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Actin messenger in maternal RNP particles from an insect embryo (Smittia spec., Chironomidae, Diptera)

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Summary

Actin synthesis was observed in early embryos of the chironomid midgeSmittia spec. The criteria used to identify newly synthesized actin include comigration with purified actin in two-dimensional gel electrophoresis, and peptide mapping after limited proteolysis. Actin was also identified after in vitro translation of RNA or salt washed RNP-particles isolated from newly deposited eggs. While intact RNP-particles stimulated protein synthesis only poorly, the rate of synthesis increased considerably after partial or complete removal of proteins from RNP-particles. Actin was synthesized both during advanced stages of oogenesis and during early embryogenesis. In embryos during intravitelline cleavage actin synthesis was insensitive to inhibitors of transcription (actinomycin D, α-amanitin). Actin was found everywhere along the longitudinal axis of the embryo using a procedure which allowed simultaneous sectioning of a large number of embryos.

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References

  • Amsterdam A, Lindner HR, Gröschel-Stewart U (1977) Localization of actin and myosin in the rat oocyte and follicular wall by immunofluorescence. Anat Rev 187:311–328

    Google Scholar 

  • Bonner WM, Laskey RA (1974) A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem 46:83–88

    PubMed  Google Scholar 

  • Civelli O, Vincent A, Buri J-F, Scherrer K (1976) Evidence for a translational inhibitor linked to globin mRNA in untranslated free cytoplasmic messenger ribonucleoprotein complexes. FEBS Lett 72:71–76

    PubMed  Google Scholar 

  • Clark TG, Merriam RW (1977) Diffusible and bound actin in nuclei ofXenopus laevis oocytes. Cell 12:883–891

    PubMed  Google Scholar 

  • Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252:1102–1106

    PubMed  Google Scholar 

  • Davidson EH (1976) Gene activity in early development, 2nd edn. Academic Press, New York

    Google Scholar 

  • Franke WW, Rathke PC, Seib E, Trendelenburg MF, Osborn M, Weber K (1976) Distribution and mode of arrangement of microfilamentous structures and actin in the cortex of the amphibian oocyte. Cytobiologie 14:111–130

    PubMed  Google Scholar 

  • Garrels JI, Gibson W (1976) Identification and characterization of multiple forms of actin. Cell 9:793–805

    PubMed  Google Scholar 

  • Goldman R, Pollard T, Rosenbaum J (1976) Cell motility, A-C Cold Spring Harbor Laboratory, New York

    Google Scholar 

  • Green LH, Brandis JW, Turner FR, Raff RA (1975) Cytoplasmic microtubule proteins of the embryo ofDrosophila melanogaster. Biochem. 14:4487–4491

    Google Scholar 

  • Grosfeld H, Littauer UZ (1975) Cryptic form of mRNA in dormantArtemia salina cysts. Biochem Biophys Res Commun 67:176–181

    PubMed  Google Scholar 

  • Gross KW, Jacobs-Lorena M, Baglioni C, Gross PR (1973) Cell-free translation of maternal messenger RNA from sea urchin eggs. Proc Natl Acad Sci USA 70:2614–2618

    PubMed  Google Scholar 

  • Jäckle H (1979) Degradation of maternal poly(A)-containing RNA during early embryogenesis ofSmittia spec, (Chironomidae, Diptera) Wilhelm Roux's Archives 187:179–193

    Google Scholar 

  • Jäckle H, Kalthoff K (1979) RNA and protein synthesis in developing embryos ofSmittia spec. (Chironomidae, Diptera) Wilhelm Roux's Archives 187:283–305

    Google Scholar 

  • Jeffery WR (1977) Characterization of polypeptides associated with messenger RNA and its polyadenylate segment in Ehrlich Ascites messenger ribonucleoprotein. J Biol Chem 252:3525–3532

    PubMed  Google Scholar 

  • Jenkins NA, Kaumeyer JF, Young EM, Raff RA (1978) A test for masked message: the template activity of messenger ribonucleoprotein particles isolated from sea urchin eggs. Dev Biol 63:279–298

    PubMed  Google Scholar 

  • Kalthoff K, Sander K (1968) Der Entwicklungsgang der Mißbildung ‘Doppelabdomen’ im partiell UV-bestrahlten Ei vonSmittia parthenogenetica (Diptera, Chironomidae). Wilhelm Roux's Archives 161:129–146

    Google Scholar 

  • Laskey RA, Mills AD (1975) Quantitative film detection of3H and14C in polyacrylamide gels by fluorography. Eur J Biochem 56:335–341

    PubMed  Google Scholar 

  • Lovett JA, Goldstein ES (1977) The cytoplasmic distribution and characterization of poly(A)+RNA in oocytes and embryos ofDrosophila. Dev Biol 61:70–78

    PubMed  Google Scholar 

  • Mabuchi I (1973) A myosin-like protein in the cortical layer of the sea urchin egg. J Cell Biol 59:542–547

    PubMed  Google Scholar 

  • Mabuchi I (1974) A myosin-like protein in the cortical layer of cleaving starfish eggs. J Biochem 76:47–55

    PubMed  Google Scholar 

  • Moser JG, Bode HJ, Nüneman H, Collatz S, Feldhege A, Herzfeld A (1970) Differenzierung des Actinomyosinsystems während der Morphogenese der Hausgrille,Acheta domesticus I. Verh Dtsch Zool Ges 64:56–60

    Google Scholar 

  • Neeleman L, Vlogen-Donting L Van (1978) Excess of micrococcal nuclease may harm the exogenous mRNA in the mRNA-dependent rabbit reticulocyte cell-free system. FEBS Lett 95:103–106

    PubMed  Google Scholar 

  • Paglia LM, Kastern WH, Berry SJ (1976) Messenger ribonucleo-protein particles in silkmoth oogenesis. Dev Biol 51:182–189

    PubMed  Google Scholar 

  • Pagoulatos GM, Yaniv M (1978) Proteins bound to heterogenous nuclear RNA of Simian-Virus-40-infected cells. Eur J Biochem 91:1–10

    PubMed  Google Scholar 

  • Pelham HRB, Jackson RJ (1976) An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem 67:247–256

    PubMed  Google Scholar 

  • Raff RA, Colot HV, Selvig SE, Gross PR (1972) Oogenetic origin of messenger RNA for embryonic synthesis of microtubule proteins. Nature (London) 235:211–214

    Google Scholar 

  • Schmidt O, Jäckle H (1978) RNA synthesized during oogenesis and early embryogenesis in an insect egg (Euscelis plebejus). Wilhelm Roux's Archives 184:143–153

    Google Scholar 

  • Slegers H, Mettrie R, Kondo M (1977) Evidence for a cytoplasmic translational inhibitor RNA inArtemia salina gastrula embryos. FEBS. Lett 80:390–394

    PubMed  Google Scholar 

  • Spirin AS (1966) On ‘masked’ forms of messenger RNA in early embryogenesis and in other differentiating systems. Curr Top Dev Biol 1:1–38

    PubMed  Google Scholar 

  • Spirin AS, (1979) Messenger ribonucleoproteins (Informosomes) and RNA-binding proteins. Mol Biol Rep 5:53–57

    PubMed  Google Scholar 

  • Warn R, Bullard B, Moleki S (1979) Myosin as, a constituent of theDrosophila egg cortex. Nature (London) 278:651–653

    Google Scholar 

  • Winter H (1974) Ribonukleoprotein-Partikel aus dem teleotrophmeroistischen Ovar vonDysdercus intermedius Dist. (Heteroptera, Pyrrhoc.) und ihr Verhalten im zellfreien Proteinsynthesesystem. Wilhelm Roux's Archives 175:103–127

    Google Scholar 

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Author notes

  1. Herbert Jäckle

    Present address: European Molecular Biology Laboratory, P.O. 10.2209, D-6900, Heidelberg, FRG

Authors and Affiliations

  1. Department of Zoology, University of Texas at Austin, 78712, Austin, Texas, USA

    Herbert Jäckle

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  1. Herbert Jäckle
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Jäckle, H. Actin messenger in maternal RNP particles from an insect embryo (Smittia spec., Chironomidae, Diptera). Wilhelm Roux' Archiv 188, 225–233 (1980). https://doi.org/10.1007/BF00849052

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  • DOI: https://doi.org/10.1007/BF00849052

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