Immunological analysis of hemoglobin transition during metamorphosis of normal and isogenicXenopus
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Antisera against larval and adultXenopus hemoglobins as well as adult human hemoglobin showed no cross-reaction when tested by immunodiffusion against each heterologous antigen. In this test hemoglobin of a single animal produced two precipitation lines for larvae, but only one for adult stages. Immunoelectrophoresis also revealed more complex precipitation patterns for larval than for adult hemoglobins. Hemoglobin of the isogenic hybrid cloneXenopus laevis/X. gilli also reacted with antisera against normalXenopus hemoglobin.
Quantitation of hemoglobins, analyzed by radial immunodiffusion showed fewer than 1% of adult hemoglobin in red cells of larvae, but 30% at completion of metamorphosis. Two weeks later adult hemoglobin attained over 90%, and in red cells of adultXenopus an average of 1% larval hemoglobin were detected.
The relatively short transition period suggests that the loss of larval hemoglobin may be due to the elimination of larval red cells, and that the increase in adult hemoglobin may be indicative of a new cell line.
Key wordsHemoglobin transition Immunoelectrophoresis Quantitative Immunodiffusion Metamorphosis Xenopus
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- Drabkin DL, Austin TH (1935) Spectrophotometric studies II. Preparations from washed blood cell: nitro oxide hemoglobin and sulfhemoglobin. J Biol Chem 112:51–65Google Scholar
- Duncan DB (1955) Multiple range and multipleF-tests. Biometrics 11:1–42Google Scholar
- Fey H, Pfister H, Messerli J, Sturzenegger N, Grolimund F (1976) Methods of isolation, purification and quantitation of bovine immunoglobulins. A technical review. Zentralbl Veterinaermed, Reihe B:23 269–300Google Scholar
- Forman LJ, Just JJ (1978) Cellular quantitation of hemoglobin transition during metamorphosis of the bullfrog,Rama catesbeiana. Am Zool 18:658Google Scholar
- Just JJ, Schwager J, Weber R (1977) Hemoglobin transition in relation to metamorphosis in normal and isogenicXenopus. Wilhelm Roux' Arch Entwicklungsmech Org 113:307–323Google Scholar
- Kobel HR, Du Pasquier L (1975) Production of large clones of histocompatible fully identical clawed toadsXenopus. Immunogenetics 2:87–91Google Scholar
- Maniatis GM, Ingram VM (1971) Erythropoiesis during Amphibian metamorphosis II. Immunochemical study of larval and adult hemoglobins ofRana catesbeiana. J Cell Biol 49:380–389Google Scholar
- Nieuwkoop PD, Faber J (1956) Normal Table ofXenopus laevis Daudin. North Holland, Amsterdam, pp 252Google Scholar
- Ouchterlony OE (1958) Diffusion-in-gel methods for immunological analyses. Prog Allergy 5:1–78; 6:30–154Google Scholar
- Sullivan B (1974) Amphibian hemoglobins In: Florkin M, Scheer BT (eds) Chemical zoology, Vol. 9. pp 77–122: Academic Press, New YorkGoogle Scholar