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Nuclear nonhistone proteins in mouse teratocarcinomas

I. Cell lineage specificity

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Summary

The nonhistone protein pattern of four murine teratocarcinomas with different capacities for differentiation were compared: a multidifferentiated teratocarcinoma OTT2289, a nondifferentiated teratocarcinoma OTT2158, a teratocarcinoma-derived rhabdomyosarcoma TDR114, and a teratocarcinoma-derived neuroblastoma TDN2151. Their nonhistone proteins (NHP) were separated by differential salt extraction and hydroxyapatite chromatography into three fractions, NHP-I, NHP-II and NHP-III. Comparison of the NHP fractions by twodimensional gel electrophoresis in combination with a sensitive silver staining method reveals that there are several tumour line specific proteins in each NHP fraction. We suggest that specific NHP, which can be used as biochemical markers for each of the four investigated tumour lines, may be involved in cell lineage specific control of gene expression.

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References

  • Artzt K, Jacobs-Cohen RJ, DiMeo A, Alton AK, Darlington G (1981) Reexpression of a T/t-complex antigen (t12) in thymocytes x embryonal carcinoma cell hybrids. Somatic Cell Genet 7:423–434

    PubMed  Google Scholar 

  • Blüthmann H (1978) Two DNA-binding nonhistone chromsomal proteins from mouse myeloma tumor cells. Int J Biochem 9:469–476

    PubMed  Google Scholar 

  • Blüthmann H, Mrozek S, Gierer A (1975) Non-histone chromsomal proteins. Their isolation and role in determining specificity of transcription in vitro. Eur J Biochem 58:315–326

    PubMed  Google Scholar 

  • Blüthmann H, Illmensee K (1981) Nonhistone proteins in mouse teratocarcinomas. Experientia 37:638

    Google Scholar 

  • Carter DB, Harris SE (1979) Chromosomal proteins from the embryoid body/teratocarcinoma system. Dev Biol 69:1–14

    Google Scholar 

  • Comings DE, Harris DC (1976) Nuclear proteins. II. Similarity of nonhistone proteins in nuclear sap and chromatin, and essential absence of contractile proteins from mouse liver nuclei J Cell Biol 70:440–452

    Google Scholar 

  • Damjanov I, Solter D, Belicza M, Skreb N (1971) Teratomas obtained through extrauterine growth of seven-day mouse embryos. J Nat Cancer Inst 46:471–480

    PubMed  Google Scholar 

  • Failly-Crépin C, Martin GR (1979) Protein synthesis and differentiation in a clonal line of teratocarcinoma and in preimplantation mouse embryo (1979) Cell Differ 8:61–73

    PubMed  Google Scholar 

  • Fletcher L, Rider CC, Taylor CB, Adamson ED, Luke BM, Graham CF (1978) Enolase isoenzymes as markers of differentiation in teratocarcinoma cells and normal tissues of mouse. Dev Biol 65:462–475

    PubMed  Google Scholar 

  • Franke WW (1971) Relationship of nuclear membrans with filaments and microtubules. Protoplasma 73:263–297

    PubMed  Google Scholar 

  • Franke WW, Schmid E, Osborn M, Weber K (1978) Different intermediatesized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci USA 75:5034–5038

    PubMed  Google Scholar 

  • Gardner RL (1978) The relationship between cell lineage and differentiation in the early mouse embryo. In: Gehring W (ed) Results and problems in cell differentiation, vol IX, Springer, Berlin Heidelberg New York, pp 205–241

    Google Scholar 

  • Gazit B, Panet A, Cedar H (1980) Reconstitution of a deoxyribonuclease I sensitive structure on active genes. Proc Natl Acad Sci USA 77:1787–1790

    PubMed  Google Scholar 

  • Graham CF (1977) Teratocarcinoma cells and normal mouse embryogenesis. In: Sherman MI (ed) Concepts in mammalian embryogenesis, MIT Press, Cambridge, pp 313–394

    Google Scholar 

  • Hnilica LS, Chiu JF, Hardy K, Fujitani H (1978) Chromosomal proteins in differentiation. In: Saunders GF (ed) Cell differentation and neoplasia, Kaven Press, New York, pp 325–346

    Google Scholar 

  • Illmensee K (1978) Reversion of malignancy and normalized differentiation of teratocarcinoma cells in chimeric mice. In: Russell LB (ed) Genetic mosaics and chimeras in mammals, Plenum New York pp 3–25

    Google Scholar 

  • Jacob F (1977) Mouse teratocarcinomas and embryonic antigens. Immunol Rev 33:3–22

    PubMed  Google Scholar 

  • Kemler R, Morello D, Jacob F (1979) Properties of some monoclonal antibodies raised against mouse embryonal carcinoma cells. In: Le Douarin N (ed). Cell lineage, stem cell and cell differentiation. INSERM symp. No 10, Elsevier/North-Holland Biomedical Press, pp 101–113

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    PubMed  Google Scholar 

  • LeStourgeon WM (1978) The occurrence of contractile proteins in nuclei and their possible function. In: Bush H (ed) The cell nucleus, Vol VI, Acad Press, New York, pp 305–326

    Google Scholar 

  • Loeb JE, Ritz E, Creuzet C, Jami J (1976) Comparison of chromosomal proteins of mouse primitive teratocarcinoma, liver and L cells. Exp Cell Res 103:450–454

    PubMed  Google Scholar 

  • MacGillivray AJ, Rickwood D (1974) The role of chromosomal proteins as gene regulators. In: Paul J (ed) Biochemistry of Cell Differentiation, Butterworths, London, pp 301–361

    Google Scholar 

  • Mayfield JE, Serunian LA, Silver LM, Elgin SCR (1978) A protein released by DNAase I digestion ofDrosophila nuclei is preferentially associated with puffs. Cell 14:539–544

    PubMed  Google Scholar 

  • Mintz B, Illmensee K (1975) Normal genetically mosaic mice produced from malignant teratocarcinoma cells. Proc Natl Acad Sci USA 72:3585–3589

    PubMed  Google Scholar 

  • Oakley BR, Kirsch DR, Morris NR (1980) A simplified ultrasensitive silver stain for detecting protein in polyacrylamide gels. Anal Biochem 105:361–363

    PubMed  Google Scholar 

  • O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    PubMed  Google Scholar 

  • Papaioannou VE, McBurney MW, Gardner RL, Evans MJ (1975) Fate of teratocarcinoma cells injected into early mouse embryos. Nature 258:70–73

    PubMed  Google Scholar 

  • Paulin D, Nicolas JF, Jacquet M, Jakob H, Gros F, Jacob F (1976) Comparative protein patterns in chromatins from mouse teratocarcinoma cells. Exp Cell Res 102:169–178

    PubMed  Google Scholar 

  • Peters KE, Commings DE (1980) Two-dimensional gel electrophoresis of rat liver nuclear washes, nuclear matrix, and hnRNA proteins. J Cell Biol 86:135–155

    PubMed  Google Scholar 

  • Pierce GB, Stevens LC, Nakane PK (1967) Ultrastructural analysis of the early development of teratocarcinomas. J Nat Cancer Inst 39:755–773

    Google Scholar 

  • Schaffner W, Weismann C (1973) A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal Biochem 56:502–514

    PubMed  Google Scholar 

  • Sherman MI, Solter D (eds) (1975) Teratomas and differentiation. Acad Press, New York

    Google Scholar 

  • Silver LM, Elgin SCR (1977) Distribution patterns of three subfractions ofDrosophila nonhistone chromosomal proteins: possible correlation with gene activity. Cell 11:971–983

    PubMed  Google Scholar 

  • Solter D, Knowles BB (1978) Monoclonal antibody defining stagespecific mouse embryonic antigen (SSEA-1). Proc Natl Acad Sci USA 75:5565–5569

    PubMed  Google Scholar 

  • Stevens LC (1970) The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev Biol 21:364–382

    PubMed  Google Scholar 

  • Weisbrod S, Groudine M, Weintraub H (1980) Interaction of HMG 14 and 17 with actively transcribed genes. Cell 19:289–301

    PubMed  Google Scholar 

  • Woodcock CLF (1980) Nucleus-associated intermediate filaments from chicken erythrocytes. J Cell Biol 85:881–889

    PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Animal Biology, University of Geneva, CH-1224, Geneva, Switzerland

    Horst Blüthmann & Karl Illmensee

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  1. Horst Blüthmann
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  2. Karl Illmensee
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Blüthmann, H., Illmensee, K. Nuclear nonhistone proteins in mouse teratocarcinomas. Wilhelm Roux' Archiv 190, 374–378 (1981). https://doi.org/10.1007/BF00863277

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  • DOI: https://doi.org/10.1007/BF00863277

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