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Journal of Fluorescence

, Volume 1, Issue 4, pp 225–234 | Cite as

Fluorescence anisotropy of cyanobacterial phycobilisomes oriented in polyvinyl alcohol (PVA) films

  • D. Frąckowiak
  • A. Dudkowiak
  • B. Zelent
  • R. M. Leblanc
Article

Abstract

Polarized absorption (at 296 and 85 K), fluorescence, and photoacoustic (at 296 and 85 K) spectra of antenna complexes—phycobilisomes isolated from cyanobacteriaTolypothrix tenuis andOscillatoria and embedded in isotropic and anisotropic polyvinyl alcohol films—were measured. From the sets of polarized components of emission, the anisotropy of fluorescence for the pools of differently oriented molecules was calculated. On the basis of polarized photoacoustic and emission spectra, the competition between the process of thermal deactivation of excitation and excitation energy transfer in a chain of excitation donor and acceptor chromophores of phycobilisomes is discussed.

Key Words

Cyanobacteria excitation energy transfer photoacoustic spectra phycobilisomes polarized fluorescence polyvinyl alcohol (PVA) films 

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Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • D. Frąckowiak
    • 1
  • A. Dudkowiak
    • 1
    • 2
  • B. Zelent
    • 1
  • R. M. Leblanc
    • 1
  1. 1.Centre de recherche en photobiophysiqueUniversité du Québec à Trois-RivièresTrois-RivièresCanada
  2. 2.Institute of PhysicsPoznań Technical UniversityPoznańPoland

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