Wilhelm Roux's archives of developmental biology

, Volume 184, Issue 3, pp 233–249 | Cite as

Differentiation in vitro of larval and adult muscles from embryonic cells ofDrosophila

  • Andreas Dübendorfer
  • Annemarie Blumer
  • Ilan Ivan Deàk


The differentiation of muscles in primary cultures of cells fromDrosophila melanogaster embryos was investigated. In early cultures, and in the absence of exogenous ecdysone, two main classes of muscle were found. Comparison, by light and electron microscopy, of one of these classes (the “myotube” class) with muscles from third instar larvae shows that this class corresponds to the muscles of the body wall of the larva. When α- or β-ecdysone is added to the cultures, these undergo a number of metamorphic changes. Most of the larval muscles disappear, and two new types of muscle form. Ultrastructural and light microscopic examination of these two types indicates that they correspond to the two classes of skeletal muscle (fibrillar and tubular) found in adult flies.

Key words

Tissue culture Muscles Metamorphosis Ecdysone Drosophila 


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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Andreas Dübendorfer
    • 1
  • Annemarie Blumer
    • 1
  • Ilan Ivan Deàk
    • 1
  1. 1.Zoologisches Institut der Universität ZürichZürichSwitzerland

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