Advertisement

Changes in the differentiation tendencies of the hypoblast-free Hensen's node during “Gastrulation” in the chick embryo

  • Marianne Veini
  • K. Hara
Article

Summary

Hensen's node was isolated from chick blastoderms of medium-streak to headfold stages from which the endodermal layer had previously been removed. The isolates were culturedin vivo by means of the intra-coelomic grafting technique. Node pieces with the endodermal layer intact served as controls.

Endodermal differentiation tendencies gradually decreased from the medium-streak to the pre-head-process stage and completely disappeared at the head-process stage, whereas the controls gave rise to endodermal structures throughout all stages. “Cranial” structures such as oesophagus and trachea, often together with thyroid, parathyroid and/or thymus, were only found in grafts of younger stages, while gizzard, intestine and/or pancreas were observed in many gut-containing grafts throughout all stages.

There was a constant high incidence of notochord, muscle, and cartilage formation. The incidence of mesonephric structures, sometimes accompanied by adrenal gland, rose steadily throughout all stages both in experimentals and controls.

Neural differentiation tendencies (rhombencephalon and/or spinal cord) were always present in the nodes isolated (with or without endoderm) from the definitive primitive-streak stage onwards, but in nodes from earlier stages the incidence of neural differentiation was significantly lower.

The results are discussed in relation to the possible location and determination of the prospective endoderm and mesoderm.

Keywords

Spinal Cord Developmental Biology Adrenal Gland Chick Embryo Neural Differentiation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Gallera, J.: Excision et transplantation des différentes régions de la ligne primitive chez le Poulet. C. R. Ass. Anat. 49 Réunion, Madrid 1964. In: Bull. Ass. Anat. No125, 632–639 (1965)Google Scholar
  2. Grabowski, C. T.: The effects of the excision of Hensen's node on the early development of the chick embryo. J. exp. Zool.133, 301–344 (1956)Google Scholar
  3. Hamburger, V.: Morphogenetic and axial self-differentation of transplanted limb primordia of 2-day chick embryos. J. exp. Zool.77, 379–399 (1938)Google Scholar
  4. Hamburger, V.: A manual of experimental embryology. Chicago: Univ. Chicago Press 1960Google Scholar
  5. Hamburger, V., Hamilton, H. L.: A series of normal stages in the development of the chick embryo. J. Morph.88, 49–92 (1951)Google Scholar
  6. Hara, K.: Regional neural differentiation induced by prechordal and presumptive chordal mesoderm in the chick embryo. Utrecht: Thesis 1961Google Scholar
  7. Hara, K.: “Dark-field” illumination for micro-surgical operations on chick blastodermsin vitro. Mikroskopie26, 61–63 (1970)PubMedGoogle Scholar
  8. Hara, K.: Micro-surgical operation on the chick embryoin ovo without vital staining—A modification of the intra-coelomic grafting technique. Mikroskopie27, 267–270 (1971)PubMedGoogle Scholar
  9. Hunt, T. E.: The development of gut and its derivatives from the mesectoderm and mesentoderm of early chick blastoderms. Anat. Rec.68, 349–369 (1937a)Google Scholar
  10. Hunt, T. E.: The origin of entodermal cells from the primitive streak of the chick embryo. Anat. Rec.68, 449–459 (1937b)Google Scholar
  11. Modak, S. P.: Analyse expérimentale de l'origine de l'endoblaste embryonnaire chez les oiseaux. Rev. suisse Zool.73, 877–908 (1966)PubMedGoogle Scholar
  12. Nicolet, G.: Étude autoradiographique de la destination des cellules invaginées au niveau du noeud de Hensen de la ligne primitive achevée de l'embryon de Poulet. Acta Embryol. Morph. exp.8, 213–220 (1965)Google Scholar
  13. Nicolet, G.: Analyse autoradiographique de la localisation des différentes ébauches présomptives dans la ligne primitive de l'embryon de Poulet. J. Embryol. exp. Morph.23, 79–108 (1970)Google Scholar
  14. Rao, B. R.: The appearance and extension of neural differentiation tendencies in the neurectoderm of the early chick embryo. Wilhelm Roux' Archiv160, 187–236 (1968)Google Scholar
  15. Rawles, M. E.: A study in the localization of organ-forming areas in the chick blastoderm of the head-process stage. J. exp. Zool.72, 271–315 (1936)Google Scholar
  16. Rosenquist, G. C.: A radioautographic study of labeled grafts in the chick blastoderm. Development from primitive-streak stages to stage 12. Contr. Embryol.38, 71–110 (1966)Google Scholar
  17. Rosenquist, G. C.: The origin and movement of nephrogenic cells in the chick embryo as determined by radioautographic mapping. J. Embryol. exp. Morph.24, 367–380 (1970)PubMedGoogle Scholar
  18. Rosenquist, G. C.: The location of the pregut endoderm in the chick embryo at the primitive streak stage as determined by radioautographic mapping. Develop. Biol.26, 323–335 (1971)PubMedGoogle Scholar
  19. Rosenquist, G. C.: Endoderm movements in the chick embryo between the early short streak and head process stages. J. exp. Zool.180, 95–104 (1972)PubMedGoogle Scholar
  20. Spratt, N. T., Jr.: Formation of the primitive streak in the explanted chick blastoderm marked with carbon particles. J. exp. Zool.103, 259–304 (1946)Google Scholar
  21. Spratt, N. T., Jr.: Localization of the prospective neural plate in the early chick blastoderm. J. exp. Zool.120, 109–130 (1952)Google Scholar
  22. Spratt, N. T., Jr.: Analysis of the organizer center in the early chick embryo. I. Localization of prospective notochord and somite cells. J. exp. Zool.128, 121–163 (1955)Google Scholar
  23. Spratt, N. T. Jr.: Analysis of the organizer center in the early chick embryo. II. Studies of the mechanics of notochord elongation and somite formation. J. exp. Zool.134, 577–612 (1957)PubMedGoogle Scholar
  24. Vakaet, L.: Cinephotomicrographic investigations of gastrulation in the chick blastoderm. Arch. Biol.81, 387–426 (1970)Google Scholar
  25. Viswanath, J. R., Mulherkar, L.: Studies on self-differentiating and induction capacities of Hensen's node using intracoelomic grafting technique. J. Embryol. exp. Morph.28, 547–558 (1972)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Marianne Veini
    • 1
    • 2
  • K. Hara
    • 1
  1. 1.Hubrecht LaboratoryUtrechtNetherlands
  2. 2.Zoological LaboratoryUniversity of Athens Panepistimiopolis (Kouponia)AthensGreece

Personalised recommendations