported by the Deutsche Forschungsgemeinschaft, SFB 46
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Gutzeit, H.O. Yolk synthesis in ovarian follicles ofDrosophila . Wilhelm Roux' Archiv 189, 221–224 (1980). https://doi.org/10.1007/BF00868681
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DOI: https://doi.org/10.1007/BF00868681