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Pediatric Nephrology

, Volume 3, Issue 4, pp 386–390 | Cite as

P-fimbriae vaccines

I. Cross reactive antibodies to heterologous P-fimbriae
  • M. Bernice Kaack
  • Auli Pere
  • Timo K. Korhonen
  • Stefan B. Svenson
  • James A. Roberts
Original Article

Abstract

To test for cross-protective capacity of two different P-fimbriae vaccines we vaccinated baboons with fimbriae purified from eitherEscherichia coli strain ER2 or strain JR1. The vaccinated animals showed elevated antibody titers to P-fimbriae from each of theE. coli strains used, suggesting cross-reactivity as was expected from the results of immunoprecipitation of the fimbriae. Enzyme-linked immunosorbent assay inhibition by heterologous P-fimbriae proved this to be true immunologic cross-reactivity.

Key words

P-fimbriae Vaccines Antibodies, cross-reactive Escherichia coli 

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References

  1. 1.
    Achtman MA, Mercer B, Kusecek A, Pohl M, Heuzenroeder W, Aaronson W, Sutton A, Silver RP (1983) Six widespread bacterial clones amongEscherichia coli K1 isolates. Infect Immun 39: 315–335Google Scholar
  2. 2.
    Källenius G, Möllby R, Svenson SB, Winberg J, Lundblad A, Svenson S, Cedergren B (1980) The pk antigen as receptor for the haemagglutinin of pyelonephriticEscherichia coli. FEMS Microbiol Lett 7: 297–302Google Scholar
  3. 3.
    Källenius G, Möllby R, Svenson SB, Helin I, Hultberg H, Cedergren B, Winberg J (1981) Occurrence of P-fimbriatedEscherichia coli in urinary tract infections. Lancet II: 1369–1372Google Scholar
  4. 4.
    Korhonen T, Väisänen V, Saxen H, Hultberg H, Svenson SB (1982) P-antigen-recognizing fimbriae from human uropathogenicEscherichia coli strains. Infect Immun 37: 286–291Google Scholar
  5. 5.
    Leffler H, Svanborg-Edén C (1980) Chemical identification of a glycospingolipid receptor forEscherichia coli attaching to human urinary tract epithelial cells and agglutinating erythrocytes. FEMS Microbiol Lett 8: 127–134Google Scholar
  6. 6.
    Väisänen-Rhen V, Elo J, Väisänen E, Siitonen A, Ørskov I, Ørskov F, Svenson SB, Mäkelä PH, Korhonen TK (1984) P-fimbriated clones among uropathogenicEscherichia coli strains. Infect Immun 43: 149–155Google Scholar
  7. 7.
    Pere A, Nowicki B, Saxen H, Siitonen A, Korhonen TK (1987) Expression of P, Type-1 and Type-1C fimbriae ofEscherichia coli in the urine of patients with acute urinary tract infection. J Infect Dis 156: 567–574Google Scholar
  8. 8.
    Svenson SB, Källenius G, Möllby R, Hultberg H, Winberg J (1982) Rapid identification of P-fimbriatedE. coli by a receptor specific particle agglutination test. Infection 10: 209–214Google Scholar
  9. 9.
    Hagberg L, Engberg I, Freter R, Lam J, Olling S, Svanborg Edén C (1983) Ascending unobstructed urinary tract infection in mice caused by pyelonephritogenicEscherichia coli of human origin. Infect Immun 40: 273–283Google Scholar
  10. 10.
    O'Hanley P, Lark D, Falkow S, Schoolnik G (1985) Molecular basis ofEscherichia coli colonization of the upper urinary tract in BALB/c mice. J Clin Invest 75: 347–360Google Scholar
  11. 11.
    Lund B, Marklund BI, Stromberg N, Lindberg F, Karlsson KA, Normark S (1988) UropathogenicEscherichia coli can express serologically identical pili of different receptor binding specificities. Mol Microbiol 2: 255–263Google Scholar
  12. 12.
    Pere A, Nowicki B, Saxen H, Siitonen A, Korhonen TK (1987) Expression of P, type-1 and type-1C fimbriae ofEscherichia coli in the urine of patients with acute urinary tract infection. J Infect Dis 156: 567–574Google Scholar
  13. 13.
    Domingue GJ, Laucirica R, Baliga P, Covington S, Robledo JA, Li S (1988) Virulence of wild-typeE. coli uroisolates in experimental pyelonephritis. Kidney Int 34: 761–765Google Scholar
  14. 14.
    Roberts JA, Kaack B, Källenius G, Möllby R, Winberg J, Svenson SB (1984) Receptors for pyelonephritogenicEscherichia coli in primates. J Urol 131: 163–168Google Scholar
  15. 15.
    Svenson SB, Hultberg H, Källenius G, Korhonen TK, Möllby R, Winberg J (1983) P-fimbriae of pyelonephritogenicE. coli: identification and chemical characterization of receptors. Infection 11: 61–67Google Scholar
  16. 16.
    Roberts JA, Hardaway K, Kaack B, Fussell EN, Baskin G (1984) Prevention of pyelonephritis by immunization with P-fimbriae. J Urol 131: 602–607Google Scholar
  17. 17.
    Kaack MB, Roberts JA, Baskin G, Patterson GM (1988) Maternal immunization with P fimbriae for the prevention of neonatal pyelonephritis. Infect Immun 56: 1–6Google Scholar
  18. 18.
    Evans DG, Evans DJ Jr (1978) New surface-associated heat-labile colonization factor antigen (CFA-II) produced by enterotoxigenicEscherichia coli of serographs 06 and 08. Infect Immun 21: 638–647Google Scholar
  19. 19.
    Ofek I, Mirelman D, Sharon N (1977) Adherence ofEscherichia coli to human mucosal cells mediated by mannose receptors. Nature 265: 623–625Google Scholar
  20. 20.
    Janson KL, Roberts JA (1978) Experimental pyelonephritis. V. Functional characteristis of pyelonephritis. Invest Urol 15: 397–400Google Scholar
  21. 21.
    Roberts JA (1975) Experimental pyelonephritis in the monkey. III. Pathophysiology of ureteral malfunction induced by bacteria. Invest Urol 13: 117–120Google Scholar
  22. 22.
    Roberts JA, Roth JK Jr, Domingue G, Lewis RW, Kaack B, Baskin G (1982) Immunology of pyelonephritis in the primate model. V. Effect of superoxide dismutase. J Urol 128: 1394–1400Google Scholar
  23. 23.
    Roberts JA, Suarez GM, Kaack B, Källenius G, Svenson SB (1985) Experimental pyelonephritis in the monkey. VII. Ascending pyelonephritis in the absence of vesicoureteral reflux. J Urol 133: 1068–1075Google Scholar
  24. 24.
    Roberts JA, Kaack B, Morvant AB (1988) Vesicoureteral reflux in the primate. IV. Infection as cause of prolonged high-grade reflux. Pediatrics 82: 91–95Google Scholar
  25. 25.
    Rhen M, Wahlstrom E, Korhonen TK (1983) P-fimbriae ofEscherichia coli: fractionation by immune precipitation. FEMS Microbiol Lett 18: 227–232Google Scholar
  26. 26.
    Rhen M, Klemm P, Wahlstrom E, Svenson SB, Källenius G, Korhonen TK (1983) P-fimbriae ofEscherichia coli: immuno- and protein-chemical characterization of fimbriae from two pyelonephritogenic strains. FEMS Microbiol Lett 18: 233–238Google Scholar
  27. 27.
    Korhonen TK, Nurmiaho E, Ranta H, Svanborg-Edén C (1980) New method for isolation of immunologically pure pili fromEscherichia coli. Infect Immun 27: 569–575Google Scholar
  28. 28.
    Shachner MS, Miniter PM, Mayrer AR, Andriole VT (1987) Interaction of Tamm-Horsfall protein with bacterial extracts. Kidney Int 31: 77–84Google Scholar
  29. 29.
    Westphal O, Jann K (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. In: Whistler RL (ed) Methods in carbohydrate chemistry, vol 5. Academic Press, New York, p 83–91Google Scholar
  30. 30.
    Pere A, Leinonen M, Väisänen-Rhen V, Rhen M, Korhonen TK (1985) Occurrence of type-1C fimbriae onEscherichia coli strains isolated from human extraintestinal infections. J Gen Microbiol 131: 1705–1711Google Scholar
  31. 31.
    Orndorff PE (1987) Genetic study of piliation inEscherichia coli: Implications for understanding microbe-host interactions at the molecular level. Pathol Immunopathol Res 6: 82–92Google Scholar
  32. 32.
    Low D, Robinson EN Jr, McGee ZA, Falkow S (1987) The frequency of expression of pyelonephritis-associated pili is under regulatory control. Mol Microbiol 1: 335–346Google Scholar
  33. 33.
    Lund B, Lindberg F, Marklund B, Normark S (1988) Tip proteins of pili associated with pyelonephritis: new candidates for vaccine development. Vaccine 6: 110–112Google Scholar

Copyright information

© IPNA 1989

Authors and Affiliations

  • M. Bernice Kaack
    • 1
    • 2
    • 4
  • Auli Pere
    • 3
    • 4
  • Timo K. Korhonen
    • 3
    • 4
  • Stefan B. Svenson
    • 1
    • 4
  • James A. Roberts
    • 1
    • 2
    • 4
  1. 1.Department of UrologyDelta Regional Primate Research CenterCovingtonUSA
  2. 2.Department of UrologyTulane Medical SchoolNew OrleansUSA
  3. 3.Department of General MicrobiologyUniversity of HelsinkiHelsinkiFinland
  4. 4.Department of MicrobiologyThe National Bacteriological LaboratoryStockholmSweden

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