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Cytophotometric evidence for cell ‘transdifferentiation’ in planarian regeneration

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Summary

The role and fate of male germ cells in planarian regeneration was studied in a population ofDugesia lugubris s.1. which provided a suitable karyological marker to distinguish diploid male germ cells from triploid embryonic and somatic cells. The nuclear Feulgen-DNA content in non-replicating triploid muscle cells of the pharynx and in non-replicating male gonia of testes from intact animals were measured by the cytophotometric technique. The pharynx was then removed by transection and each anterior regenerant was allowed to completely regenerate this organ. Measurements of the Feulgen-DNA content in muscle cells of the regenerated pharynx showed that most of these cells (∼95%) have a DNA content typical of triploid cells; however, some muscle cells (∼5%) with a nuclear DNA content typical of male gonia alone were observed.

These results were interpreted in the following way. After transection, young male germ cells move from the testes to the wound where they participate in blastema formation along with reserve and/or somatic dedifferentiated cells. During regeneration some of these cells of male origin differentiate into pharyngeal muscle cells. Our findings are discussed in relation to the occurrence of mataplasia in planarians.

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References

  • Baguna J (1975a) Mitosis in the intact and regenerating planarianDugesia mediterranea n.sp. I. Mitotic studies during growth, feeding and starvation. J Exp Zool 195:53–64

    Google Scholar 

  • Baguna J (1975b) Mitosis in the intact and regenerating planarianDugesia mediterranea n.sp. II Mitotic studies during regeneration and a possible mechanism of blastema formation. J Exp Zool 195:65–79

    Google Scholar 

  • Banchetti R, Gremigni V (1973) Indirect evidence for neoblast migration and for gametogonia dedifferentiation in ex-fissiparous specimens ofDuggesia gonocephala s.l. Accad Naz Lincei 55:107–115

    Google Scholar 

  • Bedi KS, Goldstein DJ (1976) Cytophotometric factors causing apparent differences between Feulgen-DNA contents of different leukocyte types. Nature 251:439–440

    Google Scholar 

  • Benazzi M (1957) Cariologia diDugesia lugubris (O. Schmidt) Tricladida Paludicola. Caryologia 10:276–303

    Google Scholar 

  • Best JB, Hand S, Rosenvold R (1968) Mitosis in normal and regenerating planarians. J Exp Zool 168:157–168

    Google Scholar 

  • Betchaku T (1970) The cellular mechanism of formation of a regeneration blastema of fresh-water planariaDugesia dorotocephala I The behaviour of cells in a tiny body fragment isolated in vitro. J Exp Zool 174:253–280

    Google Scholar 

  • Bouvin A, Vendrely R, Vendrely C (1948) L'acide désoxiribonucléique du noyau cellulaire dépositaire des caractéres hèrèditaires arguments d'ordre analitìque. CR Acad Sci (Paris) 226:1061–1063

    Google Scholar 

  • Bowen D, Ryder TA (1973) The fine structure of the planarianPolycelis tenuis Iijima. I The pharynx. Protoplasma 78:223–241

    Google Scholar 

  • Brøndsted HV (1969) Planarian regeneration. Pergamon Press, Oxford

    Google Scholar 

  • Chandebois R (1976) Histogenesis and morphogenesis in planarian regeneration. S Karger, Basel

    Google Scholar 

  • Child CM (1921) Some considerations concerning the nature and origin of physiological gradients. Biol Bull 39:147–187

    Google Scholar 

  • Clayton RM (1975) Failure of growth regulation of the lens epithelium in strains of fast growing chicks. Genet Res 25:70–83

    Google Scholar 

  • Coward SJ (1969) Regeneration in planarians: some unresolved problems and questions. J Biol Psychol 11:15–19

    Google Scholar 

  • D'Amato F (1977) Nuclear cytology in relation to development. University Press, Cambridge

    Google Scholar 

  • Dubois F (1949) Contribution à l'étude de la migration des cellules de régénération chez les planaires dulcicoles Bull Biol 83:213–283

    Google Scholar 

  • Fedecka-Bruner B (1965) Régénération des testicules des planaires après destruction par le rayons X. In: Kiortsis V, Trampush H (eds) Regeneration in animals and related problem. North Holland, Amsterdam, pp 185–192

    Google Scholar 

  • Fedecka-Brumer B (1967) Etudes sur la régénération des organs génitaux chez la planaireDugesia lugubris. I Régénération des testicules après destruction. Bull Biol Fr 101:255–319

    Google Scholar 

  • Franquinet R, Lender Th (1973) Etude ultrastructurale des testicules dePolycelis nigra (Planaires). Evolution des cellules germinales mâles avant la spermiogenèse. Z Mikrosk Anat Forsch 87:4–22

    Google Scholar 

  • Gabriel A (1970) Etude morphologique et evolution biochimique des neoblastes au cours des premières phases de la régénération des planaires d'eau douce. Ann Embryol Morphogen 3:49–69

    Google Scholar 

  • Gremigni V (1974) The origin and cytodifferentiation of germ cells in the planarians. Boll Zool 41:359–377

    Google Scholar 

  • Gremigni V, Miceli C, Puccinelli I (1980a) On the role of germ cells in planarian regeneration: LA karyological investigation. J Embryol Exp Morphol 55:53–63

    Google Scholar 

  • Gremigni V, Miceli C, Picano E (1980b) On the role of germ cells in planarian regeneration: II Cytophotometric analysis of the nuclear Feulgen-DNA content in cells of regenerated somatic tissues. J Embryol Exp Morphol 55:65–76

    Google Scholar 

  • Gremigni V, Puccinelli I (1977) A contribution to the problem of the origin of blastema cells in planarians: a karyological and ultrastructural investigation. J Exp Zool 199:57–72

    Google Scholar 

  • Hay ED (1968) Dedifferentiation and Metaplasia in vertebrate and invertebrare regeneration. In: Ursprung H (ed) The stability of differentiate state. Springer, Berlin, Heidelberg, New York, pp 85–108

    Google Scholar 

  • Hay ED, Coward SJ (1975) Fine structure studies on the planarianDugesia. I Nature of the ‘neoblast” and other cell types in noninjured worms. J Ultrastruct Res 50:1–21

    Google Scholar 

  • Hyman L (1951) The invertebrates. Mc Graw-Hill, New York

    Google Scholar 

  • Itikawa O, Ogura Y (1954) Simplified manifacture and histochemical use of the Schiff reagent. Stain Technol 29:9–11

    Google Scholar 

  • Kiortsis V, Trampush H (1965) Regeneration in animals and related problems. North-Holland, Amsterdam

    Google Scholar 

  • Lang A (1912) Über Regeneration bei Planarien. Arch Mikrosk Anat 79:361–426

    Google Scholar 

  • Lange CS (1967) A quantitative study of the number and distribution of neoblasts inDugesia lugubris (Planaria) with reference to size and ploidy. J Embryol Exp Morphol 18:199–213

    Google Scholar 

  • Lender Th (1962) Factors in morphogenesis of regenerating freshwater planaria. Adv Morphog 2:305–331

    Google Scholar 

  • Mac Rae E (1963) Observations on the fine structure of pharyngeal muscle in the planariaDugesia tigrina. J Cell Biol 18:651–662

    Google Scholar 

  • Mirsky AE, Ris H (1949) Variable and constant components of chromosomes. Nature 163:666–667

    Google Scholar 

  • Pedersen KJ (1972) Studies on regeneration blastemas of the planarianDugesia tigrina with special reference to differentiation of the muscle-connective tissue filament system. Wilhelm Roux' Arch Entwicklungsmech Org 169:134–169

    Google Scholar 

  • Pritchard DJ, Clayton RM, De Pomerai DI (1978) Transdifferentiation of chicken neural retina into lens and pigment eptihelium in culture: controlling influences. J Embryol Exp Morphol 48:1–21

    Google Scholar 

  • Puccinelli I, Gremigni V (1977) Sulla presenza nei blastemi di planarie de cellule provenienti dal territorio gonadico femminile. Accad Naz Lincei 63:588–592

    Google Scholar 

  • Reinert J (1973) Aspects of organization. Organogenesis and embryogenesis. In: Street HE (ed.) Plant tissue and Cell Culture. Blackwell Sci Publ, Oxford, pp 338–355

    Google Scholar 

  • Rose C, Shostak S (1968) The transformation of gastrodermal cells to neoblasts in regeneratingPhagocata gracilis (Leidy). Exp Cell Res 50:553–561

    Google Scholar 

  • Steinmann P (1925) Das Verhalten der Zellen und Gewebe im regenerierenden Tricladenkörper. Verh Naturforsch Ges 36:133–162

    Google Scholar 

  • Trampush HAL, Harrebomée AE (1969) Dedifferentiation and interconvertibility of the different cell-types in the amphibian extremity. Acta Embryol Exp 35–69

  • Vasil IK, Vasil V (1972) Totipotency and embryogenesis in plant cell and tissue cultures. In Vitro 8:117–127

    Google Scholar 

  • Wolff E (1962) Recent researches on the regeneration of Planaria. In: Rudnick D (ed) Regeneration 20th Growth Symposium. The Ronald Press, New York, pp 53–84

    Google Scholar 

  • Wolff E, Dubois F (1947a) La migration des cellules de régénération et les facteurs qui la provoquent chez les Triclades. CR Acad Sci 224:1387–1388

    Google Scholar 

  • Wolff E, Dubois F (1947b) Sur une methode d'irradiation localisée permettant de mettre en évidence la migration des cellules de régénération chez les planaires. CR Soc Biol 141:903–906

    Google Scholar 

  • Wolff E, Lender Th, Ziller-Sengel C (1964) Le rôle de facterus auto-inhibiteurs dans la régénération des Planaires. Rev Suisse Zool 71:75–98

    Google Scholar 

  • Woodruff L, Burnett L (1965) Theorigin of blastema inDugesia tigrina. Exp Cell Res 38:295–305

    Google Scholar 

  • Yamada T (1976) Dedifferentiation associated with cell-type conversion in the newt lens regenerating system: a review. In: Müller-Bérat N, et al. (ed) Progress in differentiation research. North-Holland, Amsterdam, pp 355–360

    Google Scholar 

  • Yamada T (1977) Control mechanism in cell-type conversion in newt lens regeneration. S. Karger, Basel

    Google Scholar 

Download references

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Author notes

  1. Cristina Miceli

    Present address: Istituto di Zoologia, Università di Camerino, Italia

Authors and Affiliations

  1. Instituto di Zoologia e Anatomia Comparata, Università di Pisa, via Volta 4, 1-56100, Pisa, Italia

    Vittorio Gremigni & Cristina Miceli

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  1. Vittorio Gremigni
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  2. Cristina Miceli
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Gremigni, V., Miceli, C. Cytophotometric evidence for cell ‘transdifferentiation’ in planarian regeneration. Wilhelm Roux' Archiv 188, 107–113 (1980). https://doi.org/10.1007/BF00848801

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