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Influence of a haemolymph protein fraction on the binding of juvenile hormone in homogenates of insect epidermis (Plodia interpunctella (Hübner))

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Summary

A carrier protein fraction (CPF) from larval haemolymph was found to influence binding and catabolism of tritiated juvenile hormone (JH) in homogenates of larval epidermis. The CPF reduced binding of tritiated JH in all of the particulate fractions but did not alter the relative binding pattern when compared with JH alone. The CPF also protected the hormone from degradative enzymes in the membrane vesicle and microsomal + cytosol fractions but not in the nuclear and mitochondrial fractions. Preliminary evidence exists for high-affinity binding sites for JH in the nuclear and mitochondrial fractions. We conclude that the CPF influences catabolism of the tritiated JH but does not participate in subcellular recognition of JH in homogenized target tissue.

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Authors and Affiliations

  1. Insect Attractants, Behavior, and Basic Biology Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 32604, Gainesville, Florida, USA

    S. M. Ferkovich & R. R. Rutter

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  1. S. M. Ferkovich
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  2. R. R. Rutter
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Ferkovich, S.M., Rutter, R.R. Influence of a haemolymph protein fraction on the binding of juvenile hormone in homogenates of insect epidermis (Plodia interpunctella (Hübner)). Wilhelm Roux' Archiv 179, 243–247 (1976). https://doi.org/10.1007/BF00848649

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  • DOI: https://doi.org/10.1007/BF00848649

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