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Effect of lipoid nephrosis cytokine on glomerular sulfated compounds and albuminuria

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Abstract

Supernatants from peripheral blood mononuclear cells cultures of 30 idiopathic, minimal lesion, nephrotic syndrome (IMLNS) patients in relapse and the same patients in remission were fractionated by gel filtration chromatography. Fractions eluting with carbonic anhydrase (29 kilodaltons) were infused for 5 days at the rate of 10 μl/h into the left renal artery of Wistar rats using an Alzet osmotic pump. On the last day of infusion, rats were injected with35sulfate (1.0 mCi/200 g) intraperitoneally and killed after 8 h. Glomeruli were isolated and glomerular basement membrane (GBM) obtained. There was a significant increase in35sulfate uptake by GBM of the infused kidney (302±92 cpm/mg dry glomerular weight, mean±SEM) compared with the uptake seen in the contralateral kidney (157±36,P<0.01) when the fraction from IMLNS patients in relapse was infused. No significant differences in35sulfate incorporation were seen between infused kidney (166±41) and contralateral kidney (172±64) when the same fraction from patients in remission was administered. A significant increase in albuminuria was seen on the last day of infusion (14.2±1.0 mg/24 h, mean±SEM) when supernatant factor from IMLNS patients in relapse was used. No significant differences in urinary albumin excretion prior to and after infusion were seen when the same fraction from IMLNS patients in remission was administered. The in vivo infusion of supernatant factor from IMLNS patients in relapse increased the35sulfate uptake by GBM and augmented albuminuria, suggesting that the factor may have pathogenic significance in the proteinuria of IMLNS.

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References

  1. Mallick NP (1977) The pathogenesis of minimal change nephropathy. Clin Nephrol 7: 87–95

    PubMed  Google Scholar 

  2. Wilkinson AH, Gillespie C, Hartley B, Gwyn Williams D (1989) Increase in proteinuria and reduction in number of anionic sites in the glomerular basement membrane in rabbits by infusion of human nephrotic plasma in vivo. Clin Sci 77: 43–48

    PubMed  Google Scholar 

  3. Zimmerman SW (1984) Increased urinary protein excretion in the rat produced by serum from a patient with recurrent focal glomerular sclerosis after renal transplantation. Clin Nephrol 22: 32–38

    PubMed  Google Scholar 

  4. Boulton Jones JM, Tulloch I, Dore B, McLay A (1983) Changes in the glomerular capillary wall induced by lymphocyte products and serum of nephrotic patients. Clin Nephrol 20: 72–77

    PubMed  Google Scholar 

  5. Yoshizawa N, Kusumi Y, Matsumoto K, Oshima S, Takeuchi A, Kawamura O, Kubota T, Kondo S, Niwa H (1989) Studies of a glomerular permeability factor in patients with minimal-change nephrotic syndrome. Nephron 51: 370–376

    PubMed  Google Scholar 

  6. Mayurama K, Tomizawa S, Shimabukuro N, Fukuda T, Johshita T, Kurcume T (1989) Effect of supernatants derived from T lymphocyte culture in minimal change nephrotic syndrome on rat kidney capillaries. Nephron 51: 73–76

    PubMed  Google Scholar 

  7. Koyama A, Fujisaki M, Kobayashi M, Igarashi M, Narita M (1991) A glomerular permeability factor produced by human T cell hybridomas. Kidney Int 40: 453–460

    PubMed  Google Scholar 

  8. Tanaka R, Yoshikawa N, Nakamura H, Ito H (1992) Infusion of peripheral blood mononuclear cell products from nephrotic children increases albuminuria in rats. Nephron 60: 35–41

    PubMed  Google Scholar 

  9. Garin EH, Corontzes N (1992) Effect of lymphokine from nephrotic peripheral blood mononuclear cells on catabolism of rat glomerular basement membrane sulfated compounds. Nephron 62: 416–421

    PubMed  Google Scholar 

  10. Churg J, Habib R, White RHR (1970) Pathology of the nephrotic syndrome in children: a report for the International Study of Kidney Disease in Children. Lancet I: 1299–1302

    Google Scholar 

  11. Garin EH, Boggs KP (1985) Effect of peripheral blood mononuclear cells rom patients with nephrotic syndrome on uptake of sulfate-35 by glomerular basement membrane. Int J Pediatr Nephrol 6: 189–194

    PubMed  Google Scholar 

  12. Fong JCS, Drummond KN (1968) Methods for preparation of glomeruli for metabolic studies. J Lab Clin Med 71: 1034–1040

    PubMed  Google Scholar 

  13. Meezan E, Hjelle JT, Brendel K, Carlson EC (1975) A single versatile nondisruptive method for the isolation of morphologically and chemically pure basement membrane from several tissues. Life Sci 17: 1721–1732

    PubMed  Google Scholar 

  14. Beavan LA, Davies M, Mason RM (1988) Renal glomerular proteoglycans. An investigation of their synthesis in vivo using a technique for fixation in situ. Biochem J 251: 411–418

    PubMed  Google Scholar 

  15. First MR, Sloan DE, Pesce AJ, Pollack VE (1977) Albumin excretion by the kidney: the effects of volume expansion. J Lab Clin Med 89: 25–29

    PubMed  Google Scholar 

  16. Kanwar YS (1984) Biology of disease. Biophysiology of glomerular filtration and proteinuria. Lab Invest 51: 7–21

    PubMed  Google Scholar 

  17. Brenner BM, Bohrer MP, Baylis C, Deen WM (1977) Determinants of glomerular permselectivity: insights derived from observations in vivo. Kidney Int 12: 229–237

    PubMed  Google Scholar 

  18. Eisenbach GM, Van Liew JB, Boylan JW (1975) Effect of angiotensin on the filtration of protein in the rat kidney: a micropuncture study. Kidney Int 8: 80–87

    PubMed  Google Scholar 

  19. Ichikawa I, Brenner BM (1977) Evidence of glomerular actions of ADH and dibutyryl cyclic AMP in the rat. Am J Physiol 233: F102-F117

    PubMed  Google Scholar 

  20. Landwehr DM, Carvalho JS, Oken DE (1977) Micropuncture studies of the filtration and absorption of albumin by nephrotic rats. Kidney Int 11: 9–17

    PubMed  Google Scholar 

  21. Caulfield JP, Farquhar MG (1974) The permeability of glomerular capillaries to graded dextrans: identification of the basement membrane as the primary filtration barrier. J Cell Biol 63: 883–903

    PubMed  Google Scholar 

  22. Rennke HG, Cotran RS, Venkatachalam MA (1975) Role of molecular charge in glomerular permeability: tracer studies with cationized ferritins. J Cell Biol 67: 638–646

    PubMed  Google Scholar 

  23. Kanwar YS, Farquhar MG (1979) Anionic sites in the glomerular basement membrane: in vivo and in vitro localization to the laminae rarae by cationic probes. J Cell Biol 81: 137–153

    PubMed  Google Scholar 

  24. Kanwar YS, Farquhar MG (1979) Presence of heparan sulfate in the glomerular basement membrane. Proc Natl Acad Sci USA 76: 1300–1307

    Google Scholar 

  25. Rosenzweig IJ, Kanwar YS (1982) Removal of sulfated (heparan sulfate) or nonsulfated (hyaluronic acid) glycosaminoglycans results in increased permeability of the glomerular basement membrane to125I-bovine serum albumin. Lab Invest 47: 177–184

    PubMed  Google Scholar 

  26. Kanwar YS, Linker A, Farquhar MG (1980) Increased permeability of the glomerular basement membrane to ferritin after removal of glycosaminoglycans (heparan sulfate) by enzyme digestion. J Cell Biol 86: 688–693

    PubMed  Google Scholar 

  27. Guasch A, Hashimoto H, Sibley RK (1991) Glomerular dysfunction in nephrotic humans with minimal changes or focal glomerulosclerosis. Am J Physiol 260: F728-F737

    PubMed  Google Scholar 

  28. Robson AM, Giangiacomo J, Kienstra RA, Naqvi ST, Ingelfinger JR (1974) Normal glomerular permeability and its modification by minimal change nephrotic syndrome. J Clin Invest 54: 1190–1199

    PubMed  Google Scholar 

  29. Mahan JD, Sisson SP, Vernier RL (1985) Altered glomerular basement membrane (GBM) anionic sites in the minimal change nephrotic syndrome (MCMNS) in man (abstract). Kidney Int 27: 217

    Google Scholar 

  30. Washizawa K, Kasai S, Mori T, Komiyama A, Shigematsu H (1993) Ultrastructural alteration of glomerular anionic sites in nephrotic patients. Pediatr Nephrol 7: 1–5

    PubMed  Google Scholar 

  31. Born J van der, Heuval PWJ van der, Bakker MAH, Veerkamp JH, Assmann KJM, Weening JJ, Berden JHM (1993) Distribution of GBM heparan sulfate proteoglycan core protein and side changes in human glomerular diseases. Kidney Int 43: 454–463

    PubMed  Google Scholar 

  32. Gang NF, Mautner W (1972) Studies on the mechanism of the onset of proteinuria in aminonucleoside nephrosis. Lab Invest 27: 310–316

    PubMed  Google Scholar 

  33. Salant DJ, Belok S, Madaio MP, Couser WG (1980) A new role for complement in experimental membranous nephropathy in rats. J Clin Invest 66: 1339–1350

    PubMed  Google Scholar 

  34. Wada T, Tomosugi N, Naito T, Yokoyama H, Kobayashi K, Harada A, Mukaida N, Matsushima K (1994) Prevention of proteinuria by the administration of anti-interleukin 8 antibody in experimental acute immune complex-induced glomerulonephritis. J Exp Med 180: 1135–1140

    PubMed  Google Scholar 

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  1. Division of Nephrology, Department of Pediatrics, University of South Florida, 17 Davis Boulevard, 2nd Floor, 33606, Tampa, FL, USA

    Eduardo H. Garin

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  1. Eduardo H. Garin
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Garin, E.H. Effect of lipoid nephrosis cytokine on glomerular sulfated compounds and albuminuria. Pediatr Nephrol 9, 587–593 (1995). https://doi.org/10.1007/BF00860943

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  • DOI: https://doi.org/10.1007/BF00860943

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