Binucleate cells in mouse morulae

  • Maria Salomea Soltyńska
  • Hanna Balakier
  • Anna Witkowska
  • Jolanta Karasiewicz
Rapid Communications


Mouse morulae from two strains were examined in whole mounts after dissociation of embryos into single cells and were analysed in serial sections by light and electron microscopy. One or two binucleate cells per embryo were discovered in a statistically significant number of morulae. The frequency of morulae with binucleate cell(s) was higher in older morulae than in younger ones. Binucleate cells were always the outer cells of the embryo. Their ultrastructure did not differ from the ultrastructure of mononucleate cells. It is suggested that cell binuclearity at the morula stage is a possible way to polyploidization of nuclei, resulting in the formation of primary trophoblast giant cells.

Key words

Mouse Morula Binucleate cell Polyploidization Trophoblast 


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  1. Barlow P, Owen DAJ, Graham C (1972) DNA synthesis in the preimplantation mouse embryo. J Embryol Exp Morphol 27:431–445PubMedGoogle Scholar
  2. Böhm N, Noltemeyer N (1981) Development of binuclearity and DNA-polyploidization in the growing mouse liver. Histochemistry 72:55–61PubMedGoogle Scholar
  3. Calarco PG, Brown EH (1969) An ultrastructural and cytological study of preimplantation development of the mouse. J Exp Zool 171:253–283PubMedGoogle Scholar
  4. Ducibella T, Ukena T, Karnovsky M, Anderson E (1977) Changes in cell surface and cortical cytoplasmic organization during early morphogenesis in the preimplantation mouse embryo. J Cell Biol 74:153–167PubMedGoogle Scholar
  5. Dziadek MA (1981) Use of glycine as a non-enzymic procedure for separation of mouse embryonic tissues and dissociation of cells. Exptl Cell Res 133:383–393PubMedGoogle Scholar
  6. Fakan S, Odartchenko N (1980) Ultrastructural organization of the cell nucleus in early mouse embryos. Biol Cell 37:211–218Google Scholar
  7. Hillman N, Hillman R (1975) Ultrastructural studies of tw32/tw32 mouse embryos. J Embryol Exp Morphol 33:685–695PubMedGoogle Scholar
  8. Hillman N, Tasca RJ (1969) Ultrastructural and autoradiographic studies of mouse cleavage. Am J Anat 126:151–174PubMedGoogle Scholar
  9. Hillman N, Hillman R Wileman G (1970) Ultrastructural studies of cleavage stage t12/t12 mouse embryo. Am J Anat 128:311–340PubMedGoogle Scholar
  10. Ilgren EB (1980) Polyploidization of extraembryonic tissues during mouse embryogenesis. J Embryol Exp Morphol 59:103–111PubMedGoogle Scholar
  11. Ilgren EB (1981a) The initiation and control of trophoblastic growth in the mouse: binucleation and polyploidy. Placenta 2:317–332PubMedGoogle Scholar
  12. Ilgren EB (1981b) On the control of the trophoblastic giant-cell transformation in the mouse: homotypic cellular interaction and polyploidy. J Embryol Exp Morphol 62:183–202PubMedGoogle Scholar
  13. Johnson MH, Ziomek CA (1981) The foundation of the two distinct cell lineages within the mouse morula. Cell 24:71–80PubMedGoogle Scholar
  14. MacQueen HA, Johnson MH (1983) The fifth cell cycle of the mouse embryo is longer for smaller cells than for larger cells. J Embryol Exp Morphol 77:297–308PubMedGoogle Scholar
  15. Saccoman FM, Morgan ChF, Wells LJ (1967) Radioautographic studies of DNA synthesis in the developing extraembryonic membranes of the mouse. Anat Rec 158:197–206PubMedGoogle Scholar
  16. Sherman MJ, Atienza-Samols SB (1979) Differentiation of mouse trophoblast does not require cell-cell interaction. Exp Cell Res 123:73–77PubMedGoogle Scholar
  17. Sherman MJ, McLaren A, Walker PMB (1972) Mechanism of accumulation of DNA in giant cells of mouse trophoblast. Nature [Lond] 238:175–176Google Scholar
  18. Sołtyńska MS (1982) The possible mechanism of cell positioning in mouse morulae: an ultrastructural study. J Embryol Exp Morphol 68:137–147PubMedGoogle Scholar
  19. Stern S, Biggers JD, Anderson E (1971) Mitochondria and early development of the mouse. J Exp Zool 176:179–192PubMedGoogle Scholar
  20. Tarkowski AK (1966) An air-drying method for chromosome preparations from mouse eggs. Cytogenetics 5:394–400Google Scholar
  21. Zybina EV (1970) Features of polyploidization patterns of trophoblast cells. Tsitologiya 12:1081–1096Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Maria Salomea Soltyńska
    • 1
  • Hanna Balakier
    • 2
  • Anna Witkowska
    • 3
  • Jolanta Karasiewicz
    • 4
  1. 1.Department of Cytology, Zoological InstituteUniversity of WarsawWarszawaPoland
  2. 2.Department of ZoologyUniversity of TorontoTorontoCanada
  3. 3.Department of Histology and EmbryologyFaculty of Veterinary MedicineWarszawa
  4. 4.Department of Embryology, Zoological InstituteUniversity of WarsawWarszawaPoland

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