Pediatric Nephrology

, Volume 8, Issue 4, pp 447–450 | Cite as

Transplacental effects of gentamicin on endocytosis in rat renal proximal tubule cells

  • Hatem Smaoui
  • Madeleine Schaeverbeke
  • Jean-Pierre Mallié
  • Jean Schaeverbeke
Original Article


Changes in kidney maturation in utero have been reported after gentamicin administration to pregnant rats. While the proteinuria commonly observed, could be related to modifications of the glomerular basement membrane, perturbed renal protein handling could be accounted for by changes in the proximal tubular cells. Therefore, we studied the effect of gentamicin on the renal handling and transport of proteins in proximal tubular cells using the horseradish peroxidase, a fluid-phase marker, as a probe. Gentamicin was administered intraperitoneally to pregnant Wistar rats (75 mg/kg body weight per day) and neonatal kidneys were studied 1 day after birth. In proximal tubular cells of the deep cortical area, containing the fully matured nephrons of neonates, the transport and digestion of reabsorbed peroxidase was considerably reduced compared with controls where peroxidase reached lysosomes after endocytosis. Urinary protein excretion increased in treated animals. We conclude that gentamicin, entering the proximal tubular cells via the endocytic pathway, decreases the tubular reabsorption of proteins, thus increasing urinary protein excretion.

Key words

Gentamicin Proximal tubular cells Endocytosis Gestation Neonate 


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Copyright information

© IPNA 1994

Authors and Affiliations

  • Hatem Smaoui
    • 1
  • Madeleine Schaeverbeke
    • 1
  • Jean-Pierre Mallié
    • 2
  • Jean Schaeverbeke
    • 1
  1. 1.Laboratoire de Biologie CellulaireUniversité Paris 7Paris Cedex 05France
  2. 2.Laboratoire de NéphrologieUniversité de Nancy IVandoeuvre CedexFrance

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