Summary
The blastomeres of the zebra fish embryo can be classified into two types-cells stained densely (D) or lightly (L) with a mixture of toluidine and methylene (T-M) blue. The dense staining of D cells is largely due to the high density of mitochondria, rough endoplasmic reticulum and polyribosomes. The presence of partially dense stained cells during early blastula stage shows that L cells are transformed into D cells. That the yolk syncytial layer (YSL) plays some role in this transformation is suggested by the proximity of these cells to the YSL and by their distinct spatial orientation with densely stained cytoplasmic regions always facing towards the interior of the embryo.
References
Bodick N, Levinthal C (1980) Growing optic nerve fibers follow neighbours during embryogenesis. Proc Natl Acad Sci USA 77:4374–4378
Hisaoka KK, Battle HI (1958) The normal developmental stages of the zebra fish,Brachydanio rerio (Hamilton-Buchanan). J Morphol 102:311–327
Lentz TL, Trinkaus JP (1967) A fine structural study of cytodifferentiation during cleavage, blastula, and, gastrula stages ofFundulus hereroclitus. J Cell Biol 32:121–138
Monroy A, Maggio R, Rinaldi AM (1965) Experimentally induced activation of the ribosomes of the unfertilized sea urchin egg. Proc Natl Acad Sci USA 54:107–111
Reynolds ES (1963) The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J Cell Biol 17:208–212
Streisinger G, Walker C, Dower N, Knauber D, Singer F (1981) Production of clones of homozygous diploid zebra fish (Brachydanio rerio). Nature 291:293–296
Trinkaus JP (1969) Cells into Organs: The forces that shape the embryo. Prentice-Hall, Englewood Cliffe, New Jersey
Trinkaus JP (1976) On the mechanism of metazoan cell movements. In: Poste G, Nicolson GL (eds). The cell surface in animal embryogenesis and development. North-Holland, Amsterdam, pp 225–329
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Dev Dasgupta, J., Singh, U.N. Early differentiation in zebra fish blastula: Role of yolk syncytial layer. Wilhelm Roux' Archiv 190, 358–360 (1981). https://doi.org/10.1007/BF00863273
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DOI: https://doi.org/10.1007/BF00863273