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Wilhelm Roux's archives of developmental biology

, Volume 188, Issue 1, pp 27–36 | Cite as

In vitro differentiation ofPieris brassicae imaginal wing discs: Effects and metabolism of ecdysone and ecdysterone

  • Catherine Blais
  • René Lafont
Article

Summary

Imaginal wing discs ofPieris brassicae can be cultured in vitro for extended periods. Their ultrastructural development was investigated after culture in the presence of various concentrations of ecdysone and ecdysterone. When ecdysone or low concentrations of ecdysterone (2×10−7 M) were added to culture media, larval discs secreted a pupal cuticle and they subsequently differentiated scales; prepupal discs completed their imaginal development. With a higher concentration of ecdysterone (2×10−6 M), all discs produced abundant but fragmentary cuticular material.

Prepupal discs were able to metabolize both hormones in vitro. Ecdysterone was mainly converted into a polar compound detectable after a short period of incubation. Ecdysone was transformed, at a slower rate, forming a polar compound and 26-hydroxyecdysone but no ecdysterone.

Key words

Imaginal discs Ecdysone Tissue culture In vitro 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Catherine Blais
    • 1
  • René Lafont
    • 1
  1. 1.Laboratoire de ZoologieEcole Normale SupérieureParis Cedex 05France

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