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Intraperitoneal production of erythropoietin with continuous ambulatory peritoneal dialysis

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Abstract

Higher hematocrit and serum erythropoietin (EPO) levels have previously been shown in end-stage renal disease patients treated with continuous ambulatory peritoneal dialysis (CAPD) compared with hemodialysis. We investigated whether EPO was produced intraperitoneally in CAPD patients. EPO concentration was 3.5±0.3 mU/ml by radioimmunoassay in 26 samples of peritoneal dialysis effluent obtained from 15 CAPD patients. EPO was not detectable in the fresh unused dialysate. No correlation was observed between EPO levels in the serum and dialysis effluent. Peritoneal macrophages were isolated from the dialysis effluent of 9 CAPD patients after an overnight dwell. The culture supernatant obtained after 24 h of in vitro culture of a million cells yielded EPO of 3.5±0.3 mU/ml. Our study demonstrated that peritoneal macrophages from CAPD patients produce EPO on in vitro stimulation, and EPO is present in the dialysis effluent of CAPD patients.

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Authors and Affiliations

  1. Department of Pediatrics, North Shore University Hospital-Cornell University Medical College, 11030, Manhasset, NY, USA

    Manju Chandra, Indira Sahdev & Melinda McVicar

  2. Department of Medicine, North Shore University Hospital-Cornell University Medical College, 11030, Manhasset, NY, USA

    Peter Bluestone

  3. Division of Biology and Medicine, Lawrence Berkeley Laboratories, University of California, Berkeley, California, USA

    Gisela Clemons

Authors
  1. Manju Chandra
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  2. Gisela Clemons
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  3. Indira Sahdev
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  4. Melinda McVicar
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  5. Peter Bluestone
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Chandra, M., Clemons, G., Sahdev, I. et al. Intraperitoneal production of erythropoietin with continuous ambulatory peritoneal dialysis. Pediatr Nephrol 7, 281–283 (1993). https://doi.org/10.1007/BF00853222

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  • DOI: https://doi.org/10.1007/BF00853222

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