Pediatric Nephrology

, Volume 10, Issue 2, pp 181–184 | Cite as

Transforming growth factor α and epidermal growth factor expression in experimental murine polycystic kidney disease

  • Malcolm R. Ogborn
  • Sanjay Sareen
Original Article


Cystic change in polycystic kidney disease (PKD) is associated with epithelial hyperplasia, altered fluid and electrolyte transport, and de-differentiation of renal tubular epithelium. The role of polypeptide growth factors as potential modulators of cystic change remains an area of controversy. In this study, the expression of epidermal growth factor (EGF) and transforming growth factor-α (TGFα) were assessed by immunohistochemistry and image analysis in glucocorticoid-induced PKD in the newborn mouse. Newborn C3H mice received either 200 mg/kg methylprednisolone acetate (MPA) or 0.9% saline as a control. EGF expression was not detected in significant quantities in either MPA-treated or control animals. TGFα, however, was expressed in immature control kidney in a largely basolateral distribution. Expression increased significantly in association with cystic change in MPA-treated animals and was localized to the apical cell surface, implying altered polarity of secretion. There is no evidence that EGF is a mitogen in this early developmental model of PKD. TGFα, however, may be an important mediator of cystic change in immature or de-differentiated renal tubular epithelium.

Key words

Polycystic kidneys Mouse Epidermal growth factor Transforming growth factor-α Glucocorticoids 


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Copyright information

© IPNA 1996

Authors and Affiliations

  • Malcolm R. Ogborn
    • 1
  • Sanjay Sareen
    • 1
  1. 1.Section of Nephrology, Department of Pediatrics and Child HealthUniversity of ManitobaWinnipegCanada

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