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Microheterogeneity of urinary albumin and tubular proteinuria in juvenile diabetes mellitus

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Abstract

We studied differential urinary albumin excretion by a double one-dimensional gel electrophoresis with decyl sodium sulphate-polyacrylamide gel electrophoresis in the first, and isoelectric focusing in the second dimension in 37 diabetic children and 20 healthy subjects. In addition, total proteins, albumin, β2-microglobulin and molecular size distribution of urinary proteins were measured. the latter using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Whilst albuminuria was not significantly different from controls we found an increased microheterogeneity of urinary albumin in 38% of patients. In addition, low molecular weight protein (P<0.05) and β2-microglobulin excretion (P<0.01) were elevated. It is suggested that the appearance of highly heterogenous albumin in the pI range of 5.3–5.9 is the result of a decreased tubular reabsorption.

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Authors and Affiliations

  1. Division of Pediatric Nephrology and Metabolic Disorders, University Children's Hospital, Im Neuenheimer Feld 150, W-6900, Heidelberg, Federal Republic of Germany

    Martin Ries, Karl Schärer, Reinhard Wartha & Hildgund Schmidt

  2. Division of Pediatric Nephrology and Metabolic Disorders, University Children's Hospital, Würzburg, Federal Republic of Germany

    Dieter Gekle

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  1. Martin Ries
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  2. Karl Schärer
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  3. Reinhard Wartha
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  4. Hildgund Schmidt
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  5. Dieter Gekle
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Ries, M., Schärer, K., Wartha, R. et al. Microheterogeneity of urinary albumin and tubular proteinuria in juvenile diabetes mellitus. Pediatr Nephrol 5, 582–586 (1991). https://doi.org/10.1007/BF00856644

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  • DOI: https://doi.org/10.1007/BF00856644

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