The role of the peripodial membrane in the morphogenesis of the eye-antennal disc ofDrosophila melanogaster

  • Martin John Milner
  • Alison Jane Bleasby
  • Andrew Pyott


The early morphogenesis of the eye-antennal disc ofDrosophila in response to 20-hydroxy ecdysone involves the curling of the eye anlagen dorsally over the antenna. During this process, the area of the peripodial membrane is substantially reduced. The peripodial membrane is taut at this stage, and if it is cut the curling of the disc cannot continue, and the eye anlagen returns to its original position within one minute of the operation. In contrast, cutting the columnar epithelium between the eye and antennal anlagen does not disrupt curling, but actually facilitates it. During curling, the cells of the peripodial membrane appear healthy, and exhibit basal extensions. We suggest that the curling of the eye is mediated by the conversion of cuboidal peripodial membrane cells into pseudostratified columnar epithelium at the edges of the peripodial membrane. Subsequently, cells of the peripodial membrane secrete first a pupal cuticle, and then an imaginal cuticle.

Key words

Drosophila Imaginal disc Morphogenesis Tissue culture 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bryant PJ (1975) Pattern formation in the imaginal wing disc ofDrosophila melanogaster: Fate map, regeneration and duplication J Exp Zool 193:49–78Google Scholar
  2. Bryant PJ (1978) Pattern formation in imaginal discs. In: Ashburner M, wright TRF (eds) The genetics and biology ofDrosophila. Academic Press, New York and London, pp 229–335Google Scholar
  3. Deak II (1980) A model linking segmentation, compartmentalization and regeneration inDrosophila development. J Theor Biol 84:477–504Google Scholar
  4. Edwards JS, Milner MJ, Chen SW (1978) Integument and sensory nerve differentiation ofDrosophila leg and wing imaginal discs in vitro. Wilhelm Roux's Arch 185:59–77Google Scholar
  5. Gottschewski GHM (1960) Morphogenetische Untersuchungen an in vitro wachsenden Augenanlagen vonDrosophila melanogaster. Wilhelm Roux's Arch 152:204–229Google Scholar
  6. Haynie JL (1975) Ph.D. Thesis, University of California, IrvineGoogle Scholar
  7. Karpen GH, Schubiger G (1981) Extensive regulatory capabilities of aDrosophila imaginal disk blastema. Nature 294:744–747Google Scholar
  8. Kumar K, Ouweneel WJ, Faber J (1979) Differentiation capacities of the labial imaginal disc ofDrosophila melanogaster. Wilhelm Roux's Arch 186:51–64Google Scholar
  9. Locke M (1981) Cell structure during insect metamorphosis. In: Gilbert LI, Frieden E (eds) Metamorphosis: a problem in developmental biology. Plenum Press, New York, pp 75–103Google Scholar
  10. Locke M, Huie P (1981) Epidermal feet in pupal segment morphogenesis. Tissue Cell 13:7870803Google Scholar
  11. Milner MJ (1980) Epithelial and pattern integration inDrosophila eye-antennal imaginal discs cultured in vitro. In: Kurstak E, Maramorosch K, Dübendorfer A (eds) Invertebrate systems in vitro. Elsevier/North Holland Biomedical Press Amsterdam-Oxford-New York pp 135–148Google Scholar
  12. Milner MJ, Haynie JL (1979) Fusion ofDrosophila eye-antennal imaginal discs during differentiation in vitro. Wilhelm Roux's Arch 185:363–370Google Scholar
  13. Milner MJ, Sang JH (1974) Relative activities of α-ecdysone and β-ecdysone for the differentiation in vitro ofDrosophila melanogaster imaginal discs. Cell 3:141–143Google Scholar
  14. Ouweneel WJ (1970) Developmental capacities of young and mature, wild-type and opht eye imaginal discs inDrosophila melanogaster. Wilhelm Roux's Arch 166:78–88Google Scholar
  15. Poodry CA, Schneiderman HA (1970) The ultrastructure of the developing leg ofDrosophila melanogaster. Wilhelm Roux's Arch 166:1–44Google Scholar
  16. Ready DF, Hanson TE, Benzer S (1976) Development of theDrosophila retina, a neurocrystalline lattice. Dev Biol 53:217–240Google Scholar
  17. Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212Google Scholar
  18. Robertson CW (1936) The metamorphosis ofDrosophila melanogaster, including an accurately timed account of the principle morphological changes. J Morphol 59:351–399Google Scholar
  19. Schneider I (1964) Differentiation of larvalDrosophila eye-antennal discs in vitro. J Exp Zool 156:91–104Google Scholar
  20. Schneider I (1966) Histology of larval eye-antennal disks and cephalic ganglia ofDrosophila cultured in vitro. J Embryol Exp Morphol 15:271–279Google Scholar
  21. Shields G, Sang JH (1977) Improved medium for culture ofDrosophila embryonic cells. Drosophila Inform Serv 52:161Google Scholar
  22. Sprey TE (1970) Morphological and histochemical changes during the development of some of the imaginal disks ofCalliphora erythrocephala. Neth J Zool 20:253–275Google Scholar
  23. Sprey TE, Oldenhave M (1974) A detailed organ map of the wing disk ofCalliphora erythrocephala. Neth J Zool 24:291–310Google Scholar
  24. Tucker JB (1967) Changes in nuclear structure during binary fission in the ciliateNassula. J Cell Sci 2:481–498Google Scholar
  25. Ursprung H (1972) The fine structure of imaginal discs. In: Ursprung H, Nöthiger R (eds) The biology of imaginal discs. Springer, Berlin Heidelberg New York, pp 93–107Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Martin John Milner
    • 1
  • Alison Jane Bleasby
    • 1
  • Andrew Pyott
    • 1
  1. 1.Department of ZoologyThe UniversitySt. AndrewsUK

Personalised recommendations