Advertisement

Evagination of imaginal discs in the fleshflySarcophaga crassipalpis: Hormonal control in vivo

  • P. Sivasubramanian
Article

Summary

The hormonal control of evagination of the imaginal leg discs of the fleshflySarcophaga crassipalpis was studied by means of ectopic transplantation of discs from mature larvae or prepupae onto prepupal hosts. Evagination was influenced by the age of the donor and the developmental commitment of the host. Discs from non-diapuse as well as diapause-committed donors did not differ in their capacity to evaginate and differentiate. Mature larval discs from either type of donor could evaginate only when transplanted onto non-diapause hosts; they failed to evaginate on diapause-committed hosts even though host discs evaginated in situ about 40 h after transplantation. Discs from white prepupae evaginated whether transplanted onto non-diapause or onto diapause-committed hosts.

Key words

Imaginal discs Evagination Sarcophaga crassipalpis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bhaskaran, G., Sivasubramanian, P.: Metamorphosis of imaginal disks of the housefly: Evagination of transplanted disks. J. Exp. Zool.171, 385–396 (1969)Google Scholar
  2. Chihara, C.J., Petri, W.H., Fristrom, J.W., King, D.S.: The assay of ecdysones and juvenile hormones onDrosophila imaginal disks in vitro. J. Insect Physiol.18, 1115–1123 (1972)Google Scholar
  3. Denlinger, D.L.: Embryonic determination of pupal diapause in the fleshfly,Sarcophaga crassipalpis. J. Insect Physiol.17, 1815–1822 (1971)Google Scholar
  4. Denlinger, D.L.: Induction and termination of pupal diapause inSarcophaga (Diptera: Sarcophagidae). Biol. Bull.142, 11–24 (1972)Google Scholar
  5. Ephrussi, B., Beadle, G.W.: A technique of transplantation forDrosophila. Amer. Natural.70, 218–225 (1936)Google Scholar
  6. Fraenkel, G., Hsiao, C.: Manifestation of pupal diapause in two species of flies,Sarcophaga argyrostoma andS. bullata. J. Insect Physiol.14, 689–705 (1968a)Google Scholar
  7. Fraenkel, G., Hsiao, C.: Morphological and endocrinological aspects of pupal diapause in a fleshfly,Sarcophaga argyrostoma. J. Insect Physiol.14, 707–718 (1968b)Google Scholar
  8. Fristrom, J.W., Logan, W.R., Murphy, C.: The synthetic and minimal culture requirements for evagination of imaginal discs ofDrosophila melanogaster in vitro. Develop. Biol.33, 441–456 (1973)Google Scholar
  9. Gibbs, D.: The initiation of adult development inSarcophaga argyrostoma by β-ecdysone. J. Insect Physiol.22, 1195–1200 (1976)Google Scholar
  10. Mandaron, P.: Sur le méchanisme de l'évagination des disques imaginaux chez la Drosophile. Develop. Biol.25, 581–605 (1971)Google Scholar
  11. Mandaron, P.: Effects of α-ecdysone, β-ecdysone and inokosterone on the in vitro evagination ofDrosophila leg discs and the subsequent differentiation of imaginal integumentary structures. Develop. Biol.31, 101–113 (1973)Google Scholar
  12. Milner, M.J., Sang, J.H.: Relative activities of α-ecdysone and β-ecdysone for the differentiation in vitro ofDrosophila melanogaster imaginal discs. Cell.3, 141–143 (1974)Google Scholar
  13. Ohmori, K., Ohtaki, T.: Effects of ecdysone analogues on development and metabolic activity of wing disks of the fleshfly,Sarcophaga peregrina in vitro. J. Insect Physiol.19, 1199–1210 (1973)Google Scholar
  14. Ohtaki, T., Takahashi, M.: Induction and termination of pupal diapause in relation to the change of ecdysone titer in the fleshfly,Sarcophaga peregrina. Jap. J. Med. Sci. Biol.25, 369–376 (1972)Google Scholar
  15. Postlethwait, J.H., Schneiderman, H.A.: Induction of metamorphosis by ecdysone analogues:Drosophila imaginal disks cultured in vivo. Biol. Bull.138, 47–55 (1970)Google Scholar
  16. Shaaya, E., Karlson, P.: Der Ecdysontiter während der Insektenentwicklung. II. Die postembryonale Entwicklung der SchmeißfliegeCalliphora erythrocephala Meig. J. Insect Physiol.11, 65–69 (1965)Google Scholar
  17. Zdarek, J., Denlinger, D.L.: Action of ecdysoids, juvenoids and non-hormonal agents on termination of pupal diapause in the fleshfly. J. Insect Physiol.21, 1193–1202 (1975)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • P. Sivasubramanian
    • 1
  1. 1.Department of BiologyUniversity of New BrunswickFrederictonCanada

Personalised recommendations