Wilhelm Roux's archives of developmental biology

, Volume 184, Issue 3, pp 233–249 | Cite as

Differentiation in vitro of larval and adult muscles from embryonic cells ofDrosophila

  • Andreas Dübendorfer
  • Annemarie Blumer
  • Ilan Ivan Deàk
Article

Summary

The differentiation of muscles in primary cultures of cells fromDrosophila melanogaster embryos was investigated. In early cultures, and in the absence of exogenous ecdysone, two main classes of muscle were found. Comparison, by light and electron microscopy, of one of these classes (the “myotube” class) with muscles from third instar larvae shows that this class corresponds to the muscles of the body wall of the larva. When α- or β-ecdysone is added to the cultures, these undergo a number of metamorphic changes. Most of the larval muscles disappear, and two new types of muscle form. Ultrastructural and light microscopic examination of these two types indicates that they correspond to the two classes of skeletal muscle (fibrillar and tubular) found in adult flies.

Key words

Tissue culture Muscles Metamorphosis Ecdysone Drosophila 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Auber, J.: La myofibrillogenèse du muscle strié—I. Insectes. J. Microsc.8, 197–223 (1969)Google Scholar
  2. Beinbrech, G.: Elektronenmikroskopische Untersuchungen über die Differenzierung von Insektenmuskeln während der Metamorphose. Z. Zellforsch.90, 463–494 (1968)PubMedGoogle Scholar
  3. Crossley, A.C.: The fine structure and mechanism of breakdown of larval intersegmental muscles in the blowflyCalliphora erythrocephala. J. Insect Physiol.14, 1389–1407 (1968)Google Scholar
  4. Deak, I.I.: Mutations ofDrosophila melanogaster that affect muscles. J. Embryol. Exp. Morphol.40, 35–63 (1977)PubMedGoogle Scholar
  5. Deak, I.I.: Thoracic duplications in the mutantwingless ofDrosophila and their effect on muscles and nerves. Dev. Biol., 1978 (accepted per publication)Google Scholar
  6. Donady, J.J., Seecof, R.L.: Effect of the genelethal (1)myospheroid onDrosophila embryonic cells in vitro. In Vitro8, 7–12 (1972)PubMedGoogle Scholar
  7. Dübendorfer, A.: Metamorphosis of imaginal disc tissue grown in vitro from dissociated embryos ofDrosophila. In: Invertebrate Tissue Culture. Applications in Medicine, Biology and Agriculture. New York: Acad. Press 1976Google Scholar
  8. Dübendorfer, A., Shields, G., Sang, J.H.: Development and differentiation in vitro ofDrosophila imaginal disc cells from dissociated early embryos. J. Embryol. Exp. Morphol.33, 487–498 (1975)PubMedGoogle Scholar
  9. Elder, H.Y.: In: Insect muscle (P.N.R. Usherwood, ed.), New York: Acad. Press 1975Google Scholar
  10. Gerson, I., Seecof, R.L., Teplitz, R.L.: Ultrastructural differentiation during embryonicDrosophila myogenesis in vitro. In Vitro12, 615–622 (1976)PubMedGoogle Scholar
  11. Goldstein, M.A.: An ultrastructural study of supercontraction in the body wall muscles ofDrosophila melanogaster larvae. Anat. Rec.169, 326 (1971)Google Scholar
  12. Goldstein, M.A., Burdette, W.J.: Striated visceral muscle ofDrosophila melanogaster. J. Morphol.134, 315–334 (1971)PubMedGoogle Scholar
  13. Graham-Smith, G.S.: The alimentary canal ofCalliphora erythrocephala L., with special reference to its musculature and to the proventriculus, rectal value and rectal papillae. Parasitology26, 176–248 (1934)Google Scholar
  14. Hotta, Y., Benzer, S.: Mapping of behaviour inDrosophila mosaics. Nature (London)240, 527–535 (1972)Google Scholar
  15. Miller, A.: In: Biology ofDrosophila (M. Demerec, ed.) New York: Hafner 1950Google Scholar
  16. Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. Appl. Phys.32, 1637 (1961)Google Scholar
  17. Nüesch, H.: The role of the nervous system in insect morphogenesis and regeneration. A. Rev. Ent.13, 27–44 (1968)Google Scholar
  18. Osborne, M.P.: Supercontraction in the muscles of the blowfly larva: an ultrastructural study. J. Insect Physiol.13, 1471–1482 (1967)Google Scholar
  19. Pasquali-Ronchetti, I.: The ultrastructural organization of femoral muscles inMusca domestica (Diptera). Cell Tiss. Res.2, 339–354 (1970)Google Scholar
  20. Robertson, C.W.: The metamorphosis ofDrosophila melanogaster, including an accurately timed account of the principal morphological changes. J. Morphol.59, 351–400 (1936)Google Scholar
  21. Sahota, T.S., Beckel, W.E.: The influence of epidermis on the developing flight muscles inGalleria mellonella. Can. J. Zool.45, 421–434 (1967)Google Scholar
  22. Seecof, R.L., Alléaume, N., Teplitz, R.L., Gerson, I.: Differentiation of neurons and myocytes in cell cultures made fromDrosophila gastrulae. Exp. Cell Res.69, 161–173 (1971)PubMedGoogle Scholar
  23. Seecof, R.L., Gerson, I., Donady, J.J., Teplitz, R.L.:Drosophila myogenesis in vitro: the genesis of “small” myocytes and myotubes. Dev. Biol.35, 250–261 (1973)PubMedGoogle Scholar
  24. Seecof, R.L., Teplitz, R.L., Gerson, I., Ikeda, K., Donady, J.J.: Differentiation of neuromuscular junctions in cultures of embryonicDrosophila cells. Proc. Natl. Acad. Sci. USA62, 566–570 (1972)Google Scholar
  25. Seecof, R.L., Unanue, R.L.: Differentiation of embryonicDrosophila cells in vitro. Exp. Cell Res.50, 654–660 (1968)PubMedGoogle Scholar
  26. Shafiq, S.A.: Electron microscopic studies on the indirect flight muscles ofDrosophila melanogaster. I. Structure of the myofibrils. J. Cell Biol.17, 351–362 (1963a)PubMedGoogle Scholar
  27. Shafiq, S.A.: Electron microscopic studies on the indirect flight muscles ofDrosophila melanogaster. II. Differentiation of myofibrils. J. Cell Biol.17, 363–373 (1963b)PubMedGoogle Scholar
  28. Shields, G., Dübendorfer, A., Sang, J.H.: Differentiation in vitro of larval cell types from early embryonic cells ofDrosophila melanogaster. J. Embryol. Exp. Morphol.33, 159–175 (1975)PubMedGoogle Scholar
  29. Shields, G., Sang, J.H.: Characteristics of five cell types appearing during in vitro culture of embryonic material fromDrosophila melanogaster. J. Embryol. Exp. Morphol.23, 53–69 (1970)PubMedGoogle Scholar
  30. Shields, G., Sang, J.H.: Improved medium for culture ofDrosophila embryonic cells. Dros. Inform. Serv.52, 161 (1977)Google Scholar
  31. Tiegs, O.W.: The flight muscles of insects — their anatomy and histology; with some observations on the structure of striated muscle in general. Phil. Trans. Roy. Soc., London, Ser. B.238, 221–348 (1955)Google Scholar
  32. Usherwood, P.N.R.: Insect muscle. New York: Acad. Press 1975Google Scholar
  33. Yaffe, D.: Cellular aspects of muscle differentiation in vitro. Curr. Top. Dev. Biol.4, 37–77 (1969)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Andreas Dübendorfer
    • 1
  • Annemarie Blumer
    • 1
  • Ilan Ivan Deàk
    • 1
  1. 1.Zoologisches Institut der Universität ZürichZürichSwitzerland

Personalised recommendations