Summary
Deficient limb buds composed of prospective stylopod and autopod are able to regulate the missing intercalary zeugopod, the origin of which was investigated by heterospecific quail/chick recombinants. The associations of quail prospective autopod and chick prospective stylopod failed to regulate. The reverse combination of chick prospective autopod grafted onto a quail prospective stylopod gave rise to a three-segmented limb. In 13 out of 16 cases the regulated zeugopod was made up of both chick and quail cells. Chick cells were located predominantly along the postaxial half of the zeugopod, while the quail cells made up most of its preaxial half. In two cases, the intercalary zeugopod consisted exclusively of chick cells originating from the tip and in one case of quail cells originating from the base.
These results demonstrate that during the regulative processes, the prospective values of some of the original stylopodial and autopodial cells have been shifted along the proximo-distal axis, towards the expression of more distal as well as of more proximal structures.
Heteropolar stylo-autopodial or zeugo-autopodial recombinants, in which the proximo-distal axis of the base was reversed with respect to that of the tip, were unable to regulate the pattern defects and thus revealed the importance of concordant p-d polarity for regulative processes to take place between abutted tissues.
Similar content being viewed by others
References
Amprino, R., Camosso, M.: Analisi sperimentale dello sviluppo dell' ala nell' embrione di pollo. Wilhelm Roux's Archives150, 509–541 (1958)
Feulgen, R., Rossenbeck, H.: Mikroskopisch-chemischer Nachweis einer Nucleinsäure vom Typus der Thymonucleinsäure und die darauf beruhende elektive Färbung von Zellkernen in mikroskopischen Präparaten. Z. Physiol. Chem.135, 203–252 (1924)
Hamburger, V., Hamilton, H. L.: A series of normal stages in the development of the chick embryo. J. Morph.88, 49–92 (1951)
Hampe, A.: Contribution à l'étude du développement et de la régulation des déficiences et des excédents dans la patte de l'embryon de poulet. Arch. Anat. Micr. Morph. Exp.48, 345–478 (1959)
Iten, L. E., Bryant, S.: The interaction between the blastema and stump in the establishment of the anterior-posterior and proximo-distal organization of the limb regenerate. Develop. Biol.44, 119–147 (1975)
Kieny, M.: Rôle inducteur du mésoderme dans la différenciation précoce du bourgeon de membre chez l'embryon de Poulet. J. Embryol. Exp. Morph.8, 457–467 (1960)
Kieny, M.: Régulation des excédents et des déficiences du bourgeon d'aile de l'embryon de Poulet. Arch. Anat. Micr. Morph. Exp.53, 29–44 (1964a)
Kieny, M.: Etude du mécanisme de la régulation dans le développement du bourgeon de membre de l'embryon de Poulet. II. Régulation des déficiences dans les chimères “aile-patte” et “patteaile”. J. Embryol. Exp. Morph.12, 357–371 (1964b)
Kieny, M., Pautou, M. P.: Régulation des excédents dans le développement du bourgeon de membre de l'embryon d'oiseau. Analyse expérimentale de combinaisons xénoplastiques caille/poulet. Wilhelm Roux's Archives179, 327–338 (1976)
Le Douarin, N., Barq, G.: Sur l'utilisation des cellules de caille japonaise comme “marqueurs biologiques” en embryologie expérimentale. Paris: C.R. Acad. Sci.269, 1543–1546 (1969)
Lundvall, H.: Färbung des Skelettes in durchsichtigen Weichteilen. Anat. Anz.62, 353–378 (1927)
Pautou, M. P.: Analyse de la morphogenèse du pied des Oiseaux à l'aide de mélanges cellulaires interspécifiques. I. Etude morphologique. J. Embryol. Exp. Morph.29, 175–196 (1973)
Saunders, J. W. Jr., Cairns, J. M., Gasseling, M. T.: The role of the apical ectodermal ridge of ectoderm in the differentiation of the morphological structure and inductive specificity of limb parts in the chick. J. Morph.101, 57–87 (1957)
Stocum, D. L.: Regulation after proximal or distal transposition of limb regeneration blastomas and determination of the proximal boundary of the regenerate. Develop. Biol.45, 112–136 (1975)
Summerbell, D., Lewis, J. H., Wolpert, L.: Positional information in chick limb morphogenesis. Nature (London)244, 492–496 (1973)
Wolff, E., Hampe, A.: Sur la régulation de la patte de Poulet après résection d'un segment intermédiaire. Paris: C.R. Soc. Biol.148, 154–156 (1954)
Wolpert, L., Lewis, J., Summerbell, D.: Morphogenesis of the vertebrate limb. In “Cell patterning”. 29th Ciba Foundation Symposium, pp. 95–130 (1975)
Zwilling, E.: Interaction between limb-bud ectoderm and mesoderm in the chick embryo. II. Experimental limb duplication. J. Exp. Zool.132, 173–187 (1956)
Zwilling, E.: Limb morphogenesis. Adv. Morph.1, 301–330 (1961)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kieny, M., Pautou, MP. Proximo-distal pattern regulation in deficient avian limb buds. Wilhelm Roux' Archiv 183, 177–191 (1977). https://doi.org/10.1007/BF00867319
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00867319