The interaction of 4′, 6-diamidino-2-phenylindole (DAPI) with pepsin
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We recently found that the fluorescent dye DAPI, well-known for its use with nucleic acids, is also able to interact with proteins as well as ordered phospholipids assemblies. The interaction of DAPI with pepsin under different conditions of pH and ionic strength was studied with fluorescence and circular dichroism techniques. From a comparison of the results obtained, the interaction appears to be rather tight and specific, dependent on both electrostatic and hydrophobic forces, and able to probe the tridimensional conformation of the protein.
Key Words4′, 6-Diamidino-2-phenylindole (DAPI) pepsin, fluorescence, circular dichroism, binding
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