Advertisement

Transformation of avian myogenic cultures with myelocytomatosis virus strain 29

  • Christopher M. West
Rapid Communications

Summary

Primary cultures of proliferating chick presumptive myoblasts were exposed of either to two RNA tumor viruses and shortly thereafter treated with 5-bromodeoxyuridine (BUdR) to suppress differentiation. The effect of a Rous sarcoma virus which was temperature-sensitive for transformation (tsRSV) has been characterized previously and was used as a reference for evaluating the effect of a myelocytomatosis virus (MC29) and its helper. Two subcultures following exposure, both infected cultures were extensively transformed as indicated by cell morphology. Relaxation of the BUdR block at this time resulted in cultures which still appeared transformed and did not contain myoblast or myotube-like cells or two of their molecular markers. In contrast, uninfected controls and tsRSV-infected cultures which were shifted-up to the nonpermissive temperature produced numerous spontaneously contracting myotubes. The results confirm previous evidence that infection of presumptive myoblasts by tsRSV at the premissive temperature preserves the extant state of differentiation of presumptive myoblasts and suggest, by analogy, that MC29-infection renders a similar effect.

Key words

Myogenesis Transformation MC29 Rous sarcoma virus 5-Bromodeoxyuridine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Cohen R, Pacifici M, Rubinstein N, Biehl J, Holtzer H (1977) Effect of a tumor promoter on myogenesis. Nature 266:538–540Google Scholar
  2. Dientsman SR, Holtzer H (1977) Skeletal myogenesis. Control of proliferation in a normal cell lineage. Exp Cell Res 107:355–364Google Scholar
  3. Donner P, Greiser-Wilke I, Moelling K (1982) Nuclear localization and DNA binding of the transforming gene product of avian myelocytomatosis virus. Nature 296:262–266Google Scholar
  4. Durban EM, Boettiger D (1981) Differential effects of transforming avian RNA tumor viruses on avian macrophages. Proc Natl Acad Sci, USA 78:3600–3604Google Scholar
  5. Erikson RL, Purchio AF, Erikson E, Collett MS, Brugge JS (1980) Molecular events in cells transformed by Rous sarcoma virus J Cell Biol 87:319–325Google Scholar
  6. Fiszman, MY, Fuchs P (1975) Temperature-sensitive expression of differentiation in transformed myoblasts. Nature 254:429–431Google Scholar
  7. Fitzgerald TP, West CM (1983) The comparative effects of tumorrelated agents on developmental marker expression in presumptive myoblasts and myotubes. Exp Cell Res 144:83–93Google Scholar
  8. Holtzer H, Biehl J, Yeoh G, Meganathan R, Kaji A (1975) Effect of oncogenic virus on muscle differentiation. Proc Natl Acad Sci, USA 72:4051–4055Google Scholar
  9. Holtzer H, Biehl J, Pacifici M, Boettiger D Payette B, West C (1980a) The effects of temperature-sensitive Rous sarcoma virus mutants and phorbol diester tumor promoters on cell lineages. In: McKinnell, RG et al. (eds) Differentiation and neoplasia: Results and problems in differentiation, vol 11. Springer, Berlin Heidelberg New York, pp 166–177Google Scholar
  10. Holtzer H, Croop J, Toyama Y, Bennett G, Fellini S, West C (1980b) Differences in differentiation programs between presumptive myoblasts and their daughters, the definitive myoblasts and myotubes. In: Pette D (ed) Plasticity of muscle. de Gruyter & Co., New York, pp 133–146Google Scholar
  11. Kaufman SJ, Marks CM, Bohn J, Faiman LE (1980) Transformation is an alternative to normal skeletal muscle development. Exp Cell. Res 125:333–349Google Scholar
  12. Moscovici C, Gazzolo L (1982) Transformation of hemopoietic cells with avian leukemia viruses. In: Klein, G (ed) Advances in viral oncology. Raven Press, New York, pp 83–106Google Scholar
  13. O'Conner CM, Balzer DR, Lazarides E (1979) Phosphorylation of subunit proteins of intermediate filaments from chicken muscle and nonmuscle cells. Proc Natl Acad Sci, USA 76:819–823Google Scholar
  14. Ramsay G, Hayman MJ (1982) Isolation and biochemical chameterization of partially transformation-defective mutants of avian myelocytomatosis virus strain MC29: localization of the mutation to the myc domain of the 110,000-dalton gag-myc polyprotein. J Virol 41:745–753Google Scholar
  15. Stockdale F, Okazaki K, Nameroff M, Holtzer H (1964) 5-bromodeoxyuridine: effect on myogenesis in vitro. Science 146:533–535Google Scholar
  16. Toyama Y, West CM, Holtzer H (1979) Differential response of myofibrils and 10-NM filaments to a cocarcinogen. Am J Anat 156:131–137Google Scholar
  17. West CM, Holtzer H (1982) Protein synthesis and degradation in cultured muscle is altered by a phorbol diester tumor promoter. Arch Biochem Biophys 219:335–350Google Scholar
  18. West CM, Boettiger, D (1983) Selective effect of Rous sarcoma virus src gene expression on contractile protein synthesis in chick embryo myotubes. Cancer Res 43:2042–2046Google Scholar
  19. Zeller NK, Gazzolo L, Moscovici C (1980) A study of the epithelioid transformation of MC29-infected chicken embryo cells. Virology 104:239–24Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Christopher M. West
    • 1
  1. 1.Department of AnatomyUniversity of Florida College of MedicineGainesvilleUSA

Personalised recommendations