Wilhelm Roux's archives of developmental biology

, Volume 189, Issue 1, pp 17–24 | Cite as

Characterization by isoelectric focusing of chorion protein variants inDrosophila melanogaster and their use in developmental and linkage analysis

  • Claudine Zighera Yannoni
  • William H. Petri


Drosophila melanogaster chorion proteins are characterized on one-dimensional isoelectric focusing (IF) gels. The six major chorion components previously identified on SDS gels are shown to resolve into at least 11 components in our IF system. IF screening of 102 geographic strains ofDrosophila melanogaster revealed seven cases of variation in major chorion components. Two strains, Crimea and Falsterbo, which were monomorphic for a variant B1 protein and two strains, Skafto and Lausanne, which were monomorphic for a variant C1 protein, were chosen for further study. After IF developmental analysis of F1 hybrids had indicated that the sources of the variation resided in the structural genes for these proteins, each variant was crossed to a multiply marked and inverted strain (BLT) to determine the linkage group of the variant gene. To localize genes to more specific sites multiply marked 3rd (SKERO) or X-chromosomal (CB1) (X-PLE) mapping strains were used. In both Crimea and Falsterbo the gene for the B1 protein is located near map location 26 on the 3rd chromosome. In both Lausanne and Skafto the C1 gene is located on the X chromosome. Hence, for the first time, we have demonstrated genetically the non-linkage of two chorion genes, B1 and C1.

Key words

Drosophila Geographic strains Chorion proteins Electrophoretic variants Chorion gene linkage 


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  1. Bonner WM, Laskey RA (1974) A film detection method for tritiumlabeled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem 46:83–88Google Scholar
  2. Bregliano JC, Picard G, Bucheton A, Pelisson A, Lavige JM, L'Heritier P (1980) Hybrid Dysgenesis inDrosophila melanogaster. Science 207:606–611Google Scholar
  3. Deisseroth A, Nienhuis A, Lawrence J, Giles R, Turner P, Ruddle FH (1978) Chromosomal localization of human β-globin gene on chromosome 11 in somatic cell hybrids. Proc Natl Acad Sci USA 75:1456–1460Google Scholar
  4. Deisseroth A, Nienhuis A, Turner P, Velez R, Anderson WF, Ruddle FH, Lawrence J, Creagan R, Kucherlapah R (1977) Localization of the human α-globin structural gene to chromosome 16 in somatic cells hybrids by molecular hybridization assay. Cell 12:205–218Google Scholar
  5. Engels WR (1979) Extrachromosomal control of mutability inDrosophila melanogaster. Proc Natl Acad Sci USA 76:4011–4015Google Scholar
  6. Ephrussi B, Beadle GW (1936) A technique of transplantation forDrosophila. Am Nat 70:218–225Google Scholar
  7. Goldsmith MR, Basehoar G (1978) Organization of the chorion genes ofBombyx mori. A multigene family. I. Evidence for linkage to chromosome 2. Genetics 90:291–310Google Scholar
  8. Hughes SH, Stubblefield E, Paynar F, Engel JD, Dodgson JB, Spector D, Cordell B, Schimke RT, Varmus HE (1979) Gene localization by chromosome fractionation: Globin genes are on at least two chromosomes and three estrogen-inducible genes are on three chromosomes. Proc Natl Acad Sci USA 76:1348–1352Google Scholar
  9. Kafatos FC, Regier J, Mazur G, Nadel M, Blau H, Petri WH, Wyman AR, Gelinas R, Moore P, Paul M, Efstratiadis A, Vournakis J, Goldsmith M, Hunsley J, Baker B, Nardi J (1977) The eggshell of insects: Differentiation-specific proteins and the control of their synthesis and accumulation during development. In: Beermann W (ed) Results and problems in cell differentiation, vol 8. Springer, Berlin Heidelberg New York, pp 45–145Google Scholar
  10. Kidwell MG, Kidwell JF, Sued JA (1977) Hybrid dysgenesis inDrosophila melanogaster: A syndrome of aberrant traits including mutation, sterility and male recombination. Genetics 86:813–833Google Scholar
  11. King RC (1970) Ovarian Development inDrosophila melanogaster. Academic Press, New YorkGoogle Scholar
  12. Lindsley DL, Grell EH (1967) Genetic Variations ofDrosophila melanogaster. Carnegie Inst WashingtonGoogle Scholar
  13. Lucchesi JC, Suzuki DT (1968) The interchromosomal control of recombination. Annu Rev Genet 2:53–86Google Scholar
  14. Margaritis LH, Kafatos FC, Petri WH (1980) The eggshell ofDrosophila melanogaster: I. Fine structure of the layers and regions of the wildtype eggshell. J Cell Sci in pressGoogle Scholar
  15. O'Farrell PZ, Goodman HM, O'Farrell PH (1977) High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12:1133–1142Google Scholar
  16. Petri WH, Mindrinos MN, Lombard ML, Margaritis LH (1979) In vitro development of theDrosophila chorion in a chemically defined organ culture medium. Wilhelm Roux's Archives 186:351–362Google Scholar
  17. Petri WH, Wyman AR, Kafatos FC (1976) Specific protein synthesis in cellular differentiation: III. The eggshell proteins ofDrosophila melanogaster and their program of synthesis. Dev Biol 49:185–199Google Scholar
  18. Picard G, Bregliano JC, Bucheton A, Lavige JM, Pelisson A, Kidwell MG (1978) Non-Mendelian female sterility and hybrid dysgenesis inDrosophila melanogaster. Genet Res Cambridge 32:275–287Google Scholar
  19. Robb JA (1969) Maintenance of imaginal discs ofDrosophila melanogaster in chemically defined media. J Cell Biol 41:876–885Google Scholar
  20. Smith EW, Torbert JV (1958) Study of two abnormal hemoglobins with evidence for a new genetic locus for hemoglobin information. Bull Johns Hopkins Hosp 102:38–45Google Scholar
  21. Spradling AC, Mahowald AP (1979) Identification and genetic localization of mRNAs from ovarian follicle cells ofDrosophila melanogaster. Cell 16:589–598Google Scholar
  22. Spradling AC, Waring GL, Mahowald AP (1979) Drosophila bearing theocelliless mutation underproduce two major chorion proteins both of which map near this gene. Cell 16:609–616Google Scholar
  23. Thireos G, Shea RG, Kafatos FC (1979) Identification of chorion protein precursors and the mRNAs that encode them inDrosophila melanogaster. Proc Natl Acad Sci USA 76:6279–6283Google Scholar
  24. Thompson JN, Woodruff RC (1978) Mutator genes-pacemakers of evolution. Nature 274:317–321Google Scholar
  25. Waring GL, Mahowald AP (1979) Identification and time of synthesis of chorion proteins inDrosophila melanogaster. Cell 16:599–607Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Claudine Zighera Yannoni
    • 1
  • William H. Petri
    • 1
  1. 1.Biology DepartmentBoston CollegeChestnut HillUSA

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