Wilhelm Roux's archives of developmental biology

, Volume 187, Issue 4, pp 355–366 | Cite as

Development ofDentalium following removal of D-quadrant blastomeres at successive cleavage stages

  • James Newton Cather
  • Nicolaas Hendrikus Verdonk
Article

Summary

Each primary micromere and macromere of the D-quadrant ofDentalium was deleted, through the mesentoblast stage, to investigate the way in which the polar lobe cytoplasm exerts its influence on development.-D and -1D embryos form an apical tuft but no posttrochal structures.-2D embryos form an apical tuft and a reduced posttrochal region without a shell. -3D and -4D are externally similar to control embryos. -1d embryos and -1c embryos have an apical tuft with a reduced number of cilia. Embryos in which both 1c and 1d are deleted lack the apical tuft.-2d embryos lack shell and most other posstrochal structures. -3d and-4d embryos appear externally equivalent to controls.

The polar lobe cytoplasm exerts its influence sequentially, and as inIlyanassa the maximal effect is at the third quartet stage.

Key words

Mollusca Embryology experimental Development analysis Dentalium Invertebrate development 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Biggelaar, J.A.M. van den: Development of dorsoventral polarity and mesentoblast determination inPatella vulgata. J. Morphol.154, 157–186 (1977)Google Scholar
  2. Cather, J.N.: Cellular interactions in the development of the shell gland of the gastropod,Ilyanassa. J. Exp. Zool.166, 205–224 (1967)Google Scholar
  3. Cather, J.N.: Cellular interactions in the regulation of development in annelids and molluscs. Adv. Morphog.9, 67–125 (1971)Google Scholar
  4. Cather, J.N., Verdonk, N.H.: The development ofBithynia tentaculata (Prosobranchia, Gastropoda) after removal of the polar lobe. J. Embryol. Exp. Morphol.31, 415–422 (1974)Google Scholar
  5. Cather, J.N., Verdonk, N.H., Dohmen, M.R.: Role of the vegetal body in the regulation of development inBithynia tentaculata (Prosobranchia, Gastropoda). Am. Zool.16, 455–468 (1976)Google Scholar
  6. Clement, A.C.: Development ofIlyanassa following removal of the D macromere at successive cleavage stages. J. Exp. Zool.149, 193–216 (1962)Google Scholar
  7. Clement, A.C.:Ilyanassa. In: Experimental Embryology of Marine and Fresh-water Invertebrates (G. Reverberi, ed.) Amsterdam: North-Holland Publ. 1971Google Scholar
  8. Dohmen, M.R., Verdonk, N.H.: The structure of a morphogenetic cytoplasm, present in the polar lobe ofBithynia tentaculata (Gastropoda, Prosobranchia). J. Embryol. Exp. Morphol.31, 423–433 (1974)Google Scholar
  9. Dohmen, M.R., Verdonk, N.H.: The ultrastructure and role of the polar lobe in development of molluscs. In: Determinants of spatial organization (S. Subtelny and I.R. Konigsberg, eds.). New York: Academic Press (1979)Google Scholar
  10. Dongen, C.A.M. van: The development ofDentalium with special reference to the significance of the polar lobe. V and VI. Differentiation of the cell pattern in lobeless embryos ofDentalium vulgare (da Costa) during late larval development. Proc. K. Ned. Akad. Wet.C79, 245–266 (1976a)Google Scholar
  11. Dongen, C.A.M. van: The development ofDentalium with special reference to the significance of the polar lobe. VII. Organogenesis and histogenesis in lobeless embryos ofDentalium vulgare (da Costa) as compared to normal development. Proc. K. Ned. Akad. Wet.C79, 454–465 (1976b)Google Scholar
  12. Dongen, C.A.M. van: Mesoderm formation during normal development ofDentalium dentale Proc. K. Ned. Akad. Wet.C80, 372–376 (1977)Google Scholar
  13. Dongen, C.A.M. van, Geilenkirchen, W.L.M.: The development ofDentalium with special reference to the significance of the polar lobe. I, II, and III. Division chronology and development of the cell pattern inDentalium dentale (Scaphopoda). Proc. K. Ned. Akad. Wet. AmsterdamC77, 57–100 (1974)Google Scholar
  14. Dongen, C.A.M. van, Geilenkirchen, W.L.M.: The development ofDentalium with special reference to the significance of the polar lobe. IV. Division chronology and development of the cell pattern inDentalium dentale after removal of the polar lobe at first cleavage. Proc. K. Ned. Akad. Wet., AmsterdamC78, 358–375 (1975)Google Scholar
  15. Geilenkirchen, W.L.M., Verdonk, N.H., Timmermans, L.P.M.: Experimental studies on morphogenetic factors localized in the first and second polar lobe onDentalium eggs. J. Embryol. Exp. Morphol.23, 237–243 (1970)Google Scholar
  16. Hess, O.: Entwicklungsphysiologie der Mollusken. Fortschr. Zool.14, 130–163 (1962)Google Scholar
  17. Hess, O.: Fresh water gastropoda. In: Experimental Embryology of Marine and Fresh-water Invertebrates (G. Reverberi, ed.) Amsterdam: North-Holland Publ. 1971Google Scholar
  18. Kowalewsky, A.: Etude sur l'embryogenie du Dentale. Ann. Mus. d'Hist. Nat. Marseille. Zool.1, 7 (1883)Google Scholar
  19. Morrill, J.B., Blair, C.A., Larsen, W.J.: Regulative development in the pulmonate gastropodLymnaea palustris as determined by blastomere deletion experiments. J. Exp. Zool183, 47–56 (1973)Google Scholar
  20. Raven, C.P.: Morphogenesis inLimnaea stagnalis and its disturbance by lithium. J. Exp. Zool.121, 1–77 (1952)Google Scholar
  21. Reverberi, G.: The ultrastructure ofDentalium egg at the trefoil stage. Acta Embryol. Exp.12, 31–43 (1970)Google Scholar
  22. Reverberi, G.:Dentalium. In: Experimental Embryology of Marine and Fresh-water Invertebrates (G. Reverberi, ed.), Amsterdam: North-Holland Publ. 1971Google Scholar
  23. Reverberi, G.: The fine structure of the ovaric egg ofDentalium. Acta Embryol. Exp.14, 135–166 (1972)Google Scholar
  24. Timmermans, L.P.M., Geilenkirchen, W.L.M., Verdonk, N.H.: Local accumulation of Feulgenpositive granules in the egg cortex ofDentalium dentale L. J. Embryol. Exp. Morphol.23, 245–252 (1970)Google Scholar
  25. Verdonk, N.H.: The effect of removing the polar lobe in centrifuged eggs ofDentalium. J. Embryol. Exp. Morphol.19, 33–42 (1968a)Google Scholar
  26. Verdonk, N.H.: The relation of the two blastomeres to the polar lobe inDentalium. J. Embryol. Exp. Morphol.20, 101–105 (1968b)Google Scholar
  27. Verdonk, N.H., Geilenkirchen, W.L.M., Timmermans, L.P.M.: The localization of morphogenetic factors in uncleaved eggs ofDentalium. J. Embryol. Exp. Morphol.25, 57–63 (1971)Google Scholar
  28. Verdonk, N.H., Cather, J.N.: The development of isolated blastomeres inBithynia tentaculata (Prosobranchia, Gastropoda). J. Exp. Zool.186, 47–62 (1973)Google Scholar
  29. Wilson, E.B.: Experimental studies on germinal localization. I. The germ regions in the egg ofDentalium. J. Exp. Zool.1, 1–72 (1904)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • James Newton Cather
    • 1
  • Nicolaas Hendrikus Verdonk
    • 2
  1. 1.Division of Biological ScienceThe University of MichiganAnn ArborUSA
  2. 2.Zoological LaboratoryThe University of UtrechtUtrechtThe Netherlands

Personalised recommendations