A micromethod for the determination of carotene and vitamin A in whole blood

  • V. A. Karpacheva
Morphology and Pathomorphology


A microspectrophotometric method is proposed for determination of vitamin A and carotene in 0.1 ml of whole blood. The principle of the method is to obtain petrol-ether extracts from the nonsaponifying blood fraction, free from impurities which absorb within the 300–400 mm range. Direct spectrophotometry of the vitamin A and carotene in the extract obtained is then possible. The loss of vitamin A does not exceed 5–8%. The difference between parallel tests did not exceed the error of the instrument.


Public Health Carotene Parallel Test Blood Fraction Direct Spectrophotometry 
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Copyright information

© Consultants Bureau Enterprises, Inc. 1965

Authors and Affiliations

  • V. A. Karpacheva
    • 1
  1. 1.Laboratory of Biochemistry Institute of Experimental Pathology and TherapyAMN SSSRSukhumi

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