Expression of immunoreactive myosin and myoglobin in the developing chicken gizzard

  • Dieter Stuewer
  • Ute Gröschel-Stewart
Article

Summary

Antibodies to adult-type myosin and myoglobin from chicken gizzard were used to study the expression of these proteins during chicken embryogenesis. Using the indirect immunofluorescent technique, myosin was detected as discrete fluorescent foci in the central part of the presumptive chicken gizzard as early as day 5 of development. During the following days, immunoreactive myosin extends both craniocaudally as well as laterally and reaches the serosal and luminal borders by day 13/14. On day 16, the adult fascicular pattern is achieved. As judged by enzymelinked immunoassay and spectroscopic methods, myoglobin did not appear until day 18.

Key words

Chicken gizzard Embryogenesis Myosin Myoglobin Immunoassay 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Dieter Stuewer
    • 1
  • Ute Gröschel-Stewart
    • 1
  1. 1.Institut für ZoologieTechnische HochschuleDarmstadtFederal Republic of Germany

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