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Studies on renal adaptation to altered dietary amino acid intake: reduced renal cortex taurine content increases the Vmax of taurine uptake by brush border membrane vesicles

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Abstract

Rats were placed on a normal taurine diet (NTD), low taurine diet (LTD) or a high taurine diet (HTD) for 14 days. β-Alanine was fed to half of the animals in each group and resulted in a lowered renal cortex taurine content. Brush border membrane vesicle (BBMV) uptake of taurine was higher after β-alanine feeding and was associated with an increase in Vmax of uptake. β-Alanine feeding to HTD animals also altered the Km of uptake, possibly since the load of sulfur amino acids (6% of diet) was high. As a control, glycine (3%) feeding for 8 days along with each diet did not alter the plasma or renal cortex content; BBMV uptake as well as Km and Vmax of taurine accumulation were minimally altered. Accordingly, ingestion of a non-sulfur-containing α-amino acid did not change β-amino acid transport. This study provides evidence that whole body taurine homeostasis is maintained in the presence of a taurine-depleting agent (β-alanine feeding) by an increase in the number of Na+-taurine uptake sites.

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Author information

Author notes

  1. Shermine Dabbagh

    Present address: Children's Hospital, Pittsburg, Pennsylvania, USA

Authors and Affiliations

  1. Department of Pediatrics, University of California, Davis, School of Medicine, Davis, Calif., USA

    Russell W. Chesney

  2. Pediatric Renal Disease Laboratory, University of Wisconsin, Clinical Sciences Center, Madison, Wiscon, USA

    Naomi Gusowski & Shermine Dabbagh

Authors
  1. Russell W. Chesney
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  2. Naomi Gusowski
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  3. Shermine Dabbagh
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Additional information

Supported in part by grants from NIH RO1 AM 31682-02, AM 37223-01 and the Medical School and Graduate School Research Committees of The University of Wisconsin. Dr. Dabbagh is supported by the Pearl M. Stetler Foundation. Dr. Chesney was the recipient of a Research Career Development Award KO4 AM 00421.

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Chesney, R.W., Gusowski, N. & Dabbagh, S. Studies on renal adaptation to altered dietary amino acid intake: reduced renal cortex taurine content increases the Vmax of taurine uptake by brush border membrane vesicles. Pediatr Nephrol 1, 9–15 (1987). https://doi.org/10.1007/BF00866878

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  • DOI: https://doi.org/10.1007/BF00866878

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