Skip to main content
SpringerLink
Log in

Studies of terminal differentiation of electrolyte transport in the renal proximal tubule using short-term primary cultures

  • Physiology Review
  • Published:
Pediatric Nephrology Aims and scope Submit manuscript

Abstract

There are several lines of indirect evidence suggesting that the renal tubule cells have not yet reached terminal differentiation at birth. Methods used in cell biology can now be applied to study renal ontogeny. This review describes how primary cultures of proximal tubule cells from rats can be used to investigate developmental changes in Na permeability and Na-K-ATPase-mediated transport.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aperia A (1983) Salt and water metabolism in the perinatal period. In: Battaglia FC, Boyd RDH (eds) Pediatrics, vol 2. Perinatal medicine. Butterworth, London, pp 113–130

    Google Scholar 

  2. Aperia A, Herin P, Zetterström R (1985) Sodium, chloride and potassium needs in very low birthweight infants. In: Tsang RC (eds) Vitamin and mineral requirements in preterm infants. Dekker, New York, pp 137–151

    Google Scholar 

  3. Larsson L, Horster M (1976) Ultrastructure and net fluid transport in isolated perfused developing proximal tubules. J Ultrastruct Res 54: 276–285

    PubMed  Google Scholar 

  4. Schmidt U, Horster M (1977) Na-K-activated ATPase: activity maturation in rabbit nephron segments dissected in vitro. Am J Physiol 233: F55-F60

    PubMed  Google Scholar 

  5. Horster M (1978) Loop of Henle functional differentiation in vitro perfusion of the isolated thick ascending segment. Pflugers Arch 378: 15–24

    PubMed  Google Scholar 

  6. Aperia A, Larsson L (1979) Correlation between fluid reabsorption and proximal tubule ultrastructure during development of the rat kidney. Acta Physiol Scand 105: 11–22

    PubMed  Google Scholar 

  7. Aperia A, Larsson L, Zetterström R (1981) Hormonal induction of NaKATPase in developing proximal tubular cells. Am J Physiol 241: C356-C360

    Google Scholar 

  8. Schwartz GJ, Evan AP (1983) Development of solute transport in rabbit proximal tubule. I. HCO3 − and glucose absorption. Am J Physiol 245: F382-F390

    PubMed  Google Scholar 

  9. Evan AP, Gattone II VH, Schwartz GJ (1983) Development of solute transport in rabbit proximal tubule. II. Morphologic segmentation. Am J Physiol 245: F391-F407

    PubMed  Google Scholar 

  10. Schwartz GJ, Evan AP (1984) Development of solute transport in rabbit proximal tubule. III. Na-K-ATPase activity. Am J Physiol 246: F845-F852

    PubMed  Google Scholar 

  11. Rane S, Aperia A (1985) Ontogeny of NaKATPase activity in thick ascending limb and concentrating capacity. Am J Physiol 249: F723-F728

    PubMed  Google Scholar 

  12. Handler JS, Perkins FM, Johnson JP (1980) Studies of renal cell function using cell culture techniques. Am J Physiol 238: F1-F9

    PubMed  Google Scholar 

  13. Lever JE (1986) Expression of differentiated functions in kidney epithelial cell lines. Min Electrolyte Metab 12: 14–19

    Google Scholar 

  14. Salas PJ, Misek DE, Vega-Salas DE, Gundersen D, Cereijido M, Rodriguez-Boulan E (1986) Microtubules and actin filaments are not critically involved in the biogenesis of epithelial cell surface polarity. J Cell Biol 102: 1853–1867

    PubMed  Google Scholar 

  15. kartha S, Sukhatme VP, Toback FG (1987) ADP activates protooncogene expression in renal epithelial cells. Am J Physiol 252: F1175-F1179

    PubMed  Google Scholar 

  16. Preston AS, Muller J, Handler JS (1988) Dexamethasone accelerates differentiation of A6 epithelia and increases response to vasopressin. Am J Physiol 255: C661-C666

    PubMed  Google Scholar 

  17. Taub M, Sato G (1980) Growth of functional primary cultures of kidney epithelial cells in defined medium. J Cell Physiol 105: 369–378

    PubMed  Google Scholar 

  18. Walsh-Reitz MM, Aithal HN, Toback FG (1984) Na regulates growth of kidney epithelial cells induced by lowering extracellular K concentration. Am J Physiol 247: C321-C326

    PubMed  Google Scholar 

  19. Lechene C (1986) Electron probe analysis of cultured cells. In: Somylo AP (ed) Recent advances in electron and light optical imaging in biology and medicine. N. Y. Academy of Sciences, New York, pp 270–283

    Google Scholar 

  20. Rozengurt E (1986) Early signals in the mitogenic response. Science 234: 161–166

    PubMed  Google Scholar 

  21. Chambard JC, Pouyssegur J (1986) Intracellular pH controls growth factor induced ribosomal protein S6 phosphorylation and protein synthesis in the G0-G1 transition of fibroblasts. Exp Cell Res 164: 282–294

    PubMed  Google Scholar 

  22. Valk J van der, Verlaan I, Laat SW de, Moolenaar WH (1987) Expression of pp60v-src alters the ionic permeability of the plasma membrane in rat cells. J Biol Chem 262: 2431–2434

    PubMed  Google Scholar 

  23. Dulbecco R (1970) Topoinhibition and serum requirement of transformed and untransformed cells. Nature 227: 802–806

    PubMed  Google Scholar 

  24. Chung SD, Alavi N, Livingston D, Hiller S, Taub M (1982) Characterization of primary rabbit kidney cultures that express proximal tubule functions in a hormonally defined medium. J Cell Biol 95: 118–126

    PubMed  Google Scholar 

  25. Horster M (1979) Primary culture of mammalian nephron epithelia: requirements for cell outgrowth and proliferation from defined explanted nephron segments. Pflugers Arch 382: 209–215

    PubMed  Google Scholar 

  26. Stanton RC, Mendrick DL, Rennke HG, Seifter JL (1986) Use of monoclonal antibodies to culture rat proximal tubule cells. Am J Physiol 251: C780-C786

    PubMed  Google Scholar 

  27. Gilbert SF, Migcon BR (1977) Renal enzymes in kidney cells selected by D-valine medium. J Cell Physiol 92: 161–168

    PubMed  Google Scholar 

  28. Fine LG, Sakhrani LM (1986) Proximal tubular cells in primary culture. Min Electrolyte Metab 12: 51–57

    Google Scholar 

  29. Harris RC, Seifter JL, Lechene C (1986) Coupling of Na−H exchange and Na−K pump activity in cultured rat proximal tubule cells. Am J Physiol 251: C815-C824

    PubMed  Google Scholar 

  30. Fine LG, Badie-Dezfooly B, Lowe AG, Hamzeh A, Wells J, Salehmoghaddam S (1985) Stimulation of Na+/H+ antiport is an early event in hypertrophy of renal proximal tubular cells. Proc Natl Acad Sci USA 82: 1736–1740

    PubMed  Google Scholar 

  31. Fine LG, Holley RW, Nasri H, Badie-Dezfooly B (1985) BSC-1 growth inhibitor transforms a mitogenic stimulus into a hypertrophic stimulus for renal proximal tubular cells: relationship to Na+/H+ antiport activity. Proc Natl Acad Sci USA 82: 6163–6166

    PubMed  Google Scholar 

  32. Stanton RC, Seifter JL (1988) Epidermal growth factor rapidly activates the hexose monophosphate shunt in kidney cells. Am J Physiol 253: C267-C271

    Google Scholar 

  33. Sakhrani LM, Badie-Dezfooly B, Trizna W, Mikhail N, Lowe AG, Taub M, Fine LG (1984) Transport and metabolism of glucose by renal proximal tubular cells in primary culture. Am J Physiol 246: F757-F764

    PubMed  Google Scholar 

  34. Norman J, Badie-Dezfooly B, Nord EP, Kurtz I, Schlosser J, Chaudhari A, Fine LG (1987) EGF-induced mitogenesis in proximal tubular cells: potentiation by angiotensin II. Am J Physiol 253: F299-F309

    PubMed  Google Scholar 

  35. Becker JH, Willis JS (1979) Properties of Na−K pump in primary cultures of kidney cells. J Cell Physiol 99: 427–440

    PubMed  Google Scholar 

  36. Larsson S, Aperia A, Lechene C (1986) Studies on final differentiation of rat renal proximal tubular cells in culture. Am J Physiol 251: C455-C464

    PubMed  Google Scholar 

  37. Larsson SH, Aperia A, Lechene C (1988) Studies on terminal differentiation of rat renal proximal tubular cells in culture: ouabain-sensitive Na and K transport. Acta Physiol Scand 132: 129–134

    PubMed  Google Scholar 

  38. Larsson L, Aperia A, Lechene C (1986) Ionic transport in individual renal epithelial cells from adult and young rats. Acta Physiol Scand 126: 321–332

    PubMed  Google Scholar 

  39. Abraham EH, Breslow JL, Epstein J, Chang-Sing P, Lechene C (1985) Preparation of individual human diploid fibroblasts and study of ion transport. Am J Physiol 248: C154-C164

    PubMed  Google Scholar 

  40. Aperia A, Haldosén L-A, Larsson L, Gustafsson J-A (1985) Ontogeny of triamcinolone-acetonide binding sites in outer cortical tissue from rat kidneys. Am J Physiol 249: F891-F897

    PubMed  Google Scholar 

  41. Lechene C, Harris RC (1987) Electron probe analysis of cultured renal cells. In: Brenner BM, Stein JH (eds) Contemporary issues in nephrology: modern techniques of ion transport. Churchill Livingstone, New York, pp 173–197

    Google Scholar 

  42. Larsson L, Aperia A, Wilton P (1980) Effect of normal development on compensatory renal growth. Kidney Int 18: 29–35

    PubMed  Google Scholar 

  43. Larsson L (1977) The ultrastructure of the developing renal proximal tubule cells as related to functional maturation. Academic thesis, Aarhus University, Denmark

    Google Scholar 

  44. Larsson SH, Kölare S, Hultgård-Nilsson, Aperia A (1989) The response of proximal tubular cells (PTC) to growth factors changes during terminal differentiation. Kidney Int 35: 314

    Google Scholar 

  45. Larsson SH, Kölare S, Aperia A (1989) The proliferative response of proximal tubular cells from weanling (W) and adolescent (A) rats to growth factors. International Pediatric Nephrology Association, September 1989

Download references

Author information

Authors and Affiliations

  1. Department of Paediatrics, St. Göran's Children's Hospital, S-11281, Stockholm, Sweden

    S. H. Larsson, L. Larsson & A. Aperia

  2. NEPRAC and Laboratory of Cellular Physiology, Harvard Medical School and Brigham and Women's Hospital, 02115, Boston, MA, USA

    C. Lechene

Authors
  1. S. H. Larsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Larsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Lechene
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Aperia
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Larsson, S.H., Larsson, L., Lechene, C. et al. Studies of terminal differentiation of electrolyte transport in the renal proximal tubule using short-term primary cultures. Pediatr Nephrol 3, 363–368 (1989). https://doi.org/10.1007/BF00858547

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00858547

Key words

Search

Navigation