Summary
Electron microscopic analysis of primary cultures derived from larvalXenopus liver has shown that these cells, although they form only two-dimensional aggregates, retain and presumably also develop structural characteristics typical of liver parenchyma cells, such as bile canaliculi with microvilli and epithelial junctional complexes. As judged from structural criteria, primary cultures contain 80–90% hepatocytes. In contrast to the intact tissue, primary cultures showed excessive development of microfilaments, however.
Incorporation of labeled amino acids has revealed further that the capacity for protein synthesis is maintained in culture and that synthesis of liverspecific protein albumin is maintained in vitro, even in liver cultures derived from thyrostatic tadpoles. This latter result suggests that initiation of albumin synthesis in the larval liver is probably not dependent upon thyroid hormones but rather reflects the protodifferentiated state of this tissue.
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Berridge, M.V., Farmer, S.R., Green, C.D., Henshaw, E.C., Tata, J.R.: Characterization of polysomes from Xenopus liver synthesizing vitellogenin and translation of vitellogenin and albumin messenger RNA's in vitro. Eur. J. Biochem.62, 161–172 (1976)
Berridge, M.V., Lane, C.D.: Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins. Cell8, 283–297 (1976)
Bisbee, C.A., Baker, M.A., Wilson, A.C., Hadzi-Azimi J., Fischberg, M.: Albumin phylogeny for clawed frogs (Xenopus). Science195, 785–787 (1977)
Bragina, E.E., Vasliev, Ju.M., Gelfand, I.M.: Formation of bundles of microfilaments during spreading of fibroblasts on the substrate. Exp. Cell Res.97, 241–248 (1976)
Chen, P.S.: Patterns and metamorphic changes of serum proteins in amphibia. Wilhelm Roux' Archiv165, 132–149 (1970)
Davis, B.J.: Disc electrophoresis. II. Method and application to human serum protein. Ann. N.Y. Acad. Sci.121, 404–427 (1964)
Di Pasquale, A.: Locomotion of epithelial cells. Exp. Cell Res.95, 425–439 (1975)
Fairbanks, G., Stesick, T.L., Wallach, D.F.H.: Electrophoresis analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry10, 2606–2617 (1971)
Frieden, E., Just, J.J.: Hormone responses in amphibian metamorphosis. In: Mechanisms of Hormone Action (G. Litwack, ed.), Vol. 1, pp. 1–52. New York: Academic Press 1970
Goldman, R.D., Lazarides, E., Pollack, R., Weber, K.: The distribution of actin in non-muscle cells. Exp. Cell. Res.90, 333–344 (1975)
Grieninger, G., Granick, S.: Synthesis and differentiation of plasma proteins in cultured embryonic chicken liver cells: A system for study of regulation of protein synthesis. Proc. Natl. Acad. Sci. USA72, 5007–5011 (1975)
Jeejeebhoy, K.N., Ho, J., Greenberg, G.R., Phillips, M.J., Bruce-Robertson, A., Sodtke, U.: Albumin fibrinogen and transferrin synthesis in isolated rat hepatocyte suspensions. Biochem. J.146, 141–155 (1975)
Kistler, A., Weber, R.: A combined biochemical and morphometric study on tissue changes in Xenopus larvae during induced metamorphosis. Mol. cell. Endocrinol.2, 261–288 (1975)
Laemmli, U.K., Favre, M.: Maturation of the head of bacteriophage T4. J. Mol. Biol.80, 575–599 (1973)
Laskey, R.A., Mills, A.D.: Quantitative film detection of3H and14C in polyacrylamide gels by fluorography. Eur. J. Biochem.56, 335–341 (1975)
Lemonnier, F., Cornec, M.L., Golovtchenko, M., Gautier, M.: Synthèse de l'albumine au cours de cultures issues de parenchyme hépatique d'origine humaine. Biochimie56, 1111–1117 (1974)
Ouchterlony, O., Nilsson, L.A.: In: Handbook of Experimental Immunology (D.M. Weir ed.). Oxford Blackwell Scientific 1973
Périssel, B., Chassebeuf, M., Malet, P., Padieu, P.: Etude ultrastructurale d'hépatocytes de rat nouveau-né en culture monocouche établie. C.R. Acad. Sci.277, 2429–2432 (1973)
Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–212 (1967)
Rutter, W.J., Wessels, N.K., Grobstein, C.: Control of specific synthesis in the developing pancreas. Nat. Canc. Inst. Monogr. No.13, pp. 51–65 (1964)
Travis, J., Bowen, J., Tewksbury, D., Johnson, D., Pannell, R.: Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma. Biochem. J.157, 301–306 (1976)
Wahli, W., Weber, R.: Factors promoting the establishment of primary cultures of liver cells from Xenopus larvae. Wilhelm Roux's Archives182, 347–360 (1977)
Wanson, J.-C., Drochmans, P., Mosselmans, R., Ronceveaux, M.F.: Adult rat hepatocytes in primary monolayer culture. Ultrastructural characteristics of intercellular contacts and cell membrane differentiation. J. Cell Biol.74, 858–877 (1977)
Weber, R.: Biochemistry of amphibian metamorphosis. In: The Biochemistry of Animal Development (R. Weber, ed.), Vol. 2, pp. 277–301, New York, Academic Press 1967
Weeke, B.: General Remarks on Principles Equipment, Reagents and Procedures. In: Quantitative Immunoelectrophoresis (N.H. Axelsen, J. Krøll, and B. Weeke, eds.), pp. 15–36 Oslo: Universitetsforlaget 1973
Weigand, K., Müller, M., Urban, J., Schreiber, G.: Intact endoplasmic reticulum and albumin synthesis in rat liver cell suspensions. Exp. Cell Res.67, 27–32 (1971)
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Wahli, W., Abraham, I. & Weber, R. Retention of the differentiated state by larvalXenopus liver cells in primary culture. Wilhelm Roux' Archiv 185, 235–248 (1978). https://doi.org/10.1007/BF00848354
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DOI: https://doi.org/10.1007/BF00848354