Summary
The total soluble protein content in crude extracts of gonads ofHelix aspersa Müller from different stages of its life cycle was quantified and then resolved into different protein fractions using polyacrylamide gel electrophoresis. It was found that both the protein content and wet weight of the gonads increased during growth but decreased markedly during the copulation and egg-laying phases. Electrophoretic analysis of the extracts from different stages revealed that additional fractions progressively appeared and that in adults, the number and comcentration of the bands changed in response to physiological conditions (activity, copulation, egg-laying or hibernation) so that the maximum number of 27 fractions was observed during the active phase. We have attempted to correlate these biochemical observations with previously published descriptions of histological changes that occur during the development of the gonad.
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References
Bayne CJ (1966) Observations on the composition of the layers of the egg ofAgriolimax reticulatus, the grey field slug (Pulmonata, Stylommatophora). Comp Biochem Physiol 19:317–338
Cawood AH, Potter U, Dickinson HG (1978) An evaluation of coomassie brillant blue as a stain for quantitative microdensitometers of protein insection. J Histochem Cytochem 26:645–650
Clarke JT (1964) Simplified “Disc” (polyacrylamide gel) electrophoresis. Ann New York Acad Sci 121:428–436
Davis BJ (1964a) Disc electrophoresis. I. Background and theory. Ann. New York Acad Sci 121:321–349
Davis BJ (1964b) Disc electrophoresis. II Method and application to human serum proteins. Ann New York Acad Sci 121:404–427
Enee J (1980) Contribution à l'étude de la croissance de l'EscargotHelix aspersa Müll.: ontogenèse de l'appareil génital et recherche cytologique sur la différenciation et la sécrétion de la prostate. Thèse 3e cycle, Besançon, no 341
Gatenby JB (1917) The cytoplasmic inclusions of the germ-cells. IIHelix aspersa. Quart J Micr Sc 62:555–611
Gomot L (1977) L'hermaphrodisme chez les mollusques gastéropodes. In: Klotz HP (ed) Problèmes actuels d'endocrinologie et de nutrition. L' Expansion Scientifique Fraçaise, Paris, pp 323–353
Gomot L, Enee J (1978) Biologie de la reproduction de l'EscargotHelix aspersa Müll.: les phases de croissance et la différenciation sexuelle. Atti IV Congresso SMI. Siena 73–85
Guyard A (1971) Etude de la différenciation de l'ovotestis et des facteurs contrôlant l'orientation sexuelle des gonocytes de l'EscargotHelix aspersa Müller. Thèse Doct. Sci Nat, Besançon, no 56
Lowry OH, Rosebrough NJ, Farr AL, and Randall AJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265
Ornstein L (1962) Theory of disc electrophoresis. Reprint from Canalco, Bethesda, Maryland, 1–44
Ramasubramanian K (1979) Qualitative and quantitative studies on proteins of reproductive glands and egg envelopes of the snailAchatina fulica. Indian J Exp Biol 17:1219–1223
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Bôle-Richard, MA., Griffond, B. A quantitative and qualitative study of proteins of the gonadal extract during growth and phases of reproductive activity in the snailHelix aspersa Müller. Wilhelm Roux' Archiv 191, 91–94 (1982). https://doi.org/10.1007/BF00848445
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DOI: https://doi.org/10.1007/BF00848445