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Transforming growth factor α and epidermal growth factor expression in experimental murine polycystic kidney disease

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Abstract

Cystic change in polycystic kidney disease (PKD) is associated with epithelial hyperplasia, altered fluid and electrolyte transport, and de-differentiation of renal tubular epithelium. The role of polypeptide growth factors as potential modulators of cystic change remains an area of controversy. In this study, the expression of epidermal growth factor (EGF) and transforming growth factor-α (TGFα) were assessed by immunohistochemistry and image analysis in glucocorticoid-induced PKD in the newborn mouse. Newborn C3H mice received either 200 mg/kg methylprednisolone acetate (MPA) or 0.9% saline as a control. EGF expression was not detected in significant quantities in either MPA-treated or control animals. TGFα, however, was expressed in immature control kidney in a largely basolateral distribution. Expression increased significantly in association with cystic change in MPA-treated animals and was localized to the apical cell surface, implying altered polarity of secretion. There is no evidence that EGF is a mitogen in this early developmental model of PKD. TGFα, however, may be an important mediator of cystic change in immature or de-differentiated renal tubular epithelium.

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Authors and Affiliations

  1. Section of Nephrology, Department of Pediatrics and Child Health, University of Manitoba, AE102-840 Sherbrook Street, R3A 1S1, Winnipeg, Manitoba, Canada

    Malcolm R. Ogborn & Sanjay Sareen

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  1. Malcolm R. Ogborn
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  2. Sanjay Sareen
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Ogborn, M.R., Sareen, S. Transforming growth factor α and epidermal growth factor expression in experimental murine polycystic kidney disease. Pediatr Nephrol 10, 181–184 (1996). https://doi.org/10.1007/BF00862070

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  • DOI: https://doi.org/10.1007/BF00862070

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