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Interleukin-6 and interleukin-8 in the urine of children with acute pyelonephritis

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Abstract

Interleukin-6 (IL-6) and interleukin-8 (IL-8) are important mediators of the inflammatory response in serious bacterial infections. We studied the levels of these two cytokines (standardised for urinary creatinine) in the urine of infants and children during and 6 weeks after acute pyelonephritis and in non-renal febrile controls and healthy children without apparent infection. IL-6 was detected in the urine of 52% of children with pyelonephritis compared with 15% of other children (P<0.001). The median urinary IL-6 level in acute pyelonephritis was 4 pg/μmol compared with undetectable levels in the control group (P<0.001). IL-8 was detected in 98% of children with pyelonephritis and 42% of other children (P<0.001). The median concentration of IL-8 was 188 pg/μmol in pyelonephritis; it was undetectable in controls (P<0.001). IL-8 levels were higher in children less than 1 year of age (P<0.001).

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References

  1. Winberg J, Andersen HJ, Bergström T, Jacobsson B, Larson H, Lincoln K (1974) Epidemiology of symptomatic urinary tract infection in childhood. Acta Paediatr Scand [Suppl] 252: 1–20

    Google Scholar 

  2. Donckerwolke RA, Broyer M, Brunner FP, Brynger H, Jacobs C, Kramer P, Selwood NH, Wing AJ (1983) Combined report on regular dialysis and transplantation in Europe. XI. 1981. Proc Eur Dial Transplant Assoc 19: 61–91

    PubMed  Google Scholar 

  3. Calandra T, Gerain J, Heumann D, Baumgartner J-D, Glauser MP and the Swiss J5 immunoglobulin study group (1991) High circulating levels of interleukin-6 in patients with septic shockevolution during sepsis, prognostic value, and interplay with other cytokines. Am J Med 91: 23–29

    PubMed  Google Scholar 

  4. Sullivan JS, Kilpatrick L, Costarino AT Jr, Lee SC, Harris MC (1992) Correlation of plasma cytokine elevations with mortality rate in children with sepsis. J Pediatr 120: 510–515

    PubMed  Google Scholar 

  5. Groll AH, Meiser A, Weise M, Rettwitz-Volk W, Loewenich V von, Gussetis ES, Kornhuber B (1992) Interleukin 6 as early mediator in neonatal sepsis. Pediatr Infect Dis J 11: 496–498

    PubMed  Google Scholar 

  6. Rusconi F, Parizzi F, Garlaschi L, Assael BM, Sironi M, Ghezzi P, Mantovani A and the colloborative study on meningitis (1991) Interleukin 6 activity in infants and children with bacterial meningitis. Pediatr Infect Dis J 10: 117–121

    PubMed  Google Scholar 

  7. Hedges S, Stenqvist K, Lidin-Janson G, Martinell J, Sandberg T, Svanborg C (1992) Comparison of urine and serum concentrations of interleukin-6 in women with acute pyelonephritis or asymptomatic bacteriuria. J Infect Dis 166: 653–656

    PubMed  Google Scholar 

  8. Ohta K, Takano N, Seno A, Yachie A, Miyawaki T, Yokohama H, Tomosugi N, Kato E, Taniguchi N (1992) Detection and clinical usefulness of urinary interleukin-6 in the diseases of the kidney and urinary tract. Clin Nephrol 38: 185–189

    PubMed  Google Scholar 

  9. Hedges S, Anderson P, Lidin-Janson G, De Man P, Svanborg C (1991) Interleukin-6 response to deliberate colonization of the human urinary tract with gram-negative bacteria. Infect Immun 59: 421–427

    PubMed  Google Scholar 

  10. Hack CE, Hart M, Strack Van Schijndel RJM, Eerenberg AJM, Nuilens JH, Thijs LG, Aarden LA (1992) Interleukin-8 in sepsis: relation to shock and inflammatory mediators. Infect Immun 60: 2835–2842

    PubMed  Google Scholar 

  11. Redl H, Schlag G, Bahrani S, Schade U, Ceska M, Stûtz P (1991) Plasma neutrophil-activating peptide-1/interleukin-8 and neutrophil elastase in a primate bacteremia model. J Infect Dis 164: 383–388

    PubMed  Google Scholar 

  12. Van Zee KJ, Deforge LE, Fischer E, Marano MA, Kenney JS, Remick DG, Lowry SF, Moldawer LL (1991) IL-8 in septic shock, endotoxemia, and after IL-1 administration. J Immunol 146: 3478–3482

    PubMed  Google Scholar 

  13. Gauldie J, Richards C, Harnish D, Lansdorp P, Baumann H (1987) Interferon beta2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulatory factor and regulates the major acute phase response in liver cells. Proc Natl Acad Sci USA 84: 7251–7255

    PubMed  Google Scholar 

  14. Helle M, Brakenhoff JPJ, Groot ER de, Aarden LA (1988) Interleukin 6 is involved in interleukin-1 induced activities. Eur J Immunol 18: 957–959

    Google Scholar 

  15. Larsen Gronhoj C, Andersen AO, Appella E, Oppenheim JJ, Matsushima K (1989) The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes. Science 243: 1464–1466

    PubMed  Google Scholar 

  16. Rampart M, Van Damme J, Zonnekeyn L, Herman AG (1989) Granulocyte chemotactic protein/interleukin-8 induces plasma leakage and neutrophil accumulation in rabbit skin. Am J Pathol 135: 21–25

    PubMed  Google Scholar 

  17. Peveri P, Walz A, Dewald B, Baggiolini M (1988) A novel neutrophil-activating factor produced by human mononuclear phagocytes. J Exp Med 167: 1547–1559

    PubMed  Google Scholar 

  18. Masson P, Ohlsson P, Björkhem J (1981) Combined enzymic-Jaffé method for determination of creatinine in serum. Clin Chem 27: 18–21

    PubMed  Google Scholar 

  19. Rugo HS, O'Hanley P, Bishop AG, Pearce MK, Abrams JS, Howard M, O'Garra A (1992) Local cytokine production in a murine model ofEscherichia coli pyelonephritis. J Clin Invest 89: 1032–1039

    Google Scholar 

  20. Glauser MP, Meylan P, Billie J (1987) The inflammatory response and tissue damage. Pediatr Nephrol 1: 615–622

    PubMed  Google Scholar 

  21. Matsumoto T, Mizunoe Y, Sakamoto N, Kumazawa J (1990) Suitability of colchicine and superoxide dismutase for the suppression of renal scarring following an infection with bacteria showing mannose-sensitive pilf. Nephrol 56: 130–135

    Google Scholar 

  22. Man P de, Jodal U, Lincoln K, Svanborg Edén C (1988) Bacterial attachment and inflammation in the urinary tract. J Infect Dis 158: 29–35

    PubMed  Google Scholar 

  23. Man P de, Claeson I, Johanson IM, Jodal U, Svanborg Edén C (1989) Bacterial attachment as a predictor of renal abnormalities in boys with urinary tract infection. J Pediatr 115: 915–922

    PubMed  Google Scholar 

  24. Hedges S, Svensson M, Svanborg C (1992) Interleukin-6 response of epithelial cell lines to bacterial stimulation in vitro. Infect Immun 60: 1295–1301

    Google Scholar 

  25. Schmouder RL, Strieter RM, Wiggins RC, Chensue SW, Kunkel SL (1992) In vitro and in vivo interleukin-8 production in human renal cortical epithelia. Kidney Int 41: 191–198

    PubMed  Google Scholar 

  26. Abbott F, Ryan JJ, Ceska M, Matsushima K, Sarraf CE, Rees AJ (1991) Interleukin-lbeta stimulates human mesangial cells to synthesize and release interleukin-6 and interleukin-8. Kidney Int 40: 597–605

    PubMed  Google Scholar 

  27. Brown Z, Strieter RM, Chensue SW, Ceska M, Lindley I, Neild GH, Kunkel SL, Westwick J (1991) Cytokine-activated human mesangial cells generate the neutrophil chemoattractant, interleukin 8. Kidney Int 40: 86–90

    PubMed  Google Scholar 

  28. Linder H, Engberg I, Van Kooten C, De Man P, Svanborg-Edén C (1990) Effects of anti-inflammatory agents on mucosal inflammation induced by infection with Gram-negative bacteria. Infect Immun 58: 2056–2060

    PubMed  Google Scholar 

  29. Mustafa MM, Ramilo O, Saez-Llorenz X, Olsen KD, Magness RR, McCracken GH Jr (1990) Cerebrospinal fluid prostaglandins, interleukin lbeta, and tumor necrosis factor in bacterial meningitis. Clinical and laboratory correlations in placebo-treated and dexamethasone-treated patients. Am J Dis Child 144: 883–887

    PubMed  Google Scholar 

  30. Lebel MH, Freij BJ, Syrogiannopoulos GA, Chrane DF, Hoyt MJ, Stewart SM, Kennert BD, Olsen KD, McCracken GH Jr (1988) Dexamethasone therapy for bacterial meningitis. Results of two double-blind, placebo-controlled trials. N Engl J Med 319: 964–971

    PubMed  Google Scholar 

  31. Kennedy WA, Hoyt MJ, McCracken GH Jr (1991) The role of corticosteroid therapy in children with pneumococcal meningitis. Am J Dis Child 145: 1374–1378

    PubMed  Google Scholar 

  32. Ohlsson K, Björk P, Bergenfeldt M, Hageman R, Thompson RC (1990) Interleukin-1 receptor antagonist reduces moitality from endotoxin shock. Nature 348: 550–552

    PubMed  Google Scholar 

  33. Wakabayashi G, Gelfand JA, Burke JF, Thompson RC, Dinarello CA (1991) A specific receptor antagonist for interleukin 1 preventsEscherichia coli-induced shock in rabbits. FASEB J 5: 338–343

    PubMed  Google Scholar 

  34. Fischer E, Marano MA, Van Zee KJ, Rock CS, Hawes AS, Thompson WA, Deforge L, Kenney JS, Remick DG, Bloedow DC, Thompson RC, Lowry SF, Moldawer LL (1992) Inteneukin-1 receptor blockade improves survival and hemodynamic performance inEscherichia coli septic shock, but fails to alter host responses to sublethal endotoxemia. J Clin Invest 89: 1551–1557

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Paediatrics, St. Göran's Children's Hospital, S-112 81, Stockholm, Sweden

    Kjell Tullus & Omar Fituri

  2. Department of Bacteriology, Nauonal Bacteriological Laboratory, S-105 21, Stockholm, Sweden

    Lars G. Burman

  3. Danderyd Hospital, S-182 88, Danderyd, Sweden

    Bengt Wretlind

  4. Stockholm County Council Central Microbiological Laboratory, Box 70470, S-107 26, Stockholm, Sweden

    Annelie Brauner

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  1. Kjell Tullus
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  2. Omar Fituri
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  4. Bengt Wretlind
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  5. Annelie Brauner
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Tullus, K., Fituri, O., Burman, L.G. et al. Interleukin-6 and interleukin-8 in the urine of children with acute pyelonephritis. Pediatr Nephrol 8, 280–284 (1994). https://doi.org/10.1007/BF00866334

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  • DOI: https://doi.org/10.1007/BF00866334

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