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Oxidative damage of red blood cells in haemolytic uraemic syndrome

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Abstract

Changes in red blood cell (RBC) lipid peroxidation [measured by malonyl dialdehyde (MDA) concentration], glutathione (GSH) metabolism, antioxidant enzyme activities (catalase, superoxide dismutase, glutathione peroxidase) and haemoglobin (Hb) metabolites (metHb, carboxy Hb) were studied in six children with post-enteropathic (D+) haemolytic uraemic syndrome (HUS) and ten controls. The in vitro effect of hydrogen peroxide [acetyl-phenylhydrazine (APH) test] on GSH and Hb metabolism was also investigated. MDA levels were significantly higher and the antioxidant enzyme activities were lower in HUS patients than in the controls (P<0.01). The oxidised glutathione concentration was significantly higher in the patients than in the control children (26.3±12.6 vs. 10.9±1.8 nmol/g Hb. Percentage values of carboxy Hb and metHb were also higher in HUS (P<0.01). Incubation of RBC with APH induced a more pronounced decrease in the concentration of GSH (P<0.001) and a significant increase (P<0.01) in the level of metHb and carboxy Hb in the HUS patients. This suggests that there is reduced RBC GSH stability in HUS. Utilisation of GSH and antioxidant enzymes leads to increased Hb oxidation and haemolysis. The oxidative damage may have an important role in the pathogenesis of haemolytic anaemia in HUS.

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References

  1. Nafstad I (1974) Endothelial damage and platelet thrombosis associated with PUFA-rich, vitamin E deficient diet fed to pig. Thromb Res 5:251–255

    Google Scholar 

  2. Teige J, Nordstoga K, Fjolstad M, Nafstad I (1973) The generalized Schwartzman reaction in pigs induced by diet and single injection of endotoxin fromEscherichia coli. Acta Vet Scand 14:92–106

    PubMed  Google Scholar 

  3. Kaplan BS, Mills M (1988) Elevated serum elastase and alpha-1-antitrypsin levels in hemolytic uremic syndrome. Clin Nephrol 30:193–196

    PubMed  Google Scholar 

  4. Milford D, Taylor CM, Rafaat F, Halloran E, Dawes J (1989) Neutrophil elastases and haemolytic uraemic syndrome (letter). Lancet 2:1153

    Google Scholar 

  5. Fitzpatrick MM, Shah V, Filler G, Dillon MJ, Barratt TM (1992) Neutrophil activation in the haemolytic uraemic syndrome: free and complexed elastase in plasma. Pediatr Nephrol 6:50–53

    PubMed  Google Scholar 

  6. Neild G (1992) Haemolytic uraemic syndrome (including disseminated intravascular coagulation and thrombotic thrombocytopenic purpura), In: Cameron S, Davison AM, Grunfeld J-P, Kerr D, Ritz E (eds) Oxford textbook of clinical nephrology. Oxford University Press, Oxford, pp 1041–1060

    Google Scholar 

  7. Forsyth KD, Simpson AC, Fitzpatrick MM, Barratt TM, Levinsky RJ (1989) Neutrophil-mediated endothelial injury in haemolytic uraemic syndrome. Lancet 2:411–414

    PubMed  Google Scholar 

  8. Walters MD, Matthei IU, Kay R, Dillon MJ, Barratt TM (1989) The polymorphonuclear leucocyte count in childhood haemolytic uraemic syndrome. Pediatr Nephrol 3:130–134

    PubMed  Google Scholar 

  9. Tietze F (1969) Enzymic method for quantitative deterioration of nanogram amounts of total and oxidized glutathione: application to mammalian blood and other tissues. Anal Biochem 27:502–522

    PubMed  Google Scholar 

  10. Akerboom TPM, Sies H (1981) Assay of glutathione, glutathione, disulphide and glutathione mixed disulphides in biological samples. Methods Enzymol 77:373–382

    PubMed  Google Scholar 

  11. Guntherberg H, Rapaport S (1968) Eine Methode zur Bestimmung des oxydierten Glutathions. Acta Biol Med Germ 20:559–564

    PubMed  Google Scholar 

  12. Beutler E (1957) The glutathione instability of drug-sensitive red cells. A new method for the in vitro detection of drug sensitivity. J Lab Clin Med 49:84–95

    PubMed  Google Scholar 

  13. Matkovics B, Novak Z, Hoang DH, Szabo L, Vargha SJ, Zalesna G (1987) A comparative study on some important experimental animal peroxide metabolism enzymes. Comp Biochem Physiol [β] 56:31–34

    Google Scholar 

  14. Misra HP, Fridovich I (1972) The role of superoxide anion in the antioxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175

    PubMed  Google Scholar 

  15. Sedlak I, Lindsay RH (1968) Estimation of total, protein-bound and nonprotein sulphydryl groups in tissue with Ellman's reagent. Anal Biochem 25:192–205

    PubMed  Google Scholar 

  16. Chin DTY, Stults FH, Tappal AL (1976) Purification and properties of rat lung soluble glutathione peroxidase. Biochim Biophys Acta 445:558–566

    PubMed  Google Scholar 

  17. Placer ZA, Cusman L, Johnson BC (1966) Estimation of product of lipid peroxidation by malonyl dialdehyde in biochemical systems. Anal Biochem 16:359–364

    PubMed  Google Scholar 

  18. Turi S, Nemeth I, Vargha I, Matkovics B, Dobos E (1991) Erythrocyte defence mechanisms against free oxygen radicals in haemodialysed uraemic children. Pediatr Nephrol 5:179–183

    PubMed  Google Scholar 

  19. Lowry OH, Rosebrough NI, Farr AL, Randal R (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    PubMed  Google Scholar 

  20. Brown RE, Alade SL, Knight JA, Evans BJ (1988) Serum lipoperoxidation products in an infant with hemolytic-uremic syndrome. Clin Chem 34:2382–2384

    PubMed  Google Scholar 

  21. Situnayake RD, Crump NJ, Turnham DI, Taylor CM (1991) Further evidence of lipid peroxidation in post-enteropathic haemolytic-uraemic syndrome. Pediatr Nephrol 5:387–392

    PubMed  Google Scholar 

  22. O'Regan S, Chesney RW, Kaplan BS, Drummond KN (1981) Red cell membrane phospholipid abnormalities in the hemolytic uremic syndrome. Clin Nephrol 15:14–17

    PubMed  Google Scholar 

  23. Cordes EH, Jencks WP (1962) On the mechanism of Schiff base formation and hydrolysis. J Am Chem Soc 84:832–837

    Google Scholar 

  24. Knight JA, Smith SE, Kinder VE, Anstall HB (1987) Reference intervals for plasma lipoperoxides: age-, sex-, and specimen-related variations. Clin Chem 33:2289–2291

    PubMed  Google Scholar 

  25. Janero DR (1990) Malondialdehyde and thiobarbituric acid reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med 9:515–540

    PubMed  Google Scholar 

  26. Hochstein P, Jain SR (1981) Association of lipid peroxidation and polymerization of membrane proteins with erythrocyte aging. Physiological significance of lipid peroxidation. Fed Proc 40:183–188

    PubMed  Google Scholar 

  27. Boca Raton (1985) Pathological states. In: Oberley LW (ed) Superoxide dismutase, vol 3. CRC Press, New York, pp 151–236

    Google Scholar 

  28. Powell HR, McCredie DA, Taylor CM, Burke JR, Walker RG (1984) Vitamin E treatment of haemolytic uraemic syndrome. Arch Dis Child 59:401–404

    PubMed  Google Scholar 

  29. Aggarwal SK, Saini AS (1984) Formation and detoxification of oxygen free radicals and peroxides in erythrocytes. Indian J Haematol 2:39–44

    Google Scholar 

  30. Costagliola C, Libondi T, Menzione M, Rinaldi E, Aurichio G (1985) Vitamin E and red blood cell glutathione. Metabolism 34:712–714

    PubMed  Google Scholar 

  31. Costagliola C, Menzione M, Romano L (1987) Oxidative state of glutathione in arterial and venous red blood cells and plasma of rabbit. Eur J Haematol 38:327–330

    PubMed  Google Scholar 

  32. Jocelyn PC (1972) Biochemistry of the SH groups. Academic Press, London, p 247

    Google Scholar 

  33. Eggleston LV, Krebs HA (1974) Regulation of the pentose phosphate cycle. Biochem J 138:425–435

    PubMed  Google Scholar 

  34. Szebeni J, Winterbourn CC, Carrell RW (1984) Oxidative interactions between haemoglobin and membrane lipid. A liposome model. Biochem J 220:685–692

    PubMed  Google Scholar 

  35. Longo LD (1977) The biological effects of carbon monoxide on the pregnant woman, fetus and newborn infant. Am J Obstet Gynecol 129:59–102

    Google Scholar 

  36. Bolande PP, Kaplan BS (1985) Experimental studies on the hemolytic-uremic syndrome. Nephron 39:228–236

    PubMed  Google Scholar 

  37. Moncada S, Gryglewski RJ, Bunting S, Vane JR (1976) A lipid peroxide inhibits the enzyme in blood vessel microsomes that generates from prostaglandin endoperoxides the substance (prostaglandin X) which prevents platelet aggregation. Prostaglandins 12: 715–737

    PubMed  Google Scholar 

  38. Túri S, Beattie TJ, Belch JJF, Murphy AV (1986) Disturbances of prostacyclin metabolism in children with haemolytic uraemic syndrome and in first degree relatives. Clin Nephrol 25:193–198

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Paediatrics, Albert Szent-Györgyi Medical University, 14 Korányi Street, H-6725, Szeged, Hungary

    Sándor Túri & Ilona Németh

  2. Department of Biology, Attila József University, H-6725, Szeged, Hungary

    Ilona Vargha & Béla Matkovics

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  1. Sándor Túri
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  2. Ilona Németh
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  3. Ilona Vargha
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  4. Béla Matkovics
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Túri, S., Németh, I., Vargha, I. et al. Oxidative damage of red blood cells in haemolytic uraemic syndrome. Pediatr Nephrol 8, 26–29 (1994). https://doi.org/10.1007/BF00868253

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  • DOI: https://doi.org/10.1007/BF00868253

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